{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:42:47Z","timestamp":1790685767512,"version":"4.1.0"},"reference-count":19,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["88887.805768\/2023-00"],"award-info":[{"award-number":["88887.805768\/2023-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["88887.637869\/2021-00"],"award-info":[{"award-number":["88887.637869\/2021-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["311309\/2023-2"],"award-info":[{"award-number":["311309\/2023-2"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["306448\/2023-8"],"award-info":[{"award-number":["306448\/2023-8"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["88887.805768\/2023-00"],"award-info":[{"award-number":["88887.805768\/2023-00"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["88887.637869\/2021-00"],"award-info":[{"award-number":["88887.637869\/2021-00"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["311309\/2023-2"],"award-info":[{"award-number":["311309\/2023-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["306448\/2023-8"],"award-info":[{"award-number":["306448\/2023-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND  Chagas disease (CD), a neglected tropical disease caused by Trypanosoma cruzi, remains a significant often underdiagnosed public health challenge in endemic regions, affecting millions globally. Accurate and timely diagnosis is critical, but the performance of existing diagnostic methods varies widely in sensitivity and specificity.  OBJECTIVES  This multicentre study assessed the diagnostic performance of 17 serological assays for detecting anti-T. cruzi antibodies.  METHODS  Commercial enzyme immunoassays (EIA), indirect haemagglutination assays (IHA), indirect immunofluorescence assays (IIF), rapid diagnostic tests (RDT), and a chemiluminescent microparticle immunoassay (CMIA) were included in this study.  FINDINGS  Some EIA-based tests achieved 100% sensitivity, while IHAs and IIFs demonstrated reduced specificity. CMIA exhibited 100% sensitivity, highlighting its potential as a robust screening tool. Combining EIAs with IHAs or IIFs improved overall sensitivity, often surpassing 99%, although specificity remained variable. Cross-reactivity with other parasitic diseases posed challenges to specificity, particularly in assays employing crude antigens.  MAIN CONCLUSIONS  These findings emphasise the importance of tailoring diagnostic tool selection to regional epidemiological contexts and advancing antigen refinement to enhance diagnostic accuracy and accessibility, particularly in resource-limited settings.<\/jats:p>","DOI":"10.1590\/0074-02760240282","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T16:38:50Z","timestamp":1749487130000},"source":"Crossref","is-referenced-by-count":10,"title":["A multicentre comparative study of serological methods for diagnosing Chagas disease in Brazil"],"prefix":"10.1590","volume":"120","author":[{"given":"Alejandro Luquetti","family":"Ostermayer","sequence":"first","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Alvarenga Cardoso","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline Ara\u00fajo Domingos","family":"Iturra","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Job Alves","family":"de Souza Filho","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Maia","family":"Leony","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa de Carvalho Medrado","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane da Rocha","family":"Siriano","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suelene Brito do Nascimento","family":"Tavares","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius Silva","family":"Belo","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a Silvestre","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3944-0818","authenticated-orcid":false,"given":"Fred Luciano Neves","family":"Santos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","year":"2024"},{"key":"ref2","article-title":"Estimating demand for anti-Chagas drugs a contribution for access in Latin America","volume":"41","author":"Chaves GC","year":"2017","journal-title":"Rev Panam Salud Publica"},{"key":"ref3","article-title":"Portaria n\u00ba 57, de 30 de outubro de 2018. 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