{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T23:51:13Z","timestamp":1775001073710,"version":"3.50.1"},"reference-count":42,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UBA","award":["20020120200140"],"award-info":[{"award-number":["20020120200140"]}]},{"name":"UBA","award":["20020130200137BA"],"award-info":[{"award-number":["20020130200137BA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p> BACKGROUND Trypanosoma cruzi, causative agent of Chagas disease (CD), remains a public health problem in Latin America and is emerging in non-endemic areas. Phospholipids (PL) are essential components of biomembranes and their enzymatic modification by phospholipases yields bioactive lipids that modulate immune responses. Anti-PL antibodies have been associated with autoimmune diseases and inflammation, potentially influencing CD pathology by recognising PL and PL-binding proteins. T. cruzi Phospholipase A1 (TcPLA1) hydrolyses membrane PL and participates in parasite-host cell interactions.  OBJECTIVES  This study evaluated IgM and IgG antibody responses against phosphatidylcholine, phosphatidylethanolamine, and their derived lysophospholipids (LPL), as well as recombinant TcPLA1, during experimental T. cruzi infection with two strains: RA (high virulence) and K98 (low virulence). It also aimed to predict the recognition capacity of TcPLA1 by CD patients using in silico analysis.  METHODS  Antibody responses were analysed by enzyme-linked immunosorbent assay (ELISA) using different PL and recombinant TcPLA1 as antigens. Lytic activity assays were performed to evaluate the functional impact of anti-PL antibodies. The CHAGASTOPE resource was used to predict TcPLA1 antigenicity.  FINDINGS  This study identified IgM and IgG antibodies against PL, LPL and TcPLA1 during experimental T. cruzi infection. Different amino acid sequences of TcPLA1 showed stronger antigenic recognition by CD patient\u2019s sera.  MAIN CONCLUSIONS  The presence of these antibodies suggests their involvement in the pathogenesis of CD and their potential as markers for disease monitoring and prognosis.<\/jats:p>","DOI":"10.1590\/0074-02760240281","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T17:58:50Z","timestamp":1757354330000},"source":"Crossref","is-referenced-by-count":1,"title":["Phospholipids and phospholipase A1 as antigens during the course of experimental Trypanosoma cruzi infection"],"prefix":"10.1590","volume":"120","author":[{"given":"Emanuel","family":"Bott","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires,  Argentina; Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas-CONICET,  Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n Andr\u00e9s","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires,  Argentina; Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas-CONICET,  Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guadalupe","family":"Gimenez","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires,  Argentina; Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas-CONICET,  Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Elisa","family":"Solana","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires,  Argentina; Universidad Nacional de Luj\u00e1n,  Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Laura","family":"Belaunzar\u00e1n","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires,  Argentina; Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas-CONICET,  Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","year":"2024"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.actatropica.2017.09.017","article-title":"Trypanosoma cruzi genetic diversity something new for something known about Chagas disease manifestations, serodiagnosis and drug sensitivity","volume":"184","author":"Zingales B","year":"2018","journal-title":"Acta Trop"},{"key":"ref3","article-title":"Biochemical mechanisms in the regulation of phospholipases. 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