{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:31:31Z","timestamp":1784817091148,"version":"3.55.0"},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND In previous studies, we identified a deficiency of \u03b3\u03b4 T cells and an increased prevalence of anti-Anisakis simplex antibodies in patients with Crohn\u2019s disease (CD). Additionally, decreased gene expression of the interleukin 2 (IL-2) receptor \u03b3 subunit (CD132) was observed in tissues from CD patients.  OBJECTIVE To analyse the gene expression of IL-7 and its receptors in tissues from CD patients and to explore its relationship with anti-A. simplex antibodies.  METHODS 52 patients diagnosed with CD were compared with a control group of 52 healthy individuals. Peripheral blood samples were analysed to assess levels of anti-A. simplex antibodies and IL-7. In addition, intestinal tissue samples from 20 subjects in each group were examined to evaluate IL-7 gene expression, IL-7 protein levels, the IL-2 receptor \u03b3 subunit (CD132), the IL-7 receptor \u03b1 subunit (CD127), and caspase-3 expression.  FINDINGS Anti-A. simplex antibody levels were elevated in patients with CD. Caspase-3 expression was significantly reduced in the tissues of CD patients with anti-A. simplex IgA, and this reduction extended to IgG and IgE in healthy individuals. A negative correlation was observed between caspase-3 levels and serum anti-A. simplex IgA, as well as IL-7 levels in the tissues of CD patients. In healthy subjects, tissue IL-7 levels were lower in those positive for anti-A. simplex IgA, while serum IL-7 levels were higher in individuals positive for anti-A. simplex IgG.  MAIN CONCLUSIONS This study revealed the interplay between IL-7 signalling, \u03b3\u03b4 T cell deficiency, and immune responses to A. simplex in CD. Our findings underscored a cause-effect relationship between CD132 deficiency, \u03b3\u03b4 T cell depletion, and defective mucosal immunity, which may drive both CD inflammation and susceptibility to parasitic infections like A. simplex.<\/jats:p>","DOI":"10.1590\/0074-02760250129","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T11:16:17Z","timestamp":1773054977000},"source":"Crossref","is-referenced-by-count":2,"title":["Immune dysregulation, apoptosis impairment, and enhanced seroreactivity to Anisakis simplex in Crohn\u2019s disease: interplay of IL-7\/IL-7R signalling and CD132 deficiency"],"prefix":"10.1590","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7948-9889","authenticated-orcid":false,"given":"Carmen","family":"Cu\u00e9llar","sequence":"first","affiliation":[{"name":"Complutense University of Madrid,  Spain; Complutense University of Madrid,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Hurtado-Marcos","sequence":"additional","affiliation":[{"name":"CEU Universities,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Valdivieso","sequence":"additional","affiliation":[{"name":"CEU Universities,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucianna","family":"Vaccaro","sequence":"additional","affiliation":[{"name":"CEU Universities,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Gonz\u00e1lez-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Complutense University of Madrid,  Spain; Complutense University of Madrid,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Isabel","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Arnau de Vilanova Hospital,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaume","family":"P\u00e9rez-Griera","sequence":"additional","affiliation":[{"name":"University Clinical Hospital,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Benlloch","sequence":"additional","affiliation":[{"name":"Arnau de Vilanova Hospital,  Spain; CEU Universities,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cirilo","family":"Amor\u00f3s","sequence":"additional","affiliation":[{"name":"Arnau de Vilanova Hospital,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Gil-Borr\u00e1s","sequence":"additional","affiliation":[{"name":"CEU Universities,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Sorando-Serra","sequence":"additional","affiliation":[{"name":"Arnau de Vilanova Hospital,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Cano-Cano","sequence":"additional","affiliation":[{"name":"Arnau de Vilanova Hospital,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Andreu-Ballester","sequence":"additional","affiliation":[{"name":"Complutense University of Madrid,  Spain; Foundation for the Promotion of Health and Biomedical Research in the Valencian Region,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"10432","key":"ref1","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/S0140-6736(23)02586-2","article-title":"Crohn\u2019s disease","volume":"403","author":"Dolinger M","year":"2024","journal-title":"Lancet"},{"issue":"9","key":"ref2","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.1007\/s10620-011-1636-8","article-title":"Deficit of gammadelta T lymphocytes in the peripheral blood of patients with Crohn\u2019s disease","volume":"56","author":"Andreu-Ballester JC","year":"2011","journal-title":"Dig Dis Sci"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1053\/j.gastro.2023.09.019","article-title":"Pathobionts in inflammatory bowel disease: origins, underlying mechanisms, and implications for clinical care","volume":"166","author":"Gilliland A","year":"2024","journal-title":"Gastroenterology"},{"issue":"3 Pt 1","key":"ref4","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/S0091-6749(99)70336-2","article-title":"Immunopathogenesis of human gastrointestinal infection by Anisakis simplex","volume":"104","author":"del Pozo V","year":"1999","journal-title":"J Allergy Clin Immunol"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1079\/JOH2001104","article-title":"Immunoglobulins anti-Anisakis simplex in patients with gastrointestinal diseases","volume":"76","author":"Gutierrez R","year":"2002","journal-title":"J Helminthol"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.prp.2014.06.022","article-title":"Intestinal anisakidosis: histopathological findings and differential diagnosis","volume":"210","author":"Baron L","year":"2014","journal-title":"Pathol Res Pract"},{"issue":"8","key":"ref7","article-title":"Prevalence of anti-anisakis simplex antibodies in a cohort of patients with inflammatory bowel disease in Norway","volume":"14","author":"de la Hoz-Martin MP","year":"2025","journal-title":"Pathogens"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.ijppaw.2019.04.007","article-title":"Anisakis nematodes in fish and shellfish- from infection to allergies","volume":"9","author":"Aibinu IE","year":"2019","journal-title":"Int J Parasitol Parasites 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L","year":"2019","journal-title":"J Clin Invest"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1159\/000007668","article-title":"Anti-anisakis antibodies in the clinical course of Crohn\u2019s disease","volume":"60","author":"Guillen-Bueno R","year":"1999","journal-title":"Digestion"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3109\/00365528909091339","article-title":"Classification of inflammatory bowel disease","volume":"170","author":"Lennard-Jones JE","year":"1989","journal-title":"Scand J Gastroenterol Suppl"},{"issue":"8","key":"ref14","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.crohns.2013.02.005","article-title":"Optimising monitoring in the management of Crohn\u2019s disease: a physician\u2019s perspective","volume":"7","author":"Papay P","year":"2013","journal-title":"J Crohns 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for myeloid cell homeostasis and reconstitution by hematopoietic stem cells","volume":"90","author":"Cool T","year":"2020","journal-title":"Exp Hematol"},{"issue":"14","key":"ref19","doi-asserted-by":"crossref","first-page":"7172","DOI":"10.1073\/pnas.93.14.7172","article-title":"Interleukin 7 receptor-deficient mice lack gammadelta T cells","volume":"93","author":"Maki K","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"issue":"105","key":"ref20","article-title":"Dysregulation of gammadelta intraepithelial lymphocytes precedes Crohn\u2019s disease-like ileitis","volume":"10","author":"Xu W","year":"2025","journal-title":"Sci Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1159\/000051911","article-title":"Existence of variant gamma delta T cells in Crohn\u2019s disease","volume":"63","author":"Ishiguro Y","year":"2001","journal-title":"Digestion"},{"issue":"8","key":"ref22","article-title":"Apoptotic caspase inhibits innate immune signaling 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