{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:56:44Z","timestamp":1781265404067,"version":"3.54.1"},"reference-count":38,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNPq\/CAPES\/FAPEMIG \u2013 INCT-Teranano","award":["CNPq: 403193\/2022-2"],"award-info":[{"award-number":["CNPq: 403193\/2022-2"]}]},{"name":"CNPq\/CAPES\/FAPEMIG \u2013 INCT-Teranano","award":["FAPEMIG: APQ-03613-17"],"award-info":[{"award-number":["FAPEMIG: APQ-03613-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"abstract":"<jats:p>BACKGROUND Hepatitis delta virus (HDV) is associated with the worst prognosis among viral hepatitis infections; however, it remains largely underdiagnosed, particularly in endemic developing regions, underscoring the need for new, accessible serological methods for large-scale screening.  OBJECTIVES To develop HDV-mimetic molecules using phage display for application in immunodiagnostic platforms.  METHODS HDV-mimetic peptides were selected via phage display biopanning, sequenced and screened by phage-enzyme-linked immunosorbent assay (ELISA). Based on the sequences of these peptides, a recombinant protein (rHDV) was constructed and employed in an ELISA. The test was validated using 87 anti-HDV-positive samples and 93 hepatitis B virus (HBV) control samples collected from a public diagnostic laboratory in the Amazon region. Statistical analyses were performed to evaluate the diagnostic performance of the synthetic peptides and the recombinant protein.  FINDINGS Overall, rHDV exhibits a sensitivity of 74.71%, specificity of 97.85%, and area under the curve (AUC) of 0.8906. In HDV ribonucleic acid (HDV RNA)-positive patients, diagnostic performance improved, with a sensitivity of 88.0%, specificity of 98.92%, and AUC of 0.96.  MAIN CONCLUSIONS These findings highlight that the rHDV protein used in ELISA effectively discriminated HDV-infected individuals from patients monoinfected with hepatitis B. This demonstrates the potential of rHDV as an effective, rapid, and low-cost tool for HDV detection for broader HDV screening.<\/jats:p>","DOI":"10.1590\/0074-02760250343","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:06:05Z","timestamp":1781262365000},"source":"Crossref","is-referenced-by-count":0,"title":["Novel epitope-based diagnostic probes selected by phage display for the serological detection of HDV"],"prefix":"10.1590","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9383-7106","authenticated-orcid":false,"given":"Mariana Ara\u00fajo","family":"Costa","sequence":"first","affiliation":[{"name":"Universidade Federal de Uberl\u00e2ndia,  Brasil; Universidade Federal de Uberl\u00e2ndia,  Brasil; Secretaria de Sa\u00fade do Estado do Acre,  Brasil; Universidade Federal do Acre,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayany Cristina","family":"de Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal de Uberl\u00e2ndia,  Brasil; Universidade Federal de Uberl\u00e2ndia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e1rcio Peixoto","family":"Roca","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brasil; Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrhyan","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz-Fiocruz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Fellype Alves","family":"de Souza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hospital Estadual do Acre,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutilene Barbosa","family":"Souza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hospital Estadual do Acre,  Brasil; Universidade Federal da Bahia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela","family":"Sabatini","sequence":"additional","affiliation":[{"name":"Secretaria de Sa\u00fade do Estado do Acre,  Brasil; 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