{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:32:15Z","timestamp":1787761935288,"version":"build-2784847793"},"reference-count":12,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>Abstract Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in the periodontal disease pathogenesis remains unclear. Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival crevicular fluid from individuals having periodontitis aggravated by type 2 diabetes. Twelve patients (56.2 \u00b17.2 yrs) with severe form of chronic periodontitis aggravated by type 2 diabetes were included. Clinical and metabolic data were gathered. Gingival crevicular fluid was collected using filter strips from deep and shallow sites. MPs were detected by flow cytometry according to their size (&lt; 1 \u00b5m) and the expression of surface markers (CD66b for neutrophil-derived MPs and CD41b for platelet-derived MPs). All samples were positive for the antibodies. Median levels of CD66b+ MPs and CD41b+ MPs were, respectively, 3,677.0 (2,553.2 - 9,059.8) MP\/\u00b5L and 520.7 (432.9 - 766.1) MP\/\u00b5L in deep sites. In shallow sites, the corresponding values were 2,644.9 (1,451.5 - 3,858.9) MP\/\u00b5L and 371.2 (287.2 - 692.7) MP\/\u00b5L. There was no significant difference between deep and shallow sites (p&gt;0.05). In conclusion, this study reported the presence of neutrophil and platelet derived microparticles in gingival crevicular fluid from individuals having severe periodontitis and type 2 diabetes.<\/jats:p>","DOI":"10.1590\/0103-6440201701560","type":"journal-article","created":{"date-parts":[[2017,12,2]],"date-time":"2017-12-02T17:30:28Z","timestamp":1512235828000},"page":"675-678","source":"Crossref","is-referenced-by-count":4,"title":["Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Type 2 Diabetes"],"prefix":"10.1590","volume":"28","author":[{"given":"Carlos Marcelo","family":"Figueredo","sequence":"first","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo","family":"Lira Junior","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela Rubim","family":"Sete","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Guimar\u00e3es","family":"Fischer","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/nrrheum.2014.19","article-title":"Emerging role of extracellular vesicles in inflammatory diseases","volume":"10","author":"Buzas EI","year":"2014","journal-title":"Nat Rev Rheumatol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1002\/art.10312","article-title":"Elevated levels of platelet microparticles are associated with disease activity in rheumatoid arthritis","volume":"46","author":"Knijff-Dutmer EA","year":"2002","journal-title":"Arthritis 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