{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T15:40:17Z","timestamp":1758555617246,"version":"3.44.0"},"reference-count":72,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p> BACKGROUND  The microbiome is fundamental in the host\u2019s immunobiology and dysbiosis leads to pathological conditions, potentially affecting parasitic diseases.  OBJECTIVES  To investigate how oral probiotics affect infection and antiparasitic treatment of Leishmania in macrophages.  METHODS  Swiss mice were orally treated with 109 colony forming units (CFU) multi- or single-strain probiotic formulations (PB8, Bifilac), their peritoneal mouse macrophages (PMMs) were obtained and infected ex vivo with L. amazonensis amastigotes. The effects of prior probiotic administration on ex vivo infection and treatment responses to 1 \u00b5M miltefosine and N 6-methyltubercidin were evaluated. Flow cytometry measured the inflammatory mediator release in the supernatant of the PMMs.  FINDINGS AND MAIN CONCLUSIONS  PB8 or Bifilac administration significantly reduced (p &lt; 0.05) ex vivo infection of PMMs from male mice by 27% and 12%, respectively. No gender-dependent effect of probiotics was observed. No improved antiparasitic activity of 1 \u00b5M miltefosine or N 6-methyltubercidin was observed in probiotic-treated PMMs. Ex vivo Leishmania infection stimulated tumour necrosis factor (TNF), MCP-1, and interleukin-6 (IL-6) production by PMMs (p &lt; 0.05). A trend of increase was recorded with elevated levels of TNF and IL-6 in PB8-treated male groups (around 43 and 52%, respectively) but were not statistically significant. Collectively, probiotic treatment of mice influences Leishmania infection in PMMs. Clinical applications in leishmaniasis warrant further studies.<\/jats:p>","DOI":"10.1590\/0074-02760250014","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T15:03:49Z","timestamp":1758553429000},"source":"Crossref","is-referenced-by-count":0,"title":["The impact of probiotic administration in vivo on peritoneal mouse macrophages infected by Leishmania amazonensis ex vivo"],"prefix":"10.1590","volume":"120","author":[{"given":"Lauren Van den","family":"Broeck","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; University of Antwerp,  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel Silva","family":"de Azevedo","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludmila Ferreira de Almeida","family":"Fiuza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Meuser","family":"Batista","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia Machado","family":"Cascabulho","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewout","family":"Van de Velde","sequence":"additional","affiliation":[{"name":"Laboratory of Medicinal Chemistry,  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"Van Calenbergh","sequence":"additional","affiliation":[{"name":"Laboratory of Medicinal Chemistry,  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Caljon","sequence":"additional","affiliation":[{"name":"University of Antwerp,  Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-6106","authenticated-orcid":false,"given":"Maria de Nazar\u00e9 Correia","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","year":"2023"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s40475-021-00232-7","article-title":"A review of leishmaniasis current knowledge and future directions","volume":"8","author":"Mann S","year":"2021","journal-title":"Curr Trop Med 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