{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:56:24Z","timestamp":1761663384167},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>This study aimed to evaluate the in vitro antifungal susceptibility of Candida species of head-and-neck-irradiated patients (Group 1), non-institutionalized (Group 2) and institutionalized elders (Group 3) using Etest(r) methodology. Candida was isolated from saliva and presumptively identified by CHROMagar Candida(r), confirmed by morphological criteria, carbohydrate assimilation (API 20C AUX(r)) and genetic typing (OPE 18). The collection was made from 29, 34 and 29 individuals (Groups 1, 2 and 3, respectively) with 67 isolates. Etest(r) strips (ketoconazole, itraconazole, fluconazole, amphotericin B and flucytosine) on RPMI (Roswell Park Memorial Institute) agar, on duplicate, were used to evaluate susceptibility. ATTC (American Type Culture Collection) 10231 (Candida albicans) was used as quality control. Among the 67 isolates of Candida species, most were susceptible to azoles, flucytosine and amphotericin B. None of the isolates showed resistance and dose-dependent susceptibility to amphotericin B. There were nine strains resistant to itraconazole, six to fluconazole and two to ketoconazole and ten dose-dependent, mainly to flucytocine. The highest MIC (minimum inhibitory concentration) to C. albicans, C. tropicalis, C. parapsilosis was 2.671 \u03bcg.mL-1, 8.104 \u03bcg.mL-1, 4.429 \u03bcg.mL-1, all for flucytosine. C. krusei and C. glabrata were associated with higher MIC for azoles and C. glabrata with higher MIC to flucytosine. In summary, susceptibility to all tested antifungal agents was evident. The isolates were more resistant to itraconazole and dose-dependent to flucytosine. A comparison of C. albicans in the three groups showed no outliers. Higher MIC was associated with C. krusei and C. glabrata.<\/jats:p>","DOI":"10.1590\/0103-6440201300115","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T11:36:12Z","timestamp":1427369772000},"page":"99-104","source":"Crossref","is-referenced-by-count":8,"title":["Antifungal Susceptibility In Vitro Determined by the Etest(r) for Candida Obtained from the Oral Cavity of Irradiated and Elderly Individuals"],"prefix":"10.1590","volume":"26","author":[{"given":"Edimilson Martins de","family":"Freitas","sequence":"first","affiliation":[{"name":"UNIMONTES - State University of Montes Claros, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Cavalcanti","family":"Monteiro","sequence":"additional","affiliation":[{"name":"UFPB - Federal University of Paraiba, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle Bonfim da Silva","family":"Fernandes","sequence":"additional","affiliation":[{"name":"UNIMONTES - State University of Montes Claros, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herc\u00edlio","family":"Martelli Junior","sequence":"additional","affiliation":[{"name":"UNIMONTES - State University of Montes Claros, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Rog\u00e9rio Ferreti","family":"Bonan","sequence":"additional","affiliation":[{"name":"UNIMONTES - State University of Montes Claros, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio Avelino Mota","family":"Nobre","sequence":"additional","affiliation":[{"name":"UNIMONTES - State University of Montes Claros, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s11046-008-9154-8","article-title":"Candida albicans and Candida tropicalis in oral candidosis: quantitative analysis, exoenzyme activity, and antifungal drug sensitivity","volume":"167","author":"Costa KRC","year":"2009","journal-title":"Mycopathol"},{"key":"ref2","series-title":"Int J Oral Sci","first-page":"156","volume-title":"Fluconazole susceptibility and genotypic heterogeneity of oral Candida albicans colonies from the patients with cancer receiving chemotherapy in China","volume":"1","author":"Sun J","year":"2009"},{"key":"ref3","first-page":"14","article-title":"Emerging fungal infections in immunocompromised patients","volume":"3","author":"Low CY","year":"2011","journal-title":"F1000 Medicine Reports"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4103\/0973-029X.92970","article-title":"Oropharyngeal candidosis relative frequency in radiotherapy patient for head and neck cancer","volume":"16","author":"Suryawanshi H","year":"2012","journal-title":"J Oral Maxillofac Pathol"},{"key":"ref5","series-title":"Oral tissue changes caused by radiation therapy and their management. 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