{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:17:31Z","timestamp":1787023051023,"version":"build-2736575974"},"reference-count":74,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010253","name":"UBACYT","doi-asserted-by":"crossref","award":["20020220300118BA"],"award-info":[{"award-number":["20020220300118BA"]}],"id":[{"id":"10.13039\/501100010253","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND Chagas disease (CD) and Leishmaniasis, caused by Trypanosoma cruzi and Leishmania species, are treated with outdated drugs that are toxic and have limited efficacy. New therapeutic alternatives are therefore needed. Natural products are valuable scaffolds for drug discovery, and Asteraceae species exhibit microbicidal activity.  OBJECTIVES This study evaluated the antiparasitic activity and selectivity of two Asteraceae species, Chromolaena hookeriana and Campuloclinium macrocephalum, against trypanosomatids.  METHODS C. hookeriana and C. macrocephalum extracts were profiled by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS\/MS), and their cytotoxicity and antiparasitic activity were evaluated against multiple forms and strains of T. cruzi and Leishmania amazonensis (L. amazonensis) in 2D and 3D cultures.  FINDINGS UHPLC-MS\/MS revealed predominantly pentacyclic triterpenoids in C. macrocephalum, including ursolic and oleanolic acids (negative mode) and lupeol derivatives (positive mode). C. macrocephalum was more effective than Benznidazole (Bz) (EC50 = 1.17 vs 4.47 \u00b5g\/mL) against epimastigotes and nine times more potent against trypomastigotes (EC50 = 0.38 vs 3.5 \u00b5g\/mL). Both extracts matched Bz activity on intracellular T. cruzi but exhibited time-dependent cytotoxicity. They also showed similar effectiveness to miltefosine (Mt) against L. amazonensis amastigotes (EC50 = 0.36\u20130.70 \u00b5g\/mL).  MAIN CONCLUSIONS The chemical profile supports the stronger antiparasitic activity of C. macrocephalum, likely linked to triterpenoids, and highlights Asteraceae extracts as promising candidates for drug discovery against neglected tropical diseases (NTDs).<\/jats:p>","DOI":"10.1590\/0074-02760250296","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:29:23Z","timestamp":1778498963000},"source":"Crossref","is-referenced-by-count":1,"title":["In vitro anti-Trypanosomatid activity and chemical profile of crude extracts of Chromolaena hookeriana and Campuloclinium macrocephalum (Asteraceae)"],"prefix":"10.1590","volume":"121","author":[{"given":"Ludmila Ferreira de Almeida","family":"Fiuza","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketlym","family":"da Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krislayne","family":"Nunes","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldana Malen","family":"Corlatti","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas - Universidad de Buenos Aires,  Argentina; Universidad de Buenos Aires,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Cecilia","family":"Laurella","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas - Universidad de Buenos Aires,  Argentina; Universidad de Buenos Aires,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simony Carvalho","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendo Araujo","family":"Gomes","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana Guimar\u00e3es","family":"Leit\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9322-5748","authenticated-orcid":false,"given":"Valeria Patricia","family":"S\u00fclsen","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas - Universidad de Buenos Aires,  Argentina; Universidad de Buenos Aires,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-6106","authenticated-orcid":false,"given":"Maria de Nazar\u00e9 Correia","family":"Soeiro","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","article-title":"Neglected tropical diseases in children: an assessment of gaps in research prioritization","volume":"13","author":"Rees CA","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s40249-018-0444-1","article-title":"Innovation in neglected tropical disease drug discovery and development","volume":"7","author":"Weng HB","year":"2018","journal-title":"Infect Dis 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