{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:55:22Z","timestamp":1782489322065,"version":"3.54.5"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>Studies have suggested that caffeine acts on bone promoting an increase of calcium excretion, inhibition of osteoblast proliferation and delay in tissue repair process, raising the risk of fractures, osteoporosis, periodontal disease and affecting the success of bone reconstructive procedures. The aim of this study was to analyze histomorphometrically the process of alveolar bone healing after tooth extraction in rats subjected to daily intake of boiled coffee or intraperitoneal administration of caffeine. Forty-five male rats were divided according to the treatment in Control group (C); Coffee group (CO) - treated with coffee since birth; and Caffeine (CAF) - intraperitoneal injection of aqueous solution of caffeine 1.5% (0.2 mL\/100g body weight) for 30 days. When weighing between 250-300 g they were anesthetized, subjected to extraction of the maxillary right incisor, and euthanized 7, 21 and 42 days after surgery for histological assessments of bone volume and the quality of formed bone in the dental socket. The qualitative results demonstrated larger amounts of blood clot and immature bone in animals under treatment of pure caffeine compared to coffee and control. Histometric analysis revealed that coffee treatment led to a 40% drop in bone formation, and caffeine a 60% drop in comparison to control animals (ANOVA p\u22640.01). It was concluded that both the daily ingestion of coffee and the intraperitoneal administration of caffeine in rats delayed the alveolar bone reparative process after tooth extraction, and this effect was more aggressive when pure caffeine was used.<\/jats:p>","DOI":"10.1590\/0103-6440201300219","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T07:39:11Z","timestamp":1427355551000},"page":"175-180","source":"Crossref","is-referenced-by-count":28,"title":["Effects of Coffee Intake and Intraperitoneal Caffeine on Bone Repair Process - A Histologic and Histometric Study"],"prefix":"10.1590","volume":"26","author":[{"given":"Rander Moreira","family":"Macedo","sequence":"first","affiliation":[{"name":"USP - University of S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Guilherme","family":"Brentegani","sequence":"additional","affiliation":[{"name":"USP - University of S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzie Aparecida de","family":"Lacerda","sequence":"additional","affiliation":[{"name":"USP - University of S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.3109\/00016357009028237","article-title":"Repair of the post-extraction alveolus in the Wistar rat: a histologic and autoradiographic study","volume":"28","author":"Johansen JR","year":"1970","journal-title":"Acta Odontol Scand"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1054\/ijom.2002.0292","article-title":"Early bone healing events in the human extraction sockets","volume":"31","author":"Devlin H","year":"2002","journal-title":"Int J Oral Maxilofac Surg"},{"key":"ref3","first-page":"1133","article-title":"Bone tissue formation in extraction sockets from sites with advanced periodontal disease: a histomorphometric study in humans","volume":"23","author":"Ahn JJ","year":"2008","journal-title":"Int J Oral Maxillofac Implants"},{"key":"ref4","first-page":"65","article-title":"Seventh International Caffeine. Workshop","volume":"32","author":"Barone JJ","year":"1994","journal-title":"Food Chem Toxicol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1093\/jn\/129.7.1361","article-title":"Teas and other beverages suppress D-galactosamine-induced liver injury in rats","volume":"129","author":"Sugiyama K","year":"1999","journal-title":"J Nutr"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1590\/S0103-64402010000300004","article-title":"Bone quality associated with daily intake of coffee: a biochemical, radiographic and histometric study","volume":"21","author":"Lacerda SA","year":"2010","journal-title":"Braz Dent J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1902\/jop.2012.120192","article-title":"Effects of estrogen deficiency and\/or caffeine intake on alveolar bone loss, density, and healing: a study in rats","volume":"84","author":"Bezerra JP","year":"2013","journal-title":"J Periodontol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1902\/jop.1999.70.3.283","article-title":"Combined effects of caffeine and prostaglandin E2 on the proliferation of osteoblast-like cells (UMR106-01)","volume":"70","author":"Kamagata-Kiyoura Y","year":"1999","journal-title":"J Periodont"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1097\/ID.0b013e31822b9b53","article-title":"Osseointegration of autogenous bone graft associated with osteoblastic cells under treatment with caffeine","volume":"20","author":"Macedo RM","year":"2011","journal-title":"Implant Dent"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1089\/jcr.2012.0027","article-title":"Effects of caffeine on bones of osteoporotic rats","volume":"2","author":"Macedo RM","year":"2012","journal-title":"Journal of Caffeine Research"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1093\/aje\/kwt062","article-title":"Long-term coffee consumption in relation to fracture risk and bone mineral density in women","volume":"178","author":"Hallstr\u00f6m H","year":"2013","journal-title":"Am J Epidemiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.jsbmb.2006.12.037","article-title":"Caffeine decreases vitamin D receptor protein expression and 1,25(OH)2D3 stimulated alkaline phosphatase activity in human osteoblast cells","volume":"103","author":"Rapuri PB","year":"2007","journal-title":"J Steroid Biochem Mol Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1006\/pmed.2000.0742","article-title":"Is caffeine associated with bone mineral density in young adult women?","volume":"31","author":"Conlisk AJ","year":"2000","journal-title":"Prev Med"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.pharmthera.2006.02.002","article-title":"Immunomodulatory effects of caffeine: Friend or foe?","volume":"111","author":"Horrigan LA","year":"2006","journal-title":"Pharmacol Therap"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.metabol.2003.08.004","article-title":"Studies on the mechanism of caffeine action in alveolar macrophages: Caffeine elevates cyclic adenosine monophosphate level and prostaglandin synthesis","volume":"53","author":"Jafari M","year":"2004","journal-title":"Metabolism"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0300-483X(00)00224-9","article-title":"Dose and time dependent effects of caffeine on superoxide release, cell survival and DNA fragmentation of alveolar macrophages from rat lung","volume":"149","author":"Jafari M","year":"2000","journal-title":"Toxicology"},{"key":"ref17","series-title":"Patology - Pathologic Basis of Disease","first-page":"91","volume-title":"Tissue renewal and repair: regeneration, healing, and fibrosis..","author":"Kumar V","year":"2005"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/S0278-6915(02)00094-7","article-title":"Effects of caffeine on bone and the calcium economy","volume":"40","author":"Heaney RP","year":"2002","journal-title":"Food Chem Toxicol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1002\/jor.21326","article-title":"Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing rats","volume":"29","author":"Liu SH","year":"2011","journal-title":"J Orthop Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"429","DOI":"10.3109\/09637486.2012.759184","article-title":"Caffeine regulates osteogenic differentiation and mineralization of primary adipose-derived stem cells and a bone marrow stromal cell line","volume":"64","author":"Su SJ","year":"2013","journal-title":"Int J Food Sci Nutr"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"698","DOI":"10.3390\/ijms9050698","article-title":"Caffeine induces cell death via activation of apoptotic signal and inactivation of survival signal in human osteoblasts","volume":"9","author":"Lu PZ","year":"2008","journal-title":"Int J Mol Sci"},{"key":"ref22","first-page":"421","article-title":"Caffeine content in Brazilian coffees","volume":"18","author":"Camargo MCR","year":"1998","journal-title":"Ci\u00eanc Tecnol Aliment"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0091-3057(97)00595-9","article-title":"Caffeine has similar pharmacokinetics and behavioral effects via the i.p. and p.o. routes of administration","volume":"60","author":"Wang Y","year":"1998","journal-title":"Pharmacol Biochem Behav"},{"key":"ref24","series-title":"Encyclopedia of Food Sciences and Nutrition (10 volumes)","volume-title":"Coffee","author":"Trugo L","year":"2003"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1902\/jop.2013.130179","article-title":"Coffee consumption and periodontal disease in males","volume":"85","author":"NG N","year":"2014","journal-title":"J Periodontol"}],"container-title":["Brazilian Dental Journal"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/bdj\/v26n2\/0103-6440-bdj-26-02-00175.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T08:28:41Z","timestamp":1566462521000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0103-64402015000200175&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["S0103-64402015000200175"],"URL":"https:\/\/doi.org\/10.1590\/0103-6440201300219","relation":{},"ISSN":["0103-6440"],"issn-type":[{"value":"0103-6440","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]}}}