{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T14:22:40Z","timestamp":1781360560480,"version":"3.54.1"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND Mycobacterium brisbanense is a rare nontuberculous mycobacteria and was for the first time detected in the Americas in humans. However, this, like several other species of mycobacteria, may be underreported worldwide. Therefore, their study is increasingly important.  OBJECTIVES  The aim of this article is to report and analyse the first seven human isolates of M. brisbanense in the Americas, derived from six Brazilian patients.  METHODS  We sequenced the genes hsp65, rpoB and 16s rRNA, of seven mycobacterial clinical isolates, constructed a phylogenetic tree, and determined their drug susceptibility profile.  FINDINGS  The regions sequenced were highly similar between the M. brisbanense type strain and the Brazilian strains. Similarly, their susceptibility profiles were similar to that of M. brisbanense type strain, except for two antibiotics tested, cefoxitin and doxycycline.  MAIN CONCLUSIONS  All studied strains were identified at the species level by a concatenated tree as M. brisbanense.<\/jats:p>","DOI":"10.1590\/0074-02760250063","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T15:02:20Z","timestamp":1761318140000},"source":"Crossref","is-referenced-by-count":2,"title":["First isolation of Mycobacterium brisbanense from humans in the Americas"],"prefix":"10.1590","volume":"120","author":[{"given":"Carlos Eduardo Dias","family":"Campos","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel dos Santos de Sena","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Marco Vicente","family":"da Silva","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Dist\u00e1sio","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa de Almeida Barbosa","family":"Eccard","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle Pinheiro Nobre","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4183-5915","authenticated-orcid":false,"given":"Jesus Pais","family":"Ramos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","article-title":"Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media","author":"Kim CJ","year":"2013","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref2","article-title":"Mycobacterium fortuitum um novo bacillo acidoresistance pathogenico para o homen","author":"Cruz JDC","year":"1938","journal-title":"Acta Medica"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.TNMI7-0027-2016","article-title":"Rapidly growing mycobacteria","author":"Brown-Elliott BA","year":"2017","journal-title":"Microbiol Spectr"},{"key":"ref4","article-title":"Clinical disease, drug susceptibility, and biochemical patterns of the unnamed third biovariant complex of Mycobacterium fortuitum","author":"Wallace RJ","year":"1991","journal-title":"J Infect Dis"},{"key":"ref5","article-title":"Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates","author":"Schinsky MF","year":"2004","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref6","article-title":"Mycobacterium brisbanense lung infection facilitated by steroid induced adrenal insufficiency","author":"Gynthersen R","year":"2018","journal-title":"APMIS"},{"key":"ref7","article-title":"Mycobacterium brisbanense species nova isolated from a patient with chronic cavitary lung infection","author":"Poh ME","year":"2014","journal-title":"Chest"},{"key":"ref8","article-title":"Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup","author":"Khurana JL","year":"2009","journal-title":"Biochem J"},{"key":"ref9","article-title":"Whole-genome sequencing and comparative analysis of Mycobacterium brisbanense reveals a possible soil origin and capability in fertiliser synthesis","author":"Wee WY","year":"2016","journal-title":"PLoS One"},{"key":"ref10","article-title":"Gene sequencing and phylogenetic analysis: powerful tools for an improved diagnosis of fish mycobacteriosis caused by Mycobacterium fortuitum group members","author":"Mugetti D","year":"2021","journal-title":"Microorganisms"},{"key":"ref11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0227759","article-title":"Diversity of Mycobacteriaceae from aquatic environment at the S\u00e3o Paulo Zoological Park Foundation in Brazil","author":"Romagnoli CL","year":"2020","journal-title":"PLoS One"},{"key":"ref12","article-title":"Mycobacterium aquaticum sp. nov., a rapidly growing species isolated from haemodialysis water","author":"Shahraki HA","year":"2017","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref13","article-title":"Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine clinical mycobacteriology laboratory","author":"Hall L","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.41.12.5699-5708.2003","article-title":"RpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria","author":"Ad\u00e9kambi T","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.31.2.175-178.1993","article-title":"Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis","author":"Telenti A","year":"1993","journal-title":"J Clin Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","author":"Altschul SF","year":"1990","journal-title":"J Mol Biol"},{"key":"ref17","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.42.7.3000-3011.2004","article-title":"Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources","author":"McNabb A","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","DOI":"10.4056\/sigs.932152","article-title":"Standard operating procedure for optimal identification of mycobacteria using 16S rRNA gene sequences","author":"Tortoli E","year":"2010","journal-title":"Stand Genomic Sci"},{"key":"ref19","doi-asserted-by":"crossref","DOI":"10.1099\/ijs.0.63222-0","article-title":"A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model","author":"Devulder G","year":"2005","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref20","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","author":"Tamura K","year":"2021","journal-title":"Mol Biol Evol"},{"key":"ref21","article-title":"An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis","author":"Hillis DM","year":"1993","journal-title":"Syst Biol"},{"key":"ref22","article-title":"Susceptibility testing of Mycobacteria, Nocardiae, and other aerobic actinomycetes","year":"2011","journal-title":"Wayne"},{"key":"ref23","article-title":"Performance standards for susceptibility testing of Mycobacteria, Nocardia spp., and other aerobic actinomycetes","year":"2018","journal-title":"Wayne"},{"key":"ref24","article-title":"In vitro drug susceptibility of 40 international reference rapidly growing mycobacteria to 20 antimicrobial agents","author":"Pang H","year":"2015","journal-title":"Int J Clin Exp Med"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1016\/j.ccm.2023.08.012","article-title":"Global epidemiology of nontuberculous mycobacterial pulmonary disease: a review","author":"Prevots DR","year":"2023","journal-title":"Clin Chest Med"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0074-02762025000101152&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T15:02:23Z","timestamp":1761318143000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762025000101152&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":25,"alternative-id":["S0074-02762025000101152"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760250063","relation":{},"ISSN":["1678-8060","0074-0276"],"issn-type":[{"value":"1678-8060","type":"electronic"},{"value":"0074-0276","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e250063"}}