{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,24]],"date-time":"2025-05-24T22:23:12Z","timestamp":1748125392062},"reference-count":34,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Col. Bras. Cir."],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>Objective: Io evaluate the expression of p16INK4a and p53 biomarkers in conization specimens from patients with high grade cervical intraepithelial neoplasia (HG-CIN), correlating them with the ability to predict the recurrence. Methods : we conducted a retrospective study of patients with HG-CIN in cervical biopsy treated with conization between January 1999 and January 2006 who had a minimum follow-up of 18 months. The expression of the p16 and p53 was assessed by tissue microarrays and correlated with disease recurrence. For analysis, we used the test of proportions (chi-square), considering value p&lt;0.05, 95% CI and calculations of sensitivity, specificity and accuracy of these immunomarkers in predicting recurrence. Results : the series comprised 83 patients aged between 16 and 86 years (35\u00b111.7), divided into two groups: 30 with HG-CIN recurrence (study group) and 53 without recurrence (control group). Mean age, parity, smoking and conization technique were similar in both groups. The p53 expression was present in 43% of the study group and 57% of the control group, and the p16 was present in 43% of the study group and in 57% of the control group (p&gt;0.05). p53 had a positive predictive value (PPV) of 42% and negative predictive value (NPV) of 73%, sensitivity 70%, specificity of 47% and accuracy of 59%. The p16, PPV 42%, NPV 72%, sensitivity 66%, specificity of 49% and accuracy of 56%. Conclusion : immunohistochemistry expression of p53 and p16 showed low sensitivity and low specificity as predictors of HG-CIN recurrence after conization treatment.<\/jats:p>","DOI":"10.1590\/0100-69912016001008","type":"journal-article","created":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T22:19:00Z","timestamp":1460585940000},"page":"35-41","source":"Crossref","is-referenced-by-count":9,"title":["The role of P16ink4a and P53 immunostaining in predicting recurrence of HG-CIN after conization treatment"],"prefix":"10.1590","volume":"43","author":[{"given":"Fernanda Villar","family":"Fonseca","sequence":"first","affiliation":[{"name":"Hospital Erasto Gaertner, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio Daniel S.","family":"Tomasich","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Elizabeth","family":"Jung","sequence":"additional","affiliation":[{"name":"Hospital Erasto Gaertner, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Afonso","family":"Maestri","sequence":"additional","affiliation":[{"name":"Hospital Erasto Gaertner, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Newton S\u00e9rgio de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Cervical cancer","year":"2012"},{"key":"ref2","series-title":"C\u00e2ncer de colo uterino\/ taxas brutas de incid\u00eancia","year":"2012"},{"issue":"8","key":"ref3","first-page":"363","article-title":"M\u00e9todos diagn\u00f3sticos para o rastreamento do c\u00e2ncer de colo [editorial]","volume":"32","author":"Sarian LO","year":"2010","journal-title":"Rev Bras Ginecol 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