{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T19:57:49Z","timestamp":1775246269223,"version":"3.50.1"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex\u00ae and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 X10 4 cells\/well in 96-well plates. Endodontic material extracts were prepared by placing sealer\/cement specimens (5X3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex\u00ae presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at \u00bd and \u00bc dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC.<\/jats:p>","DOI":"10.1590\/0103-6440201302115","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T16:58:18Z","timestamp":1377622698000},"page":"111-116","source":"Crossref","is-referenced-by-count":56,"title":["In Vitro Cytotoxicity of White MTA, MTA Fillapex\u00ae and Portland Cement on Human Periodontal Ligament Fibroblasts"],"prefix":"10.1590","volume":"24","author":[{"given":"Patr\u00edcia","family":"Yoshino","sequence":"first","affiliation":[{"name":"University of S\u00e3o Paulo (HRAC-USP), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celso Kenji","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo (HRAC-USP), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin Cristina da Silva","family":"Modena","sequence":"additional","affiliation":[{"name":"USP - University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Ferreira","family":"Santos","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo (HRAC-USP), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Renata","family":"Sipert","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo (HRAC-USP), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1097\/00004770-200005000-00010","article-title":"Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts","volume":"26","author":"Keiser K","year":"2000","journal-title":"J Endod"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/S0099-2399(06)80987-8","article-title":"Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report","volume":"21","author":"Torabinejad M","year":"1995","journal-title":"J Endod"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1046\/j.1365-2591.2002.00459.x","article-title":"Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells","volume":"35","author":"Huang FM","year":"2002","journal-title":"Int Endod J"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0099-2399(06)80967-2","article-title":"Physical and chemical properties of a new root-end filling material","volume":"21","author":"Torabinejad M","year":"1995","journal-title":"J Endod"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/S0099-2399(98)80074-5","article-title":"Cellular response to mineral trioxide aggregate","volume":"24","author":"Koh ET","year":"1998","journal-title":"J Endod"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1590\/S0103-64402011000400007","article-title":"Alfredo E, Leon JE, Perez DE. 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