{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:19:32Z","timestamp":1774437572315,"version":"3.50.1"},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract This study evaluated the cytotoxicity of Sealapex Xpress and Real Seal XT and their effect on macrophage activation. J774.1 macrophages were incubated with Sealapex Xpress and Seal Real XT (0.1, 1.0, and 10 mg\/mL) for 24 and 48 h. Cell viability was assessed by the MTT assay and macrophage activation was measured by pro- and anti-inflammatory cytokine production using ELISA. Data were analyzed using one-way ANOVA and Tukey\u2019s post-test (a=0.05). Cell viability was not affected with 0.1 or 1.0 mg\/mL of extracts of Sealapex Xpress and Real Seal XT at 24 and 48 h (p&gt;0.05), but was significantly lower when cells were exposed to 10 mg\/mL of both sealers (p&lt;0.05). Sealapex Xpress inhibited the production of TNF-a, whereas Real Seal XT induced TNF-a secretion at 24 h (p&lt;0.05). IL-6 production was induced by Real Seal XT, but not by Sealapex Xpress (p&lt;0.05). Real Seal XT and Sealapex Xpress induced the secretion of anti-inflammatory IL-10. IL-4 was not detected in any group. In conclusion, both sealers had low toxicity but differentially activated macrophages. Macrophage activation by Sealapex Xpress was characterized by inhibition of TNF-a and induction of IL-10, whereas Real Seal XT induced IL-6 solely.<\/jats:p>","DOI":"10.1590\/0103-6440202103330","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T12:02:15Z","timestamp":1617364935000},"page":"48-52","source":"Crossref","is-referenced-by-count":4,"title":["Cytotoxicity and Inflammatory Mediators Release by Macrophages Exposed to Real Seal XT and Sealapex Xpress"],"prefix":"10.1590","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7118-6859","authenticated-orcid":false,"given":"L\u00e9a Assed Bezerra da","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade de S\u00e3o Paul,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8615-4876","authenticated-orcid":false,"given":"L\u00eddia Regina da Costa","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paul,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7696-7600","authenticated-orcid":false,"given":"Manoel Dami\u00e3o","family":"de Sousa-Neto","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paul,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1044-0937","authenticated-orcid":false,"given":"Maya Fernanda Manfrin","family":"Arnez","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paul,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1772-5425","authenticated-orcid":false,"given":"Frederic","family":"Barnett","sequence":"additional","affiliation":[{"name":"Albert Einstein Medical Center,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9785-5766","authenticated-orcid":false,"given":"Patricia Mar\u00eda Gat\u00f3n","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Universitat de Barcelona,  Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4999-8305","authenticated-orcid":false,"given":"L\u00facia Helena","family":"Faccioli","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paul,  Brazil; Universidade de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-532X","authenticated-orcid":false,"given":"Francisco Wanderley Garcia","family":"Paula-Silva","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paul,  Brazil; Universidade de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2021,2]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1590\/1678-775720130174","article-title":"A microleakage study of gutta-percha\/AH Plus and Resilon\/Real self-etch systems after different irrigation protocols","volume":"22","author":"Prado M","year":"2014","journal-title":"J Appl Oral Sci"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1016\/j.joen.2014.01.040","article-title":"Sealapex Xpress and RealSeal XT feature tissue compatibility in vivo","volume":"40","author":"Silva LA","year":"2014","journal-title":"J Endod"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/j.joen.2008.03.029","article-title":"A comparison study of periapical repair in dogs\u2019 teeth using Roeko Seal and AH plus root canal sealers: the histopathological evaluation","volume":"34","author":"Leonardo MR","year":"2008","journal-title":"J Endod"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/S0099-2399(97)80296-8","article-title":"Calcium hydroxide root canal sealers - 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