{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T12:00:53Z","timestamp":1648987253755},"reference-count":26,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ABCD, arq. bras. cir. dig."],"abstract":"<jats:p>ABSTRACT  Background: Researches on Chagas disease still use several animals and rats, due to size and susceptibility were preferred by many authors.  Aim:  To develop an experimental model of megacolon in rats inoculated with the strain Y of Trypanosoma cruzi.  Methods:  Thirty male Wistar rats were distributed in three groups inoculated with different inoculants: Group A: 600000, Group B: 1000000 and Group C: 1500000 blood trypomastigotes of T. cruzi. Animals were sedated intramuscularly at zero inoculation time (T0) and 60 days after inoculation (T60), to perform the barium enema in order to evaluate the dilatation of the different segments of colon in a comparative study of the measurements obtained, using a digital caliper. Evidence of infection was performed by blood smear collected from the animal\u2019s tail 18 days after inoculation with observation of blood forms.  Results:  Comparing the intestinal diameter of the inoculated animals with 60,0000 trypomastigotes in the T0 of infection with T60 days after the inoculation, significant dilatation was observed between the proximal, medial and distal segments (p&lt;0.01), indicating the establishment of the megacolon model. In addition, comparing intestinal diameter between the different segments, with in the T0 of infection and the T60 after inoculation, significant alterations were observed (p&lt;0.05).  Conclusion:  The proposed model was possible for in vivo studies of alterations due to infection by T. cruzi and functional alterations of the colon. In addition, the changes manifested in the colon are not directly proportional to the size of the inoculum, but to the time of infection that the animals were submitted, since the animals inoculated with 60,0000 blood forms were the ones which presented the most significant alterations.<\/jats:p>","DOI":"10.1590\/0102-672020180001e1341","type":"journal-article","created":{"date-parts":[[2018,3,3]],"date-time":"2018-03-03T23:11:13Z","timestamp":1520118673000},"source":"Crossref","is-referenced-by-count":1,"title":["RADIOLOGICAL STUDY OF MEGACOLON IN TRYPANOSOMA CRUZI INFECTED RATS"],"prefix":"10.1590","volume":"31","author":[{"given":"Carlos Edmundo Rodrigues","family":"FONTES","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Surgery,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula de","family":"ABREU","sequence":"additional","affiliation":[{"name":"State University of Maring\u00e1,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aretuza Zaupa","family":"GASPARIM","sequence":"additional","affiliation":[{"name":"State University of Maring\u00e1,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2018,3,1]]},"reference":[{"key":"ref1","series-title":"Boletim eletr\u00f4nico epidemiol\u00f3gico - situa\u00e7\u00e3o epidemiol\u00f3gica das zoonoses de interesse para a sa\u00fade p\u00fablica","year":"2010"},{"key":"ref2","first-page":"389","article-title":"Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi","volume":"4","author":"Brener Z","year":"1962","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1590\/S0074-02761909000200008","article-title":"Nova tripanosom\u00edase humana estudos sobre a morfologia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n.sp., agente etiol\u00f3gico de nova entidade m\u00f3rbida do homem","volume":"1","author":"Chagas C","year":"1909","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1590\/S0074-02762009000900006","article-title":"Epidemiology, control and surveillance of Chagas disease 100 years after its discovery","volume":"104","author":"Coura JR","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1590\/S0037-86822012000300002","article-title":"Chagas disease What is known and what should be improved: a systemic review","volume":"45","author":"Coura JR","year":"2012","journal-title":"Rev Soc Bras Med Trop"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1590\/S0102-311X1993000200012","article-title":"A doen\u00e7a de Chagas e seu controle na Am\u00e9rica Latina uma an\u00e1lise de possibilidades. 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Elsevier"}],"container-title":["ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S\u00e3o Paulo)"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/abcd\/v31n1\/0102-6720-abcd-31-01-e1341.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,3,10]],"date-time":"2018-03-10T19:03:56Z","timestamp":1520708636000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-67202018000100302&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,1]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018]]}},"alternative-id":["S0102-67202018000100302"],"URL":"https:\/\/doi.org\/10.1590\/0102-672020180001e1341","relation":{},"ISSN":["2317-6326","0102-6720"],"issn-type":[{"value":"2317-6326","type":"electronic"},{"value":"0102-6720","type":"print"}],"subject":[],"published":{"date-parts":[[2018,3,1]]}}}