{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:15:10Z","timestamp":1762506910096},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p>Abstract School-age children are frequently at high risk for the onset of biofilm-dependent conditions, including dental caries and periodontal diseases. The objective of this study was to evaluate the clinical efficacy of a dentifrice containing Eugenia uniflora Linn. (Surinam cherry) extract versus a triclosan-based comparator in treating gingivitis in children aged 10-12 years. The in vitro antibacterial potential of the dentifrice was tested against oral pathogens (Streptococcus mutans, Streptococcus oralis and Lactobacillus casei). Then a phase-II clinical trial was conducted with 50 subjects aged 10-12 years, with clinical signs of gingivitis. The subjects were randomly assigned to the experimental group (n=25) and control group (n=25), in which participants used the experimental dentifrice and a triclosan-based fluoridated dentifrice (Colgate Total 12(r)), respectively. Clinical examinations assessed the presence of gingivitis (primary outcome) and biofilm accumulation (secondary outcome) using the Gingival-Bleeding Index (GBI) and Simplified Oral Hygiene Index (OHI-S), respectively, at baseline and after seven days of tooth brushing 3x\/day. The data were analyzed using paired and unpaired t-test (GBI) and Wilcoxon and Mann-Whitney (OHI-S), with p\u22640.05. The experimental dentifrice showed efficient antibacterial activity in vitro. In the clinical trial, a significant reduction in gingival bleeding was observed in both experimental and control groups (p&lt;0.0001), with no statistical difference between them (p=0.178), although a small size effect was observed. Biofilm accumulation was only reduced in the control group (p=0.0039). In conclusion, E. uniflora dentifrice showed anti-gingivitis properties in children aged 10-12 years. Thus, it may be a potentially efficient and safe product to be used alternatively in preventive dental practice<\/jats:p>","DOI":"10.1590\/0103-6440201600769","type":"journal-article","created":{"date-parts":[[2016,9,17]],"date-time":"2016-09-17T17:48:54Z","timestamp":1474134534000},"page":"387-392","source":"Crossref","is-referenced-by-count":9,"title":["Eugenia uniflora Dentifrice for Treating Gingivitis in Children: Antibacterial Assay and Randomized Clinical Trial"],"prefix":"10.1590","volume":"27","author":[{"given":"Vanessa de Carvalho","family":"Jovito","sequence":"first","affiliation":[{"name":"Universidade Federal da Para\u00edba, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irlan Almeida","family":"Freires","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danilo Augusto de Holanda","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00e7al de Queiroz","family":"Paulo","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Dias de","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1601-5037.2012.00553.x","article-title":"Gingivitis and plaque scores of 8- to 11-year-old Burmese children following participation in a 2-year school-based toothbrushing programme","volume":"10","author":"Rosema NA","year":"2012","journal-title":"Int J Dent Hyg"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/idh.12011","article-title":"Oral hygiene and dental caries in 5- to 6-year-old children in Ajman, United Arab Emirates","volume":"11","author":"Hashim R","year":"2013","journal-title":"Int J Dent Hyg"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"4141","DOI":"10.1128\/JCM.42.9.4141-4146.2004","article-title":"Prevalence of periodontal pathogens in dental plaque of children","volume":"42","author":"Gafan GP","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1600-0757.2011.00425.x","article-title":"The global burden of periodontal disease: towards integration with chronic disease prevention and control","volume":"60","author":"Petersen PE","year":"2000","journal-title":"Periodontology"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"7329","DOI":"10.3390\/molecules20047329","article-title":"Antibacterial activity of essential oils and their isolated constituents against cariogenic bacteria: a systematic review","volume":"20","author":"Freires IA","year":"2015","journal-title":"Molecules"},{"key":"ref6","first-page":"147804","article-title":"Does scientific evidence for the use of natural products in the treatment of oral candidiasis exist? A systematic review","volume":"2015","author":"Ferreira GLS","year":"2015","journal-title":"Evid Based Complem Alternat Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"873907","DOI":"10.1155\/2013\/873907","article-title":"A randomized clinical trial of Schinus terebinthifolius mouthwash to treat biofilm-induced gingivitis","volume":"2013","author":"Freires IA","year":"2013","journal-title":"Evid Based Complem Alternat Med"},{"key":"ref8","first-page":"81","article-title":"In vivo evaluation of a dentifrice containing Eugenia uniflora L. extract on oral health indicators","volume":"9","author":"ovito VC","year":"2009","journal-title":"Pesq Bras Odontoped Clin Integr"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2981","DOI":"10.1039\/C4FO00509K","article-title":"Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells","volume":"5","author":"Soares JD","year":"2014","journal-title":"Food Funct"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1016\/j.fct.2012.05.002","article-title":"Essential oil of the leaves of Eugenia uniflora L.: Antioxidant and antimicrobial properties","volume":"50","author":"Victoria FN","year":"2012","journal-title":"Food Chem Toxicol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0378-8741(87)90128-0","article-title":"Preliminary pharmacological studies on Eugenia uniflora leaves: xanthine oxidase inhibitory activity","volume":"21","author":"Schmeda-Hirschmann G","year":"1987","journal-title":"J. Ethnopharmacol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disk method","volume":"45","author":"Bauer AW","year":"1969","journal-title":"Am J Clinical Pathol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"143","DOI":"10.2307\/2530297","article-title":"One-sample multiple testing procedure for Phase II clinical trials","volume":"38","author":"Fleming TR","year":"1982","journal-title":"Biometrics"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis JR","year":"1977","journal-title":"Biometrics"},{"key":"ref15","first-page":"229","article-title":"Problems and proposals for recording gingivitis and plaque","volume":"25","author":"Ainamo J","year":"1975","journal-title":"Int. Dent J"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"7","DOI":"10.14219\/jada.archive.1964.0034","article-title":"The simplified oral hygiene index","volume":"68","author":"Greene JC","year":"1964","journal-title":"J Amer Dent Ass"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1590\/0103-6440201300027","article-title":"Antimicrobial activity of toothpastes containing natural extracts, chlorhexidine or triclosan","volume":"25","author":"Rossi AD","year":"2014","journal-title":"Braz Dent J"},{"key":"ref18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047722","article-title":"Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis","volume":"7","author":"Gross EL","year":"2012","journal-title":"PLoS ONE"},{"key":"ref19","first-page":"77","article-title":"Lactobacilli in human dental caries and saliva","volume":"105","author":"Smith SI","year":"2001","journal-title":"Microbios"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.cden.2010.03.002","article-title":"Microbiology of dental plaque biofilms and their role in oral health and caries","volume":"54","author":"Marsh PD","year":"2010","journal-title":"Dent. 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