{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T17:12:34Z","timestamp":1794157954971,"version":"4.8.0"},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract This study aimed to evaluate the microstructure formed after the chemical treatment of teeth, for the development of autogenous grafts from the demineralized dentin matrix (DDM) technique, in order to identify the most efficient demineralizing solution. The specimens, originating from the root and coronal portion, were submitted to ultrasonic cleaning and drying in an oven for 1h at 100 \u00baC. Then, the density was determined by Archimedes\u2019 principle for each specimen, using distilled water as immersion liquid. The samples were separated into five groups: Control group: negative control, Distilled water;EDTA group: positive control, trisodium EDTA; NaOCl group: 2.5% sodium hypochlorite; HCl-0.6M group: 0.6M hydrochloric acid; and H2O2\/H2SO4 group: hydrogen peroxide and sulfuric acid. Each specimen was immersed for 1h in the corresponding group descaling solution at 60 \u00baC. Subsequently, the mass loss and density of the treated specimens were determined by Archimedes\u2019 principle. Ultimately, the specimens of each group were characterized by microtomography, Scanning Electron Microscopy, and Energy Dispersive Spectrometry X-ray (SEM-EDS). The results demonstrated that the H2O2\/H2SO4 solution allowed the formation of interconnected micropores, suggesting better pore structures for application in scaffolds, when compared to the other studied solutions.<\/jats:p>","DOI":"10.1590\/0103-6440202305353","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T11:16:05Z","timestamp":1698059765000},"page":"72-84","source":"Crossref","is-referenced-by-count":4,"title":["Demineralized dentin matrix technique - a comparison of different demineralizing solutions"],"prefix":"10.1590","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2687-0165","authenticated-orcid":false,"given":"Fabiano Luiz","family":"Heggendorn","sequence":"first","affiliation":[{"name":"UNIGRANRIO,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7115-5315","authenticated-orcid":false,"given":"M\u00e1rcio Batista do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"UNIGRANRIO,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5086-6051","authenticated-orcid":false,"given":"Andreza Menezes","family":"Lima","sequence":"additional","affiliation":[{"name":"National Institute of Technology,  USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5299-4241","authenticated-orcid":false,"given":"Alexandre Antunes","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"National Institute of Technology,  USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2023,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/jbm.a.33236","article-title":"Acid-insoluble human dentin as carrier material for recombinant human BMP-2","volume":"100","author":"Murata M","year":"2012","journal-title":"J Biomed Mater Res A"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.5125\/jkaoms.2012.38.1.2","article-title":"Clinical application of auto-tooth bone graft material","volume":"38","author":"Park S","year":"2012","journal-title":"J Koran Assoc Oral Maxillofac Surg"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.injury.2011.06.014","article-title":"What should be the characteristics of the ideal graft replacement cap? combining scaffolds with growth factors and\/or stem cells","volume":"42","author":"Janicki P","year":"2011","journal-title":"Injury"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1002\/jbm.a.35790","article-title":"Different methods of dentin processing for application in bone tissue engineering: a systematic review","volume":"104","author":"Tabatabaei FS","year":"2016","journal-title":"J Biomed Mater Res A"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0300-9785(86)80136-3","article-title":"Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dogs","volume":"15","author":"Catanzaro-Guimar\u00e3es SA","year":"1986","journal-title":"Int J Oral Maxillofac Surg"},{"issue":"23","key":"ref6","first-page":"493","article-title":"Ridge augmentation using sticky bone: a combination of human tooth allograft and autologous fibrin glue","author":"Joshi CP","year":"2019","journal-title":"J Indian Soc Periodontol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.jconrel.2021.04.003","article-title":"Nanotechnology, and scaffold implantation for the effective repair of injured organs: an overview on hard tissue engineering","volume":"333","author":"Abdollahiyan P","year":"2021","journal-title":"J Control Release"},{"key":"ref8","series-title":"Method for producing a bone transplant material, and bone transplant material produced by the same","author":"Park CS","year":"2013"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"120","DOI":"10.4103\/jips.jips_62_17","article-title":"Demineralized dentin matrix scaffolds for alveolar bone engineering","volume":"17","author":"Um IW","year":"2017","journal-title":"J Indian Prosthodont Soc"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/j.jmrt.2021.08.083","article-title":"Decellularized and biological scaffolds in dental and craniofacial tissue engineering: a comprehensive overview","volume":"15","author":"Yazdanian M","year":"2021","journal-title":"J Mater Res Technol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21608\/jfcr.2021.181031","article-title":"Effect of sodium hypochlorite (NaOCl) for pretreatment of early demineralized enamel lesions in enhancing the remineralization capacity of self-assembling peptide (in-vitro study)","volume":"1","author":"Elwazir A","year":"2021","journal-title":"J Fundam Clin Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.tripleo.2009.10.017","article-title":"Development of a novel bone grafting material using autogenous teeth","volume":"109","author":"Kim YK","year":"2010","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1097\/ID.0000000000000011","article-title":"Bone Grafts using autogenous tooth blocks: A case series","volume":"22","author":"Kim YK","year":"2013","journal-title":"Implant Dent"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/0003-9969(67)90095-7","article-title":"Bone induction by decalcified dentine implanted into oral, osseous and muscle tissues","volume":"12","author":"Yeomans JD","year":"1967","journal-title":"Arch Oral Biol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1177\/0885328219866039","article-title":"Chemical and thermal deproteinization of human demineralized tooth matrix: Physicochemical characterization and osteoblast cell biocompatibility","volume":"34","author":"Tanwatana S","year":"2019","journal-title":"J Biomater Appl"},{"key":"ref16","series-title":"ASTM B962-17. Standard Test Methods for Density of Compacted or Sintered Powder Metallurgy (PM) Products Using Archimedes' Principle","year":"2017"},{"key":"ref17","series-title":"CRC Handbook of Chemistry and Physics","author":"Lide DR","year":"2009","edition":"89th ed."},{"key":"ref18","series-title":"Phillips\u2019 Science of Dental Materials","author":"Anusavice KJ","year":"2013","edition":"12th ed."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1097\/00004770-200109000-00006","article-title":"Effects of chelating agents and sodium hypochlorite on mineral content of root dentin","volume":"27","author":"Dogan H","year":"2001","journal-title":"J Endod"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1097\/01.don.0000137643.54109.81","article-title":"Effects of endodontic irrigation solutions on mineral content of root canal dentin using ICP-AES technique","volume":"31","author":"Ari H","year":"2005","journal-title":"J Endod"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.joen.2007.12.016","article-title":"Evaluation of root canal dentin after nd:yag laser irradiation and treatment with five different irrigation solutions: a preliminary study","volume":"34","author":"Gurbuz T","year":"2008","journal-title":"J Endod"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.biomaterials.2015.02.110","article-title":"Biomaterials based skeletal muscle tissue engineering strategies: existing technologies and future trends","volume":"53","author":"Qazi TH","year":"2015","journal-title":"Biomaterials"},{"key":"ref23","series-title":"Graded cellular bone scaffolds. Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications","first-page":"75","author":"Limmahakhun S","year":"2017","edition":"Baino F"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1016\/j.biomaterials.2005.08.035","article-title":"A comparison of micro CT with other techniques used in the characterization of scaffolds","volume":"27","author":"Ho ST","year":"2006","journal-title":"Biomaterials"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.biomaterials.2009.09.063","article-title":"The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering","volume":"31","author":"Murphy CM","year":"2010","journal-title":"Biomaterials"},{"key":"ref26","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2021.112437","article-title":"Development of hierarchical porous bioceramic scaffolds with controlled micro\/nano surface topography for accelerating bone regeneration","volume":"130","author":"Zhang H","year":"2021","journal-title":"Mater Sci Eng C"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"377","DOI":"10.4161\/cam.4.3.11747","article-title":"Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds","volume":"4","author":"Murphy CM","year":"2010","journal-title":"Cell Adh Migr"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3914","DOI":"10.1557\/s43578-021-00403-2","article-title":"Shaping and properties of thermoplastic scaffolds in tissue regeneration: the effect of thermal history on polymer crystallization, surface characteristics and cell fate","volume":"36","author":"Calore AR","year":"2021","journal-title":"J Mater Res"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"512","DOI":"10.4103\/JCD.JCD_102_19","article-title":"Scanning electron microscopy evaluation of dentin ultrastructure after surface demineralization","volume":"23","author":"Kuntze MM","year":"2020","journal-title":"J Conserv Dent"}],"container-title":["Brazilian Dental Journal"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0103-64402023000400072&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T11:16:18Z","timestamp":1698059778000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0103-64402023000400072&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["S0103-64402023000400072"],"URL":"https:\/\/doi.org\/10.1590\/0103-6440202305353","relation":{},"ISSN":["1806-4760","0103-6440"],"issn-type":[{"value":"1806-4760","type":"electronic"},{"value":"0103-6440","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8]]}}}