{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T15:04:12Z","timestamp":1763478252085,"version":"3.45.0"},"reference-count":40,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"crossref","award":["SEI 260003\/005902\/2024"],"award-info":[{"award-number":["SEI 260003\/005902\/2024"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"crossref","award":["88881.311598\/2018-01"],"award-info":[{"award-number":["88881.311598\/2018-01"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["SEI 260003\/005902\/2024"],"award-info":[{"award-number":["SEI 260003\/005902\/2024"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["88881.311598\/2018-01"],"award-info":[{"award-number":["88881.311598\/2018-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND Streptococcus agalactiae is responsible for sepsis and meningitis, and the major cause of neonatal morbidity and mortality. However, how S. agalactiae disrupts endothelial barriers is poorly understood.  OBJECTIVES  Analyse the influence of endothelial cell (HUVECs) growth under static and shear stress conditions during infection with S. agalactiae, and the role of pilus PI-2b during endothelial barrier disruption and increased endothelial permeability.  METHODS  HUVECs under static and shear conditions were infected by S. agalactiae (GBS90356 and GBS90356\u0394pilus2b) strains in the presence and absence of fibrinogen. VE-cadherin was evaluated by immunofluorescence and RT-PCR assays, and the endothelial permeability by transwell assay.  FINDS  Shear stress induced the alignment of HUVECs and increased the adherence of S. agalactiae strains (GBS90356 and GBS90356\u0394pilus2b), mainly in the presence of fibrinogen, in addition to greater peripheral localisation of VE-cadherin. Rupture points and damage to endothelial integrity was visualised after infection with the GBS90356WT strain, mainly in the presence of fibrinogen. RT-PCR analyses identified increase in VE-cadherin expression in HUVECs under shear stress and a decrease in VE-cadherin after infection, with increased levels of endothelial permeability.  MAIN CONCLUSION Data demonstrate for the first time the dysfunction of the adhesive barrier induced by the S. agalactiae ST-17 strain, mainly in HUVECs under shear stress, where PI-2b expression was essential to optimise the damage to endothelial integrity.<\/jats:p>","DOI":"10.1590\/0074-02760250077","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T14:59:56Z","timestamp":1763477996000},"source":"Crossref","is-referenced-by-count":0,"title":["Infection of endothelial cells by Streptococcus agalactiae reveals potential role of PI-2b pilus on endothelial barrier dysfunction"],"prefix":"10.1590","volume":"120","author":[{"given":"Jessica Silva Santos","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna Alves da Silva","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Nagao","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o T\u00e9cnico-Educacional Souza Marques,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eduarda","family":"Negreiro e Silva","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o T\u00e9cnico-Educacional Souza Marques,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela da Silva","family":"Santos","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6007-0033","authenticated-orcid":false,"given":"Prescilla Emy","family":"Nagao","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","article-title":"GBS Danish and Dutch collaborative group for long term outcomes; GBS Low and Middle Income Countries collaborative group for long term outcomes; GBS Scientific Advisory Group, epidemiological sub-group; CHAMPS team. 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