{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:00:12Z","timestamp":1778864412631,"version":"3.51.4"},"reference-count":46,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mem. Inst. Oswaldo Cruz"],"published-print":{"date-parts":[[2014,7]]},"DOI":"10.1590\/0074-0276140079","type":"journal-article","created":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T09:19:31Z","timestamp":1406539171000},"page":"506-508","source":"Crossref","is-referenced-by-count":17,"title":["Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus"],"prefix":"10.1590","volume":"109","author":[{"given":"S\u00e9rgio Luiz Bessa","family":"Luz","sequence":"first","affiliation":[{"name":"Instituto Le\u00f4nidas e Maria Deane-Fiocruz, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James Lee","family":"Crainey","sequence":"additional","affiliation":[{"name":"Instituto Le\u00f4nidas e Maria Deane-Fiocruz, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony John","family":"Shelley","sequence":"additional","affiliation":[{"name":"The Natural History Museum, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Rubio","sequence":"additional","affiliation":[{"name":"Instituto de Salud Carlos III, Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s00436-008-1164-4","article-title":"An atypical microfilaria in blood samples from inhabitants of Brazilian Amazon","volume":"104","author":"Adami YL","year":"2008","journal-title":"Parasitol Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1590\/0074-0276130243","article-title":"New records of Mansonella ozzardi: a parasite that is spreading from the state of Amazonas to previously uninfected areas of the state of Acre in the Purus River region","volume":"109","author":"Adami YL","year":"2014","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"198","DOI":"10.4269\/ajtmh.1993.48.198","article-title":"A sensitive serodiagnostic test for onchocerciasis using a cocktail of recombinant antigens","volume":"48","author":"Bradley JE","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"ref4","first-page":"454","article-title":"Specific detection of human antibodies to Onchocerca volvulus","volume":"40","author":"Cabrera Z","year":"1989","journal-title":"Ann Trop Med Parasitol"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1186\/1756-3305-6-289","article-title":"Interruption of Onchocerca volvulus transmission in northern Venezuela","volume":"6","author":"Convit J","year":"2013","journal-title":"Parasites Vectors"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"21","DOI":"10.2147\/RRTM.S30482","article-title":"Guide to detecting a potential recrudescence of onchocerciasis during the post treatment surveillance period: the American paradigm","volume":"3","author":"Cupp EW","year":"2012","journal-title":"Res Rep Trop Med"},{"key":"ref7","series-title":"Progress toward elimination of onchocerciasis in the Americas - 1993-2012. Morbidity and Mortality Weekly Report","author":"Eberhard M","year":"2013"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"334","DOI":"10.4269\/ajtmh.2007.77.334","article-title":"Elimination of Onchocercia volvulus transmission in the Santa Rosa focus of Guatemala","volume":"77","author":"Lindblade KA","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1086\/505081","article-title":"Field applicability of a rapid-format anti-Ov-16 antibody test for the assessment of onchocerciasis control measures in regions of endemicity","volume":"194","author":"Lipner EM","year":"2006","journal-title":"J Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1126\/science.2011741","article-title":"An immunogenic Onchocerca volvulus antigen: a specific and early marker of infection","volume":"251","author":"Lobos E","year":"1991","journal-title":"Science"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"491","DOI":"10.4269\/ajtmh.2012.11-0379","article-title":"Genetic characterization of atypical Mansonella (Mansonella) ozzardi microfilariae in human blood samples from north-eastern Peru","volume":"87","author":"Marcos LA","year":"2012","journal-title":"Am J Trop Med Hyg"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0020-7519(00)00156-9","article-title":"Characterisation of nuclear ribosomal DNA sequences from Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea) and development of a PCR-based method for their detection in skin biopsies","volume":"31","author":"Morales-Hojas R","year":"2001","journal-title":"Int J Parasitol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1017\/S003118200300324X","article-title":"The morphological discrimination of microfilariae of Onchocerca volvulus from Mansonella ozzardi","volume":"127","author":"Post RJ","year":"2003","journal-title":"Parasitology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/j.1755-0998.2008.02437.x","article-title":"Laser-assisted microdissection for the study of the ecology of parasites in their hosts","volume":"9","author":"Post RJ","year":"2009","journal-title":"Mol Ecol Resou"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002133","article-title":"Interruption of transmission of Onchocerca volvulus in the southern Chiapas focus, M\u00e9xico","volume":"7","author":"Rodr\u00edguez-P\u00e9rez MA","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0035-9203(01)90150-6","article-title":"The specificity of an ELISA for detection of Onchocerca volvulus in Brazil in an area endemic for Mansonella ozzardi","volume":"95","author":"Shelley AJ","year":"2001","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1590\/S0074-02762010000600016","article-title":"Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region","volume":"105","author":"Tang T-HT","year":"2010","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref18","series-title":"152nd Session of the Executive Committee","first-page":"33","volume-title":"Towards the elimination","year":"2013"},{"key":"ref19","series-title":"Estudo sobre a ocorr\u00eancia de infec\u00e7\u00f5es por fil\u00e1rias entre habitantes das comunidades ribeirinhas dos rios Antimary, Acre e Purus - regi\u00e3o de divisa leste do estado do Acre com sudoeste do estado do Amazonas","author":"Adami YL","year":"2009"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1590\/0074-0276130243","article-title":"New records of Mansonella ozzardi: a parasite that is spreading from the state of Amazonas to previously uninfected areas of the state of Acre in the Purus River region","volume":"109","author":"Adami YL","year":"2014","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1136\/jcp.2006.037093","article-title":"The role of a recombinant hybrid protein based ELISA for the serodiagnosis of Onchocerca volvulus","volume":"61","author":"Andrews JA","year":"2008","journal-title":"J Clin Pathol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1590\/S1726-46342012000100032","article-title":"Caracterizaci\u00f3n morfol\u00f3gica de microfilarias at\u00edpica y ozzardi del genero Mansonella","volume":"29","author":"Arrospide N","year":"2012","journal-title":"Rev Peru Med Exp Salud P\u00fablica"},{"key":"ref23","article-title":"Late onset onchocercal skin disease among Ethiopian immigrants","author":"Baum S","year":"2014","journal-title":"Br J Dermatol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"877","DOI":"10.4269\/ajtmh.1998.59.877","article-title":"A cocktail of recombinant Onchocerca volvulus antigens for serologic diagnosis with the potential to predict the endemicity of onchocerciasis infection","volume":"59","author":"Bradley JE","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"198","DOI":"10.4269\/ajtmh.1993.48.198","article-title":"A sensitive serodiagnostic test for onchocerciasis using a cocktail of recombinant antigens","volume":"48","author":"Bradley JE","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0065-308X(08)60219-5","article-title":"Molecular approaches to the diagnosis of onchocerciasis","volume":"37","author":"Bradley JE","year":"1996","journal-title":"Adv Parasitol"},{"key":"ref27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0000438","article-title":"A four-antigen mixture for rapid assessment of Onchocerca volvulus infection","volume":"3","author":"Burbelo PD","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"key":"ref28","doi-asserted-by":"crossref","DOI":"10.1186\/1756-3305-6-289","article-title":"Interruption of Onchocerca volvulus transmission in northern Venezuela","volume":"6","author":"Convit J","year":"2013","journal-title":"Parasites Vectors"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/j.1365-2249.1994.tb05504.x","article-title":"Evaluation of Onchocerca volvulus-specific IgG4 subclass serology as an index of onchocerciasis transmission potential of three Gabonese villages","volume":"98","author":"Egwang TG","year":"1994","journal-title":"Clinical Exp Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.actatropica.2013.02.016","article-title":"The disappearance of onchocerciasis from the Itwara focus, western Uganda, after elimination of the vector Simulium neavei and 19 years of annual ivermectin treatments","volume":"126","author":"Garms RTL","year":"2013","journal-title":"Acta Tropica"},{"key":"ref31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0069231","article-title":"Extended result reading in lateral flow tests detecting exposure to Onchocerca volvulus: a new technology to improve epidemiological surveillance tools","volume":"8","author":"Golden A","year":"2013","journal-title":"PLOS ONE"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1590\/S0074-02761997000200005","article-title":"Onchocerciasis in Ecuador: prevalence of infection on the Ecuador-Colombia border in the province of Esmeraldas","volume":"92","author":"Guderian JR","year":"1997","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref33","article-title":"Transmission of onchocerciasis in Wadelai focus of northwestern Uganda has been interrupted and the disease eliminated","author":"Katabarwa MN","year":"2014","journal-title":"J Parasitol Res"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/bjaceaccp\/mkn041","article-title":"Clinical tests: sensitivity and specificity","volume":"8","author":"Lalkhen AG","year":"2008","journal-title":"Contin Educ Anaesth Crit Care Pain"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1126\/science.2011741","article-title":"An immunogenic Onchocerca volvulus antigen: a specific and early marker of infection","volume":"251","author":"Lobos E","year":"1991","journal-title":"Science"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1590\/S0102-311X1991000400004","article-title":"Oncocercose entre \u00edndios Yanom\u00e1mi","volume":"7","author":"Moraes MAP","year":"1991","journal-title":"Cad Saude Publica"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0020-7519(00)00156-9","article-title":"Characterisation of nuclear ribosomal DNA sequences from Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea) and development of a PCR-based method for their detection in skin biopsies","volume":"31","author":"Morales-Hojas R","year":"2001","journal-title":"Int J Parasitol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0169-4758(93)90204-S","article-title":"Improved immunodiagnostic tests to monitor onchocerciasis control programmes - A multicenter effort","volume":"9","author":"Ramachandran CP","year":"1993","journal-title":"Parasitol Today"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/S0035-9203(03)80018-4","article-title":"Antibody detection for Onchocerca volvulus: comparison of the sensitivity of a cocktail of recombinant antigens used in the indirect enzyme linked immunosorbent assay with a rapid-format antibody card test","volume":"97","author":"Rodr\u00edguez-P\u00e9rez MA","year":"2003","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"147","DOI":"10.4269\/ajtmh.2008.78.147","article-title":"Evidence for supression of Onchocerca volvulus transmission in the Oaxaca focus in Mexico","volume":"78","author":"Rodr\u00edguez-P\u00e9rez MA","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/B978-0-12-391429-3.00008-3","article-title":"Assessment and monitoring of onchocerciasis in Latin America","volume":"77","author":"Rodr\u00edguez-P\u00e9rez MA","year":"2011","journal-title":"Adv Parasitol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1590\/S0102-311X2002000500009","article-title":"Human onchocerciasis in Brazil: an overview","volume":"18","author":"Shelley AJ","year":"2002","journal-title":"Cad Saude Publica"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0035-9203(01)90150-6","article-title":"The specificity of an ELISA for detection of Onchocerca volvulus in Brazil in an area endemic for Mansonella ozzardi","volume":"95","author":"Shelley AJ","year":"2001","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1590\/S0074-02762010000600016","article-title":"Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region","volume":"105","author":"Tang T-HT","year":"2010","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1136\/jcp.31.6.507","article-title":"Enzyme immunoassays with special reference to ELISA techniques","volume":"31","author":"Voller A","year":"1978","journal-title":"J Clin Pathol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1086\/317629","article-title":"A rapid-format antibody card test for diagnosis of onchocerciasis","volume":"182","author":"Weil GJ","year":"2000","journal-title":"J Infect Dis"}],"container-title":["Mem\u00f3rias do Instituto Oswaldo Cruz"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/mioc\/v109n4\/0074-0276-mioc-109-4-0506.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T03:37:53Z","timestamp":1565667473000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0074-02762014000400506&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["S0074-02762014000400506"],"URL":"https:\/\/doi.org\/10.1590\/0074-0276140079","relation":{},"ISSN":["0074-0276"],"issn-type":[{"value":"0074-0276","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7]]}}}