{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:33:30Z","timestamp":1761060810085,"version":"3.37.3"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder\u00ae, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover\u00ae, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover\u00ae coating before sterilization; DPE2, BisCover\u00ae coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells\/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE\/DEAD\u00ae BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL\u00ae v.2.0 software and total biovolume (\u00b5m3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p&lt;0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder\u00ae coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures.<\/jats:p>","DOI":"10.1590\/0103-6440201901953","type":"journal-article","created":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T19:47:20Z","timestamp":1559418440000},"page":"266-271","source":"Crossref","is-referenced-by-count":8,"title":["Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6383-1050","authenticated-orcid":false,"given":"Flora Freitas Fernandes","family":"T\u00e1vora","sequence":"first","affiliation":[{"name":"USC - Universidade do Sagrado Cora\u00e7\u00e3o, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1258-9265","authenticated-orcid":false,"given":"Ana Paula Chappuis","family":"Chocano","sequence":"additional","affiliation":[{"name":"USP - Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1215-5299","authenticated-orcid":false,"given":"Denise Gusm\u00e3o de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"UniNorte - Laureate International Universities, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5663-3710","authenticated-orcid":false,"given":"Jefferson Ricardo","family":"Pereira","sequence":"additional","affiliation":[{"name":"UNISUL - Universidade do Sul de Santa Catarina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1427-936X","authenticated-orcid":false,"given":"Ricardo Sergio","family":"Almeida","sequence":"additional","affiliation":[{"name":"UEL - Universidade Estadual de Londrina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7086-2667","authenticated-orcid":false,"given":"Karin Hermana","family":"Neppelenbroek","sequence":"additional","affiliation":[{"name":"USP - Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6609-9934","authenticated-orcid":false,"given":"Vin\u00edcius Carvalho","family":"Porto","sequence":"additional","affiliation":[{"name":"USP - Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2019,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1532-849X.2011.00698.x","article-title":"Epidemiology and etiology of denture stomatitis","volume":"20","author":"Gendreau L","year":"2011","journal-title":"J Prosthodont"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.tripleo.2003.04.002","article-title":"Denture stomatitis: a role for Candida biofilms","volume":"98","author":"Ramage G","year":"2004","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endo"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1590\/0103-6440201302382","article-title":"In vitro antimicrobial activity of an experimental dentifrice based on Ricinus communis","volume":"25","author":"Leite VM","year":"2014","journal-title":"Braz Dent J"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.ijid.2011.06.006","article-title":"Epidemiology, clinical characteristics, and outcome of candidemia: experience in a tertiary referral center in the UK","volume":"15","author":"Das I","year":"2011","journal-title":"Int J Infect Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1601-5037.2009.00413.x","article-title":"The denture hygiene, denture stomatitis and role of dental hygienist","volume":"8","author":"Sadig W","year":"2010","journal-title":"Int J Dent Hyg"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/nmat856","article-title":"Self-cleaning surfaces - virtual realities","volume":"2","author":"Blossey R","year":"2003","journal-title":"Nature materials"},{"key":"ref7","first-page":"308","article-title":"In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria","volume":"20","author":"Pereira-Cenci T","year":"2007","journal-title":"Int J Prosthodont"},{"key":"ref8","first-page":"157","article-title":"A comparison of three hard chairside denture reline materials","volume":"11","author":"Haywood J","year":"2003","journal-title":"Part I. 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