{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:29:26Z","timestamp":1789468166759,"version":"build-2803163510"},"reference-count":31,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract The endodontic revascularization may be an alternative treatment for necrotic immature teeth, however, several treatment steps may cause tooth discoloration. This study evaluated the use of three calcium silicate-based cements with different radiopacifying agents on the color alteration (\u2206E) of extracted premolars after simulation of revascularization. Forty single rooted extracted premolars were shaped with #1-6 gates Glidden drills, rinsed with sodium hypochlorite, and filled with fresh human blood. Three calcium silicate-based cements with different radiopacifying agents (bismuth oxide - CSBi, calcium tungstate - CSW, and zirconium oxide - CSZr) were applied over the blood clot (n=10). The control group received the application of a temporary zinc oxide-based cement (TFZn) (n=10). \u2206E was measured with a spectrophotometer, using the L*a*b* color system of the International Commission on Illumination (CIELab), in different times: prior to the preparation of the access cavity (t0); right after treatment (t1); and after one (t2), two (t3), three (t4) and four (t5) months. The tooth site for color evaluation was standardized by silicon matrix, the color reading was performed 3 times per tooth, and the teeth were stored in 37\u00ba water between evaluations. \u2206E, whiteness (WID index) and yellowness (b*) were evaluated. Data were subjected to one-way ANOVA and repeated measures ANOVA, followed by Tukey\u2019s post hoc test (\u03b1=0.05). All groups were similar in \u2206E1 (t0-t1). The \u2206E was the lowest and constant in the control group. In all evaluation times, CSBi presented the highest \u2206E (p&lt;0.01). CSW and CSZr were similar in all evaluated times and presented intermediate \u2206E values. WID index from CSBi and CSW presented more distancing from \u2018white\u2019 reference. CSBi presented the greatest decrease in yellowness (b* value). The cement containing bismuth oxide presented the highest color alteration values. All tested calcium silicate-based cements presented clinically perceptible discoloration. Calcium tungstate and zirconium oxide may be used as alternative radiopacifiers to decrease tooth discoloration after endodontic tooth revascularization.<\/jats:p>","DOI":"10.1590\/0103-6440202103700","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T12:02:49Z","timestamp":1617364969000},"page":"53-58","source":"Crossref","is-referenced-by-count":9,"title":["Tooth Discoloration Using Calcium Silicate-Based Cements For Simulated Revascularization in Vitro"],"prefix":"10.1590","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4817-3106","authenticated-orcid":false,"given":"Rui Barbosa","family":"Marques Junior","sequence":"first","affiliation":[{"name":"University of Taubat\u00e9,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1009-0869","authenticated-orcid":false,"given":"Kusai","family":"Baroudi","sequence":"additional","affiliation":[{"name":"University of Taubat\u00e9,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9022-4424","authenticated-orcid":false,"given":"Alison Fl\u00e1vio Campos dos","family":"Santos","sequence":"additional","affiliation":[{"name":"University of Taubat\u00e9,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8846-1757","authenticated-orcid":false,"given":"Denise","family":"Pontes","sequence":"additional","affiliation":[{"name":"University of Taubat\u00e9,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4301-2760","authenticated-orcid":false,"given":"Marina","family":"Amaral","sequence":"additional","affiliation":[{"name":"University of Taubat\u00e9,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2021,2]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"13941","DOI":"10.1038\/s41598-017-14412-x","article-title":"Treatment of necrotic teeth by apical revascularization: meta-analysis","volume":"7","author":"He L","year":"2017","journal-title":"Sci Rep"},{"key":"ref2","first-page":"ZC34","article-title":"Comparison of the effect of PRP, PRF and induced bleeding in the revascularization of teeth with necrotic pulp and open apex: a triple blind randomized clinical trial","volume":"11","author":"Shivashankar VY","year":"2017","journal-title":"J Clin Diagn Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1111\/ipd.12474","article-title":"Clinical and radiographic evaluation of biodentine and mineral trioxide aggregate in revascularization of non-vital immature permanent anterior teeth (randomized clinical 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