{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T13:35:35Z","timestamp":1761831335390,"version":"build-2065373602"},"reference-count":34,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND  Chagas disease (CD) caused by Trypanosoma cruzi has limited therapy. Probiotics sustain healthy microbiota, playing roles in biological events.  OBJECTIVES  Our aim was to determine the impact of probiotics on T. cruzi infection in vitro and in mouse acute experimental models.  METHODS  The multi strain - PB8 and the Lactobacillus rhamnosus (LR) were orally administered [106-109 colony-forming units (CFU)] for seven days prior to mice infection followed for 14 daily administrations. Peritoneal mouse macrophages (PMM) were obtained from mice treated with 109 probiotics one day before collection and infected in vitro with or not benznidazole (BZ).  FINDINGS  LR and PB8 reduced by 44-87% and 23-16% the parasitaemia peak in male and female mice, respectively, but did not protect against mortality. Histopathology showed mild reduction in cardiac nests due to probiotics\u2019 administration. PB8 and LR suppressed the parasite infection of PMM by 24 and 26%, reaching 65 and 42% of declines, respectively when 3% thioglycolate was performed. PB8 increased BZ activity at 1 \u00b5M, reaching 40% of parasitism\u2019 declines compared to BZ alone (25%). No gender difference was noticed during probiotic in vivo administration.  MAIN CONCLUSIONS  The results point to the potential of a combined therapeutic approach for CD, using probiotics and BZ.<\/jats:p>","DOI":"10.1590\/0074-02760240272","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T13:32:19Z","timestamp":1761831139000},"source":"Crossref","is-referenced-by-count":0,"title":["The impact of probiotic administration on experimental in vitro and in vivo infection by Trypanosoma cruzi"],"prefix":"10.1590","volume":"120","author":[{"given":"Denise da Gama Ja\u00e9n","family":"Batista","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara Calheiros","family":"Missagia","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly Cristina","family":"Demarque","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Melo","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Meuser","family":"Batista","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-6106","authenticated-orcid":false,"given":"Maria de Nazar\u00e9 Correia","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","year":"2024"},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.5772\/intechopen.84472","article-title":"Parasite, compartments, and molecules: trick versus treatment on Chagas disease","author":"Vannier-Santos MA","year":"2019","journal-title":"Biology of Trypanosoma cruzi"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1590\/S0074-02761909000200008","article-title":"Nova tripanozomiase humana Estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homem","volume":"1","author":"Chagas C","year":"1909","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref4","doi-asserted-by":"crossref","DOI":"10.5772\/intechopen.84639","article-title":"Life cycle of Trypanosoma cruzi in the invertebrate and the vertebrate 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