{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:54:20Z","timestamp":1740142460521,"version":"3.37.3"},"reference-count":33,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract The aim of this study was to evaluate the effect of acute sepsis in the periodontal ligament, alveolar and furcation bone in absence of periodontitis induction through histological and immunohistochemical analyses. A septic rat model was established by cecal ligation and puncture (CLP). Twelve rats were randomly divided into CLP (n=6) and Sham (n=6) groups. The animals were euthanized at 24 h and hemimandibles were submitted to histomorfometric (bone matrix, collagenous fibers, fibroblasts, osteocytes, inflammatory cells, and blood vessels) and immunohistochemical (BMP-2\/4, RANKL and osteocalcin) evaluation in alveolar bone, furcation bone and periodontal ligament. Our results demonstrated that histomorphometric parameters were similar in alveolar bone, furcation bone and periodontal ligament of Sham and CLP rats. Regarding to immunohistochemical analyses, the number of BMP-2\/4 and RANKL immunolabeled cells was also similar in both groups. Furthermore, it was detected a reduction in the osteocalcin immunolabeled cells in periodontal ligaments of CLP compared to Sham rats (p=0.0014). In conclusion, the acute sepsis induction resulted in reduced number of osteocalcin labelled cells in periodontal ligament region. Moreover, no significant histological differences were observed in the periodontium of rats under acute sepsis. Considering the role of osteocalcin in bone remodeling, the study contributes to revealing the importance of careful periodontal evaluation in the presence of sepsis.<\/jats:p>","DOI":"10.1590\/0103-6440202003024","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T14:59:34Z","timestamp":1591973974000},"page":"143-151","source":"Crossref","is-referenced-by-count":1,"title":["Experimental Acute Sepsis Reduced Number of Osteocalcin Immunolabeled Cells in Periodontal Ligament"],"prefix":"10.1590","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2077-8373","authenticated-orcid":false,"given":"Gabriela Veloso Vieira da Silva","family":"Pinheiro","sequence":"first","affiliation":[{"name":"Universidade Estadual de Ponta Grossa,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2104-5780","authenticated-orcid":false,"given":"Robinson","family":"Sabino-Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de Uberl\u00e2ndia,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2180-8223","authenticated-orcid":false,"given":"Melissa Rodrigues de","family":"Araujo","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7219-368X","authenticated-orcid":false,"given":"Shaiene Patr\u00edcia","family":"Gomes","sequence":"additional","affiliation":[{"name":"Universidade Positivo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2769-1094","authenticated-orcid":false,"given":"Stephanie Wutke","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Uberl\u00e2ndia,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9125-7503","authenticated-orcid":false,"given":"Em\u00edlia Maria Gomes","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Universidade Federal de Uberl\u00e2ndia,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5748-8244","authenticated-orcid":false,"given":"L\u00e9ia","family":"Cardoso-Sousa","sequence":"additional","affiliation":[{"name":"Universidade Federal de Uberl\u00e2ndia,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6374-1605","authenticated-orcid":false,"given":"Carla Castiglia","family":"Gonzaga","sequence":"additional","affiliation":[{"name":"Universidade Positivo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1553-5852","authenticated-orcid":false,"given":"Marcela","family":"Claudino","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Ponta Grossa,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2020,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1001\/jama.2016.0289","article-title":"Developing a new definition and assessing new clinical criteria for septic shock: for the third international consensus definitions for sepsis and septic shock (sepsis-3)","volume":"315","author":"Shankar-Hari M","year":"2016","journal-title":"JAMA"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1164\/rccm.201504-0781OC","article-title":"Assessment of global incidence and mortality of hospital-treated sepsis. Current estimates and limitations","volume":"193","author":"Fleischmann C","year":"2016","journal-title":"Am J Respir Crit Care Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1056\/NEJMp1707170","article-title":"Recognizing sepsis as a global health priority - a who resolution","volume":"377","author":"Reinhart K","year":"2017","journal-title":"N Engl J Med"},{"issue":"7","key":"ref4","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3389\/fcell.2019.00108","article-title":"Sepsis: Inflammation is a necessary evil","volume":"20","author":"Nedeva C","year":"2019","journal-title":"Front Cell Dev Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s00281-017-0639-8","article-title":"Cytokine storm and sepsis disease pathogenesis","volume":"39","author":"Chousterman BG","year":"2017","journal-title":"Semin Immunopathol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1600-0463.2010.02705.x","article-title":"Multiplex cytokine profiling in patients with sepsis","volume":"119","author":"Mera S","year":"2011","journal-title":"APMIS"},{"key":"ref7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0222575","article-title":"Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis","volume":"14","author":"Cardoso-Sousa L","year":"2019","journal-title":"PLoS One"},{"key":"ref8","first-page":"23:290","article-title":"Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis","volume":"29","author":"Tu H","year":"2019","journal-title":"Crit Care"},{"key":"ref9","first-page":"356841","article-title":"Spontaneous periodontitis development in diabetic rats involves an unrestricted expression of inflammatory cytokines and tissue destructive factors in the absence of major changes in commensal oral microbiota","volume":"12","author":"Claudino M","year":"2012","journal-title":"Exp Diabetes Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1371\/journal.pone.0001320","article-title":"Alloxan-induced diabetes triggers the development of periodontal disease in rats","volume":"2","author":"Claudino M","year":"2007","journal-title":"Plos One"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40608-016-0082-8","article-title":"High-fat diet-induced obesity triggers alveolar bone loss and spontaneous periodontal disease in growing mice","volume":"3","author":"Fujita Y","year":"2016","journal-title":"BMC Obes"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1016\/j.archoralbio.2013.03.006","article-title":"Obesity may increase the occurrence of spontaneous periodontal disease in Wistar rats","volume":"58","author":"Cavagni J","year":"2013","journal-title":"Arch Oral Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1016\/j.immuni.2016.05.012","article-title":"Sepsis-induced osteoblast ablation causes immunodeficiency","volume":"44","author":"Terashima A","year":"2016","journal-title":"Immunity"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"302","DOI":"10.3109\/08941939.2015.1111474","article-title":"Histopathological and biochemical effects of ecballium elaterium on sepsis-induced lung injury","volume":"29","author":"Demir M","year":"2016","journal-title":"J Invest Surg"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s12931-015-0266-7","article-title":"Role of pulmonary microvascular endothelial cell apoptosis in murine sepsis-induced lung injury in vivo","volume":"16","author":"Gill SE","year":"2015","journal-title":"Respir Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1080\/08941939.2016.1181230","article-title":"Effects of ecballium elaterium on proinflammatory cytokines in a rat model of sepsis","volume":"29","author":"Arslan MS","year":"2016","journal-title":"J Invest Surg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.archoralbio.2015.08.015","article-title":"Diabetes triggers the loss of tooth structure associated to radiographical and histological dental changes and its evolution to progressive pulp and periapical lesions in rats","volume":"60","author":"Claudino M","year":"2015","journal-title":"Arch Oral Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.3892\/mmr.2015.3891","article-title":"Effects of estrogen deficiency on microstructural changes in rat alveolar bone proper and periodontal ligament","volume":"12","author":"Liu Z","year":"2015","journal-title":"Mol Med Rep"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"484280","DOI":"10.1155\/2014\/484280","article-title":"Bone remodelling markers in rheumatoid arthritis","volume":"2014","author":"Fardellone P","year":"2014","journal-title":"Mediators Inflamm"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1097\/00003246-200204000-00020","article-title":"Increased bone resorption in the critically ill: Association with sepsis and increased nitric oxide production","volume":"30","author":"Smith LM","year":"2002","journal-title":"Crit Care Med"},{"key":"ref21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0171689","article-title":"Transcriptomic meta-analysis reveals up-regulation of gene expression functional in osteoclast differentiation in human septic shock.","volume":"12","author":"Mukhopadhyay S","year":"2017","journal-title":"Plos One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/nrendo.2016.12","article-title":"BMP signalling in skeletal development, disease and repair","volume":"12","author":"Salazar VS","year":"2016","journal-title":"Nat Rev Endocrinol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/nyas.13470","article-title":"Osteocalcin and osteopontin influence bone morphology and mechanical properties","volume":"1409","author":"Bailey S","year":"2017","journal-title":"Ann N Y Acad Sci"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"611","DOI":"10.2478\/s11658-014-0216-2","article-title":"Gene expression patterns in bone following lipopolysaccharide stimulation","volume":"19","author":"Yang J","year":"2014","journal-title":"Cell Mol Biol Lett"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1046\/j.1469-7580.2003.00158.x","article-title":"Expression of bone morphogenetic proteins and cartilage-derived morphogenetic proteins during osteophyte formation in humans","volume":"202","author":"Zoricic S","year":"2003","journal-title":"J Anat"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.archoralbio.2011.05.009","article-title":"Comparative immunohistochemical expression of RANK, RANKL and OPG in radicular and dentigerous cysts","volume":"56","author":"Moraes M","year":"2011","journal-title":"Arch Oral Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1177\/0022034510376402","article-title":"Destructive and protective roles of cytokines in periodontitis: a re-appraisal from host defense and tissue destruction viewpoints","volume":"89","author":"Garlet GP","year":"2010","journal-title":"J Dent Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0162109","article-title":"mice survival and plasmatic cytokine secretion in a \u201ctwo hit\u201d model of sepsis depend on intratracheal pseudomonas aeruginosa bacterial load","volume":"11","author":"Restagno D","year":"2016","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"611","DOI":"10.2478\/s11658-014-0216-2","article-title":"Gene expression patterns in bone following Lipopolysaccharide stimulation","volume":"19","author":"Yang J","year":"2014","journal-title":"Cell Mol Biol Lett"},{"issue":"10","key":"ref30","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.3389\/fimmu.2019.01982","article-title":"IL-17, IL-27, and IL-33: A novel axis linked to immunological dysfunction during sepsis","volume":"22","author":"Morrow KN","year":"2019","journal-title":"front immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1159\/000479836","article-title":"IL-33 Attenuates sepsis by inhibiting IL-17 receptor signaling through upregulation of SOCS3","volume":"42","author":"Lv R","year":"2017","journal-title":"Cell Physiol Biochem"},{"issue":"13","key":"ref32","article-title":"IL-7 treatment augments and prolongs sepsis-induced expansion of IL-10-producing B lymphocytes and myeloid-derived suppressor cells","volume":"21","author":"Kulkarni U","year":"2018","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1097\/CCM.0000000000002732","article-title":"Sepsis reduces bone strength before morphologic changes are identifiable","volume":"45","author":"Puthucheary ZA","year":"2017","journal-title":"Crit Care Med"}],"container-title":["Brazilian Dental Journal"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/bdj\/v31n2\/1806-4760-bdj-31-02-143.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T21:21:02Z","timestamp":1611436862000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0103-64402020000200143&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["S0103-64402020000200143"],"URL":"https:\/\/doi.org\/10.1590\/0103-6440202003024","relation":{},"ISSN":["1806-4760","0103-6440"],"issn-type":[{"type":"electronic","value":"1806-4760"},{"type":"print","value":"0103-6440"}],"subject":[],"published":{"date-parts":[[2020,4]]}}}