{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:55:57Z","timestamp":1792032957366,"version":"4.4.0"},"reference-count":48,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"published-print":{"date-parts":[[2014,9]]},"DOI":"10.1590\/0074-0276130542","type":"journal-article","created":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T21:29:02Z","timestamp":1409174942000},"page":"805-813","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients"],"prefix":"10.1590","volume":"109","author":[{"given":"Juliana Maria Azevedo de","family":"Lyra","sequence":"first","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalh\u00e3es-Fiocruz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Maruza","sequence":"additional","affiliation":[{"name":"Secretaria do Estado de Pernambuco, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirela","family":"Verza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Estadual de Produ\u00e7\u00e3o e Pesquisa em Sa\u00fade, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Madileuza","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Secretaria do Estado de Pernambuco, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de F\u00e1tima Milit\u00e3o de","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalh\u00e3es-Fiocruz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L\u00facia","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Estadual de Produ\u00e7\u00e3o e Pesquisa em Sa\u00fade, Brasil; Universidade Luterana do Brasil, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Ximenes","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pernambuco, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cynthia","family":"Braga","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalh\u00e3es-Fiocruz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"Lucena-Silva","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalh\u00e3es-Fiocruz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","article-title":"The diagnostic performance of a single GeneXpert MTB\/RIF assay in an intensified tuberculosis case finding survey among HIV-infected prisoners in Malaysia","volume":"8","author":"Abed Al-Darraji HA","year":"2013","journal-title":"PLoS ONE"},{"key":"ref2","first-page":"855","article-title":"Yield of smear, culture and amplification tests from repeated sputum induction for the diagnosis of pulmonary tuberculosis","volume":"5","author":"Al Zahrani K","year":"2001","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1590\/S0036-46652014000200009","article-title":"Quantitative real-time PCR (q-PCR) for sputum smear diagnosis of pulmonary tuberculosis among people with HIV\/AIDS","volume":"56","author":"Albuquerque YM","year":"2014","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1007\/s100960000375","article-title":"Clinical evaluation of an in-house IS6110 polymerase chain reaction for diagnosis of tuberculosis","volume":"19","author":"Almeda J","year":"2000","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/JCM.38.1.345-350.2000","article-title":"Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR","volume":"38","author":"Al-Soud WA","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1128\/JCM.39.2.485-493.2001","article-title":"Purification and characterization of PCR-inhibitory components in blood cells","volume":"39","author":"Al-Soud WA","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1128\/JCM.02157-10","article-title":"Comparison of the Xpert MTB\/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and non-respiratory specimens","volume":"49","author":"Armand S","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.5588\/ijtld.12.0156","article-title":"Rapid molecular detection of pulmonary tuberculosis in HIV-infected patients in Santiago, Chile","volume":"16","author":"Balcells ME","year":"2012","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/S0140-6736(11)60438-8","article-title":"Feasibility, diagnostic accuracy and effectiveness of decentralised use of the Xpert MTB\/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study","volume":"377","author":"Boehme CC","year":"2011","journal-title":"Lancet"},{"key":"ref10","first-page":"281","article-title":"Problems in the standardization of the polymerase chain reaction for the diagnosis of pulmonary tuberculosis","volume":"33","author":"Bollela VR","year":"1999","journal-title":"Rev Saude Publica"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1097\/00006454-199905000-00008","article-title":"Clinical presentation of tuberculosis in culture-positive children. Pediatric Tuberculosis Consortium","volume":"18","author":"Burroughs M","year":"1999","journal-title":"Pediatr Infect Dis J"},{"key":"ref12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0044626","article-title":"A diagnostic accuracy study of Xpert\u00aeMTB\/RIF in HIV-positive patients with high clinical suspicion of pulmonary tuberculosis in Lima, Peru","volume":"7","author":"Carriquiry G","year":"2012","journal-title":"PLoS ONE"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"4357","DOI":"10.1128\/JCM.43.9.4357-4362.2005","article-title":"Diagnosis of extrapulmonary tuberculosis by smear, culture and PCR using universal sample processing technology","volume":"43","author":"Chakravorty S","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1054\/tuld.2000.0243","article-title":"Immunologic diagnosis of tuberculosis: a review","volume":"80","author":"Chan ED","year":"2000","journal-title":"Tuber Lung Dis"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0016321","article-title":"Nucleic acid amplification tests for diagnosis of smear-negative TB in a high HIV-prevalence setting: a prospective cohort study","volume":"6","author":"Davis JL","year":"2011","journal-title":"PLoS ONE"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1128\/JCM.00330-13","article-title":"Evaluation of Xpert MTB\/RIF for detection of tuberculosis from blood samples of HIV-infected adults confirms Mycobacterium tuberculosis bacteremia as an indicator of poor prognosis","volume":"51","author":"Feasey NA","year":"2013","journal-title":"J Clin Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/j.tube.2011.10.009","article-title":"Rapid DNA extraction for specific detection and quantitation of Mycobacterium tuberculosis DNA in sputum specimens using Taqman assays","volume":"91","author":"Gomez DI","year":"2011","journal-title":"Tuberculosis (Edinb)"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3604","DOI":"10.1128\/JCM.01654-12","article-title":"Rapid detection of the Mycobacterium tuberculosis complex by use of quenching probe PCR (geneCube)","volume":"50","author":"Hida Y","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.5588\/ijtld.13.0149","article-title":"Characterisation of Mycobacterium tuberculosis isolates lacking IS6110 in Vietnam","volume":"17","author":"Huyen MN","year":"2013","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1128\/JCM.42.3.1012-1015.2004","article-title":"Comparison of PCR with the routine procedure for diagnosis of tuberculosis in a population with high prevalences of tuberculosis and human immunodeficiency virus","volume":"42","author":"Kivihya-Ndugga L","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref21","article-title":"Screening for HIV-associated tuberculosis and rifampicin resistance before antiretroviral therapy using the Xpert MTB\/RIF assay: a prospective study","volume":"8","author":"Lawn SD","year":"2011","journal-title":"PLoS Med"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S1473-3099(13)70008-2","article-title":"Advances in tuberculosis diagnostics: the Xpert MTB\/RIF assay and future prospects for a point-of-care test","volume":"13","author":"Lawn SD","year":"2013","journal-title":"Lancet Infect Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1111\/jam.12119","article-title":"Evaluation of a IS6110-Taqman real-time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB","volume":"114","author":"Lira LA","year":"2013","journal-title":"J Appl Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1111\/j.1365-2958.1990.tb02073.x","article-title":"Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family","volume":"4","author":"McAdam RA","year":"1990","journal-title":"Mol Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1590\/S0074-02762011000200013","article-title":"Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection","volume":"106","author":"Michelon CT","year":"2011","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref26","first-page":"522","article-title":"Tuberculosis diagnosis in resource-limited settings: clinical use of GeneXpert in the diagnosis of smear-negative PTB: a case report","volume":"13","author":"Nakiyingi L","year":"2013","journal-title":"Afr Health Sci"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1080\/09674845.2012.12069146","article-title":"Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods","volume":"69","author":"O\u2019Donnell N","year":"2012","journal-title":"Br J Biomed Sci"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1093\/cid\/cis631","article-title":"Evaluation of the Xpert MTB\/RIF assay at a tertiary care referral hospital in a setting where tuberculosis and HIV infection are highly endemic","volume":"55","author":"O\u2019Grady J","year":"2012","journal-title":"Clin Infect Dis"},{"key":"ref29","article-title":"Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis","volume":"4","author":"Pai M","year":"2004","journal-title":"BMC Infect Dis"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"384","DOI":"10.5588\/ijtld.11.0276","article-title":"Impact of the Gen-Probe Amplified MTD<sup>\u00ae<\/sup> Test on tuberculosis diagnosis in children","volume":"16","author":"Papaventsis D","year":"2012","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3225","DOI":"10.1128\/JCM.01335-13","article-title":"Comparison of the Xpert MTB\/RIF and COBAS TaqMan MTB assays for detection of Mycobacterium tuberculosis in respiratory specimens","volume":"51","author":"Park KS","year":"2013","journal-title":"J Clin Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0039966","article-title":"The diagnostic accuracy of urine-based Xpert MTB\/RIF in HIV-infected hospitalized patients who are smear-negative or sputum scarce","volume":"7","author":"Peter JG","year":"2012","journal-title":"PLoS ONE"},{"key":"ref33","first-page":"361","article-title":"Rapid and efficient detection of extra-pulmonary Mycobacterium tuberculosis by PCR analysis","volume":"4","author":"Portillo-G\u00f3mez L","year":"2000","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0088626","article-title":"A multi-site validation in India of the line probe assay for the rapid diagnosis of multi-drug resistant tuberculosis directly from sputum specimens","volume":"9","author":"Raizada N","year":"2014","journal-title":"PLoS ONE"},{"key":"ref35","first-page":"S689","article-title":"Molecular diagnosis of tuberculosis","author":"Richeldi L","year":"1995","journal-title":"Eur Respir J"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.diagmicrobio.2012.08.007","article-title":"Comparison of the MGIT TBc immunochromatographic assay with the Accuprobe Gen-Probe TB assay for identification of Mycobacterium tuberculosis complex: results from a low-burden tuberculosis setting","volume":"74","author":"Roberts SA","year":"2012","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1128\/JCM.41.7.3233-3240.2003","article-title":"Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis","volume":"41","author":"Sarmiento OL","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2466-11-15","article-title":"Comparison of two laboratory-developed PCR methods for the diagnosis of pulmonary tuberculosis in Brazilian patients with and without HIV infection","volume":"11","author":"Scherer LC","year":"2011","journal-title":"BMC Pulm Med"},{"key":"ref39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1001061","article-title":"Comparison of Xpert MTB\/RIF with other nucleic acid technologies for diagnosing pulmonary tuberculosis in a high HIV prevalence setting: a prospective study","volume":"8","author":"Scott LE","year":"2011","journal-title":"PLoS Med"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1590\/S1517-83822012000100030","article-title":"Diagnosis of the pulmonary tuberculosis by polymerase chain reaction: a comparative study between HIV-positive and HIV-negative individuals","volume":"43","author":"Silva MR","year":"2012","journal-title":"Braz J Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1542\/peds.97.2.155","article-title":"Detection of Mycobacterium tuberculosis in clinical specimens from children using a polymerase chain reaction","volume":"97","author":"Smith KC","year":"1996","journal-title":"Pediatrics"},{"key":"ref42","first-page":"312","article-title":"Detection of Mycobacterium tuberculosis by a polymerase chain reaction colorimetric dot-blot assay","volume":"8","author":"Sperhacke RD","year":"2004","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.1128\/JCM.00411-09","article-title":"Direct detection of mycobacterial species in pulmonary specimens by two rapid amplification tests, the Gen-Probe amplified Mycobacterium tuberculosis direct test and the genotype mycobacteria direct test","volume":"47","author":"Syre H","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1136\/thoraxjnl-2013-203485","article-title":"Accuracy and impact of Xpert MTB\/RIF for the diagnosis of smear-negative or sputum-scarce tuberculosis using bronchoalveolar lavage fluid","volume":"68","author":"Theron G","year":"2013","journal-title":"Thorax"},{"key":"ref45","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/18.1.188","article-title":"IS6110, an IS-like element of Mycobacterium tuberculosis complex","volume":"18","author":"Thierry D","year":"1990","journal-title":"Nucleic Acids Res"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"2699","DOI":"10.1128\/JCM.33.10.2699-2703.1995","article-title":"Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis","volume":"33","author":"Vlaspolder F1","year":"1995","journal-title":"J Clin Microbiol"},{"key":"ref47","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2334-13-507","article-title":"Meta-analysis to compare the accuracy of GeneXpert, MODS and the WHO 2007 algorithm for diagnosis of smear-negative pulmonary tuberculosis","volume":"13","author":"Walusimbi S","year":"2013","journal-title":"BMC Infect Dis"},{"key":"ref48","year":"2008","journal-title":"Global tuberculosis control - surveillance, planning, financing"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/mioc\/v109n6\/0074-0276-mioc-0074-0276140542.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T19:01:44Z","timestamp":1650049304000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762014000600805&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["S0074-02762014000600805"],"URL":"https:\/\/doi.org\/10.1590\/0074-0276130542","relation":{},"ISSN":["0074-0276"],"issn-type":[{"value":"0074-0276","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]}}}