{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:46:08Z","timestamp":1772819168587,"version":"3.50.1"},"reference-count":70,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2012\/16525-2"],"award-info":[{"award-number":["2012\/16525-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2017\/18611-7"],"award-info":[{"award-number":["2017\/18611-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2013\/20509-5"],"award-info":[{"award-number":["2013\/20509-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["431403\/2016-3"],"award-info":[{"award-number":["431403\/2016-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["2012\/16525-2"],"award-info":[{"award-number":["2012\/16525-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["2017\/18611-7"],"award-info":[{"award-number":["2017\/18611-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["2013\/20509-5"],"award-info":[{"award-number":["2013\/20509-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["431403\/2016-3"],"award-info":[{"award-number":["431403\/2016-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND Plasmodium vivax rosetting is a cytoadhesion phenomenon associated with parasite virulence and clinical manifestations of malaria. However, the molecular mechanisms underlying this process remain poorly understood. Comparative transcriptomic analysis between isolates with different rosetting capacities may provide insights into the molecular base and clinical outcome of parasite populations with distinct rosetting characteristics.  OBJECTIVES Our study aims to identify and describe the transcription profile of P. vivax isolates with high and low rosetting rates.  METHODS We used RNA-seq to compare the transcriptomes of 10 field P. vivax isolates from the Brazilian Amazon.  FINDINGS Among the 492 differentially expressed genes of P. vivax isolates with high rosetting (HR) versus low rosetting (LR) formation, 172 (34,96%) are annotated as genes conserved within Plasmodium and of unknown function. The expression profiles of the other 320 genes (65,04%) highlight the importance of integral membrane proteins and membrane-associated proteins with adhesive or adhesin-like properties, representing 10% of the transcribed genes (53 genes), such as Plasmodium Helical Interspersed Sub-telomeric (PHIST) proteins in rosetting phenotypes. Transcriptomic analyses revealed that approximately 4% (19 genes) of differentially expressed genes were kinases and 50% (248 genes) other proteins. Among cell surface proteins and integral\/membrane-associated proteins, differentiated expression and positive regulation of representative 6-cysteine gene family were observed in HR formation group, which includes a tryptophan-rich protein (TRAG16), the 41K blood stage antigen precursor 41-3 protein, and merozoite surface protein 7-like (MSP7-like).  MAIN CONCLUSIONS These results contribute to understanding the molecular basis of P. vivax rosetting.<\/jats:p>","DOI":"10.1590\/0074-02760250198","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:36:26Z","timestamp":1772796986000},"source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomics of Plasmodium vivax rosetting"],"prefix":"10.1590","volume":"121","author":[{"given":"Catarina","family":"Bourgard","sequence":"first","affiliation":[{"name":"Universidade de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia Weber","family":"Ferraboli","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie Costa Pinto","family":"Lopes","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o de Medicina Tropical Dr Heitor Vieira Dourado,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus Vinicius Guimar\u00e3es de","family":"Lacerda","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil; Funda\u00e7\u00e3o de Medicina Tropical Dr Heitor Vieira Dourado,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Sunnerhagen","sequence":"additional","affiliation":[{"name":"University of Gothenburg,  Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6406-2057","authenticated-orcid":false,"given":"Letusa","family":"Albrecht","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9969-7300","authenticated-orcid":false,"given":"Fabio Trindade Maranh\u00e3o","family":"Costa","sequence":"additional","affiliation":[{"name":"Universidade de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"8","key":"ref1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0000774","article-title":"The international limits and population at risk of Plasmodium vivax transmission in 2009","volume":"4","author":"Guerra CA","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1128\/CMR.00008-09","article-title":"Resistance to therapies for infection by Plasmodium vivax","volume":"22","author":"Baird JK","year":"2009","journal-title":"Clin Microbiol Rev"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.0050128","article-title":"Multidrug-resistant Plasmodium vivax associated with severe and fatal malaria: a prospective study in Papua, Indonesia","volume":"5","author":"Tjitra E","year":"2008","journal-title":"PLoS Med"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1097\/QCO.0b013e32832f14c1","article-title":"New developments in Plasmodium vivax malaria: severe disease and the rise of chloroquine resistance","volume":"22","author":"Price RN","year":"2009","journal-title":"Curr Opin Infect Dis"},{"issue":"9","key":"ref5","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.micinf.2012.02.011","article-title":"Naturally acquired antibodies to Plasmodium vivax blood-stage vaccine candidates (PvMSP-119 and PvMSP-3\u03b1359-798) and their relationship with hematological features in malaria patients from the Brazilian Amazon","volume":"14","author":"Mour\u00e3o LC","year":"2012","journal-title":"Microbes Infect"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1475-2875-11-12","article-title":"Understanding the clinical spectrum of complicated Plasmodium vivax malaria: a systematic review on the contributions of the Brazilian literature","volume":"11","author":"Lacerda MV","year":"2012","journal-title":"Malar J"},{"issue":"7","key":"ref7","doi-asserted-by":"crossref","first-page":"2511","DOI":"10.1073\/pnas.87.7.2511","article-title":"Antibodies to a histidine-rich protein (PfHRP1) disrupt spontaneously formed Plasmodium falciparum erythrocyte rosettes","volume":"87","author":"Carlson J","year":"1990","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"289","DOI":"10.4269\/ajtmh.1988.38.289","article-title":"Rosetting: a new cytoadherence property of malaria-infected erythrocytes","volume":"38","author":"Leech JH","year":"1988","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0169-4758(89)90141-5","article-title":"Why do Plasmodium falciparumm-infected erythrocytes form spontaneous erythrocyte rosettes?","volume":"5","author":"Wahlgren M","year":"1989","journal-title":"Parasitol Today"},{"issue":"4254","key":"ref10","first-page":"673","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"Trager W","year":"1976","journal-title":"Science (1979)"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1084\/jem.187.1.15","article-title":"Identification of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) as the rosetting ligand of the malaria parasite P. falciparum","volume":"187","author":"Chen Q","year":"1998","journal-title":"J Exp Med"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/nm.3812","article-title":"RIFINs are adhesins implicated in severe Plasmodium falciparum malaria","volume":"21","author":"Goel S","year":"2015","journal-title":"Nat Med"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.chom.2014.06.004","article-title":"STEVOR Is a Plasmodium falciparum erythrocyte binding protein that mediates merozoite invasion and rosetting","volume":"16","author":"Niang M","year":"2014","journal-title":"Cell Host Microbe"},{"issue":"6639","key":"ref14","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/40888","article-title":"P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1","volume":"388","author":"Rowe JA","year":"1997","journal-title":"Nature"},{"issue":"7","key":"ref15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002781","article-title":"Structural basis for the ABO blood-group dependence of Plasmodium falciparum rosetting","volume":"8","author":"Vigan-Womas I","year":"2012","journal-title":"PLoS Pathog"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1128\/CMR.13.3.439","article-title":"Molecular aspects of severe malaria","volume":"13","author":"Chen Q","year":"2000","journal-title":"Clin Microbiol Rev"},{"issue":"6","key":"ref17","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/0035-9203(95)90422-0","article-title":"Rosette formation by Plasmodium vivax","volume":"89","author":"Udomsangpetch R","year":"1995","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"13","key":"ref18","doi-asserted-by":"crossref","DOI":"10.1182\/blood-2011-04-348748","article-title":"A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax","volume":"118","author":"Russell B","year":"2011","journal-title":"Blood"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"73","DOI":"10.4269\/ajtmh.1998.59.73","article-title":"Characteristics of Plasmodium vivax-infected erythrocyte rosettes","volume":"59","author":"Chotivanich KT","year":"1998","journal-title":"Am J Trop Med Hyg"},{"issue":"18","key":"ref20","article-title":"Glycophorin C (CD236R) mediates vivax malaria parasite rosetting to normocytes","volume":"123","author":"Lee WC","year":"2014","journal-title":"Blood"},{"issue":"8","key":"ref21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004912","article-title":"Rheopathologic consequence of Plasmodium vivax rosette formation","volume":"10","author":"Zhang R","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010991","article-title":"Long read single cell RNA sequencing reveals the isoform diversity of Plasmodium vivax transcripts","volume":"16","author":"Hazzard B","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011926","article-title":"Comparative transcriptomics reveal differential gene expression among Plasmodium vivax geographical isolates and implications on erythrocyte invasion mechanisms","volume":"18","author":"Kepple D","year":"2024","journal-title":"PLoS Negl Trop Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1011692","DOI":"10.3389\/fcimb.2022.1011692","article-title":"Band 3-mediated Plasmodium vivax invasion is associated with transcriptional variation in PvTRAg genes","volume":"12","author":"De Meulenaere K","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"ref25","doi-asserted-by":"crossref","DOI":"10.1128\/msystems.00258-22","article-title":"Determination of the stage composition of Plasmodium infections from bulk gene expression data","volume":"7","author":"Tebben K","year":"2022","journal-title":"mSystems"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1186\/s12864-025-11752-1","article-title":"De novo assembly of Plasmodium interspersed repeat (pir) genes from Plasmodium vivax RNAseq data suggests geographic conservation of sub-family transcription","volume":"26","author":"Little TS","year":"2025","journal-title":"BMC Genomics"},{"key":"ref27","first-page":"3","article-title":"Vector incrimination and entomological inoculation rates","volume":"72","author":"Beier JC","year":"2002","journal-title":"Methods Mol Med"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/1475-2875-8-115","article-title":"Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood","volume":"8","author":"Sriprawat K","year":"2009","journal-title":"Malar J"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0053160","article-title":"Effective preparation of Plasmodium vivax field isolates for high-throughput whole genome sequencing","volume":"8","author":"Auburn S","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.pt.2007.09.010","article-title":"Cultivation of Plasmodium vivax","volume":"24","author":"Udomsangpetch R","year":"2008","journal-title":"Trends Parasitol"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"16706","DOI":"10.1038\/s41598-020-73713-w","article-title":"Rosettes integrity protects Plasmodium vivax of being phagocytized","volume":"10","author":"Albrecht L","year":"2020","journal-title":"Sci Rep"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1471-2164-8-45","article-title":"Patterns of gene recombination shape var gene repertoires in Plasmodium falciparum: comparisons of geographically diverse isolates","volume":"8","author":"Kraemer SM","year":"2007","journal-title":"BMC Genomics"},{"key":"ref33","series-title":"Methods in malaria research","first-page":"94","author":"Moll K","year":"2008","edition":"5th"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"5089","DOI":"10.1038\/s41598-021-84607-w","article-title":"A suitable RNA preparation methodology for whole transcriptome shotgun sequencing harvested from Plasmodium vivax-infected patients","volume":"11","author":"Bourgard C","year":"2021","journal-title":"Sci Rep"},{"issue":"W1","key":"ref35","doi-asserted-by":"crossref","first-page":"W345","DOI":"10.1093\/nar\/gkac247","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2022 update","volume":"50","author":"Afgan E","year":"2022","journal-title":"Nucleic Acids Res"},{"issue":"Database issue","key":"ref36","doi-asserted-by":"crossref","first-page":"D539","DOI":"10.1093\/nar\/gkn814","article-title":"PlasmoDB: a functional genomic database for malaria parasites","volume":"37","author":"Aurrecoechea C","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"4","DOI":"10.12688\/wellcomeopenres.9876.1","article-title":"A new Plasmodium vivax reference sequence with improved assembly of the subtelomeres reveals an abundance of pir genes","volume":"1","author":"Auburn S","year":"2016","journal-title":"Wellcome Open Res"},{"issue":"15","key":"ref38","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger AM","year":"2014","journal-title":"Bioinformatics"},{"issue":"9","key":"ref39","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1093\/bioinformatics\/btp120","article-title":"TopHat: discovering splice junctions with RNA-Seq","volume":"25","author":"Trapnell C","year":"2009","journal-title":"Bioinformatics"},{"issue":"2","key":"ref40","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq \u2014 a Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders S","year":"2015","journal-title":"Bioinformatics"},{"issue":"12","key":"ref41","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love MI","year":"2014","journal-title":"Genome Biol"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s40588-020-00155-3","article-title":"Insights on rosetting phenomenon in Plasmodium vivax malaria","volume":"8","author":"Bittencourt NC","year":"2021","journal-title":"Curr Clin Microbiol Rep"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1128\/iai.63.6.2323-2326.1995","article-title":"Plasmodium falciparum rosetting is associated with malaria severity in Kenya","volume":"63","author":"Rowe A","year":"1995","journal-title":"Infect Immun"},{"issue":"10","key":"ref44","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.pt.2022.08.001","article-title":"Evolving perspectives on rosetting in malaria","volume":"38","author":"Lee WC","year":"2022","journal-title":"Trends Parasitol"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4269\/ajtmh.1993.48.149","article-title":"The effects of hemoglobin genotype and ABO blood group on the formation of rosettes by Plasmodium falciparum-infected red blood cells","volume":"48","author":"Carlson J","year":"1993","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"ref46","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1084\/jem.176.5.1311","article-title":"Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions","volume":"176","author":"Carlson J","year":"1992","journal-title":"J Exp Med"},{"issue":"5","key":"ref47","doi-asserted-by":"crossref","first-page":"2971","DOI":"10.1128\/IAI.68.5.2971-2975.2000","article-title":"Blood group A antigen is a coreceptor in Plasmodium falciparum rosetting","volume":"68","author":"Barragan A","year":"2000","journal-title":"Infect Immun"},{"issue":"1","key":"ref48","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1475-2875-10-17","article-title":"var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2","volume":"10","author":"Albrecht L","year":"2011","journal-title":"Malar J"},{"issue":"1","key":"ref49","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1186\/1475-2875-10-180","article-title":"Design of a variant surface antigen-supplemented microarray chip for whole transcriptome analysis of multiple Plasmodium falciparum cytoadherent strains, and identification of strain-transcendent rif and stevor genes","volume":"10","author":"Claessens A","year":"2011","journal-title":"Malar J"},{"issue":"19","key":"ref50","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1177\/15353702231198070","article-title":"Plasmodium vivax transcriptomics: What is new?","volume":"248","author":"Ferraboli JW","year":"2023","journal-title":"Exp Biol Med"},{"issue":"8","key":"ref51","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010633","article-title":"Single-cell RNA sequencing of Plasmodium vivax sporozoites reveals stage- and species-specific transcriptomic signatures","volume":"16","author":"Ruberto AA","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"ref52","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.chom.2010.09.006","article-title":"The systematic functional analysis of Plasmodium protein kinases identifies essential regulators of mosquito transmission","volume":"8","author":"Tewari R","year":"2010","journal-title":"Cell Host Microbe"},{"issue":"20","key":"ref53","doi-asserted-by":"crossref","first-page":"3519","DOI":"10.4161\/cc.10.20.17791","article-title":"Molecular cloning and characterization of mitogen-activated protein kinase 2 in Toxoplasma gondii","volume":"10","author":"Huang H","year":"2011","journal-title":"Cell Cycle"},{"issue":"1","key":"ref54","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.molbiopara.2006.10.012","article-title":"Raf kinase inhibitor protein affects activity of Plasmodium falciparum calcium-dependent protein kinase 1","volume":"151","author":"Kugelstadt D","year":"2007","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"ref55","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/0006-291X(81)90466-6","article-title":"Intraerythrocytic plasmodial calcium metabolism","volume":"103","author":"Leida MN","year":"1981","journal-title":"Biochem Biophys Res Commun"},{"issue":"6","key":"ref56","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/0169-4758(90)90356-9","article-title":"Calcium metabolism in malaria-infected erythrocytes","volume":"6","author":"Krishna S","year":"1990","journal-title":"Parasitol Today"},{"issue":"1","key":"ref57","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0092-8674(01)00199-4","article-title":"A central role for P48\/45 in malaria parasite male gamete fertility","volume":"104","author":"van Dijk MR","year":"2001","journal-title":"Cell"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.mib.2017.11.029","article-title":"Plasmodium P47: a key gene for malaria transmission by mosquito vectors","volume":"40","author":"Molina-Cruz A","year":"2017","journal-title":"Curr Opin Microbiol"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.molbiopara.2017.04.005","article-title":"LCCL protein complex formation in Plasmodium is critically dependent on LAP1","volume":"214","author":"Tremp AZ","year":"2017","journal-title":"Mol Biochem Parasitol"},{"issue":"11","key":"ref60","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1084\/jem.20031274","article-title":"A multidomain adhesion protein family expressed in Plasmodium falciparum is essential for transmission to the mosquito","volume":"199","author":"Pradel G","year":"2004","journal-title":"J Exp Med"},{"issue":"10","key":"ref61","article-title":"Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development","volume":"13","author":"Schneider MP","year":"2017","journal-title":"PLoS Pathog"},{"issue":"1","key":"ref62","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ijpara.2017.08.015","article-title":"A map of the subcellular distribution of phosphoinositides in the erythrocytic cycle of the malaria parasite Plasmodium falciparum","volume":"48","author":"Ebrahimzadeh Z","year":"2018","journal-title":"Int J Parasitol"},{"issue":"5","key":"ref63","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0127500","article-title":"Characterization of Plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1","volume":"10","author":"Cheng Y","year":"2015","journal-title":"PLoS One"},{"issue":"1","key":"ref64","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1186\/s12936-015-0750-z","article-title":"P-selectin is a host receptor for Plasmodium MSP7 ligands","volume":"14","author":"Perrin AJ","year":"2015","journal-title":"Malar J"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.meegid.2017.01.024","article-title":"Evolution of the merozoite surface protein 7 (msp7 ) family in Plasmodium vivax and P. falciparum : a comparative approach","volume":"50","author":"Castillo AI","year":"2017","journal-title":"Infect Gen Evol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"811390","DOI":"10.3389\/fcimb.2021.811390","article-title":"Antigen discovery in circulating extracellular vesicles from Plasmodium vivax patients","volume":"11","author":"Aparici-Herraiz I","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"issue":"10","key":"ref67","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.tcb.2004.08.002","article-title":"The glideosome: a molecular machine powering motility and host-cell invasion by Apicomplexa","volume":"14","author":"Keeley A","year":"2004","journal-title":"Trends Cell Biol"},{"issue":"6","key":"ref68","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1086\/511309","article-title":"Characterization of a Plasmodium falciparum macrophage-migration inhibitory factor homologue","volume":"195","author":"Cordery DV","year":"2007","journal-title":"J Infect Dis"},{"issue":"W1","key":"ref69","doi-asserted-by":"crossref","first-page":"W147","DOI":"10.1093\/nar\/gkw419","article-title":"Heatmapper: web-enabled heat mapping for all","volume":"44","author":"Babicki S","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"W1","key":"ref70","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: a web tool for visualizing clustering of multivariate data using principal component analysis and heatmap","volume":"43","author":"Metsalu T","year":"2015","journal-title":"Nucleic Acids Res"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0074-02762026000105015&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:36:38Z","timestamp":1772796998000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762026000105015&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":70,"alternative-id":["S0074-02762026000105015"],"URL":"https:\/\/doi.org\/10.1590\/0074-02760250198","relation":{},"ISSN":["1678-8060","0074-0276"],"issn-type":[{"value":"1678-8060","type":"electronic"},{"value":"0074-0276","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e250198"}}