{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:07:50Z","timestamp":1753888070888,"version":"3.41.2"},"reference-count":31,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract The aim of this study was to evaluate the effects of long-term alendronate administration on bone repair and mineralization around osseointegrated implants in rats. A total of 160 female Wistar rats were randomly assigned to two groups: the control group (CTL) and the alendronate group (ALD). The ALD group received a subcutaneous injection of sodium alendronate (1 mg\/kg\/week), while the CTL group received weekly injections of saline solution. After 120 days of treatment, a bilateral implant was placed in the tibia of each rat. Ten rats from each group were euthanized at 5, 10, 15, 20, 25, 30, 45, or 60 days post-surgery. Picro-sirius red staining was utilized to assess the distribution and arrangement of collagen fibers near the implant threads. Bone mineralization mapping of the native bone adjacent to the implant was performed using images obtained through scanning electron microscopy (SEM) across all follow-up periods. SEM-based mineralization mapping revealed an increase in both the degree and homogeneity of bone mineralization in the ALD group compared to the CTL group. Alendronate administration affected collagen arrangement and distribution, leading to a connective tissue with reduced organization and thinner collagen fiber bundles. In conclusion, the findings demonstrated that alendronate administration resulted in a higher degree and homogeneity of bone mineralization, accompanied by reduced collagen content and organization, suggesting an impairment in bone remodeling around dental implants.<\/jats:p>","DOI":"10.1590\/0103-644020235751","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T12:32:56Z","timestamp":1733142776000},"source":"Crossref","is-referenced-by-count":0,"title":["Effect of Long-Term Treatment with Alendronate on Bone Repair and Mineralization Around Implants in Rats"],"prefix":"10.1590","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8522-9466","authenticated-orcid":false,"given":"Mario Henrique Arruda","family":"Verzola","sequence":"first","affiliation":[{"name":"Sao Paulo State University,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0027-6686","authenticated-orcid":false,"given":"Fausto","family":"Frizzera","sequence":"additional","affiliation":[{"name":"Federal University of Espirito Santo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5756-5828","authenticated-orcid":false,"given":"Paulo Sergio","family":"Cerri","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-8540","authenticated-orcid":false,"given":"Ubirajara","family":"Pereira Rodrigues-Filho","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2685-8526","authenticated-orcid":false,"given":"Silvana Regina Perez","family":"Orrico","sequence":"additional","affiliation":[{"name":"Sao Paulo State University,  Brazil; Union of the Colleges of the Great Lakes,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1110-6233","authenticated-orcid":false,"given":"Rafael Scaf de","family":"Molon","sequence":"additional","affiliation":[{"name":"Sao Paulo State University,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1210\/er.2018-00001","article-title":"Atypical Femur Fractures: Review of Epidemiology, Relationship to Bisphosphonates, Prevention, and Clinical Management","volume":"40","author":"Black DM","year":"2019","journal-title":"Endocr Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1530\/JOE-13-0040","article-title":"Alendronate promotes bone formation by inhibiting protein prenylation in osteoblasts in rat tooth replantation model","volume":"219","author":"Komatsu K","year":"2013","journal-title":"J Endocrinol"},{"key":"ref3","article-title":"A decade of bisphosphonate bone complications: what it has taught us about bone physiology","volume":"29","author":"Marx RE","year":"2014","journal-title":"Int J Oral Maxillofac Implants"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1002\/jbmr.2490","article-title":"OPG-Fc but Not Zoledronic Acid Discontinuation Reverses Osteonecrosis of the Jaws (ONJ) in Mice","volume":"30","author":"de Molon RS","year":"2015","journal-title":"J Bone Miner Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.bone.2016.06.011","article-title":"Osteonecrosis of the jaws (ONJ) in mice after extraction of teeth with periradicular disease","volume":"90","author":"Soundia A","year":"2016","journal-title":"Bone"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.bone.2014.07.027","article-title":"Spontaneous osteonecrosis of the jaws in the maxilla of mice on antiresorptive treatment: a novel ONJ mouse model","volume":"68","author":"de Molon RS","year":"2014","journal-title":"Bone"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/sj.ebd.6401037","article-title":"Review finds that severe periodontitis affects 11% of the world population","volume":"15","author":"Richards D","year":"2014","journal-title":"Evid Based Dent"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1902\/jop.2007.060390","article-title":"Effect of 17beta-Estradiol and Alendronate on the Removal Torque of Osseointegrated Titanium Implants in Ovariectomized Rats","volume":"78","author":"Giro G","year":"2007","journal-title":"J Periodontol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1177\/0022034512472335","article-title":"Effect of local vs. systemic bisphosphonate delivery on dental implant fixation in a model of osteonecrosis of the jaw","volume":"92","author":"Abtahi J","year":"2013","journal-title":"J Dent Res"},{"key":"ref10","first-page":"218","article-title":"Effects of alendronate on the removal torque of implants in rats with induced osteoporosis","volume":"18","author":"Narai S","year":"2003","journal-title":"Int J Oral Maxillofac Implants"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1600-0501.2010.01989.x","article-title":"The effect of oestrogen and alendronate therapies on postmenopausal bone loss around osseointegrated titanium implants","volume":"22","author":"Giro G","year":"2011","journal-title":"Clin Oral Implants Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1902\/jop.2005.76.1.107","article-title":"Alendronate therapy may be effective in the prevention of bone loss around titanium implants inserted in estrogen-deficient rats","volume":"76","author":"Duarte PM","year":"2005","journal-title":"J Periodontol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1111\/clr.12492","article-title":"Effects of the long-term administration of alendronate on the mechanical properties of the basal bone and on osseointegration","volume":"26","author":"Verzola MH","year":"2015","journal-title":"Clin Oral Implants Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1007\/s11999-016-4714-6","article-title":"Implant-delivered Alendronate Causes a Dose-dependent Response on Net Bone Formation Around Porous Titanium Implants in Canines","volume":"474","author":"Pura JA","year":"2016","journal-title":"Clin Orthop Relat Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s11999-013-3120-6","article-title":"Local alendronic acid elution increases net periimplant bone formation: a micro-CT analysis","volume":"472","author":"Bobyn JD","year":"2014","journal-title":"Clin Orthop Relat Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1002\/jbm.a.35602","article-title":"The effect of alendronate on biomineralization at the bone\/implant interface","volume":"104","author":"Karlsson J","year":"2016","journal-title":"J Biomed Mater Res A"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.ijom.2015.05.013","article-title":"Influence of the local application of sodium alendronate gel on osseointegration of titanium implants","volume":"44","author":"Guimaraes MB","year":"2015","journal-title":"Int J Oral Maxillofac Surg"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1097\/BLO.0b013e31813c6696","article-title":"Soaking morselized allograft in bisphosphonate can impair implant fixation","volume":"463","author":"Jakobsen T","year":"2007","journal-title":"Clin Orthop Relat Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1007\/s00784-018-2612-x","article-title":"Evaluation of bone turnover after bisphosphonate withdrawal and its influence on implant osseointegration: an in vivo study in rats","volume":"23","author":"Frizzera F","year":"2019","journal-title":"Clin Oral Investig"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"104779","DOI":"10.1016\/j.archoralbio.2020.104779","article-title":"Long-term evaluation of alendronate treatment on the healing of calvaria bone defects in rats. Biochemical, histological and immunohistochemical analyses","volume":"117","author":"de Molon RS","year":"2020","journal-title":"Arch Oral Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1016\/j.joms.2020.08.022","article-title":"Alendronate Impairs Healing of Calvaria Critical Defects After Bone Graft With Different Bone Substitute Materials","volume":"78","author":"Prisinoto NR","year":"2020","journal-title":"J Oral Maxillofac Surg"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.nut.2005.04.002","article-title":"Comparing rat's to human's age: how old is my rat in people years?","volume":"21","author":"Quinn R","year":"2005","journal-title":"Nutrition"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.bone.2011.04.022","article-title":"Bisphosphonates: the first 40 years","volume":"49","author":"Russell RG","year":"2011","journal-title":"Bone"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1359\/jbmr.070701","article-title":"Alendronate has an anabolic effect on bone through the differentiation of mesenchymal stem cells","volume":"22","author":"Duque G","year":"2007","journal-title":"J Bone Miner Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S8756-3282(00)00376-8","article-title":"Alendronate increases bone strength by increasing the mean degree of mineralization of bone tissue in osteoporotic women","volume":"27","author":"Boivin GY","year":"2000","journal-title":"Bone"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1007\/s00198-011-1804-x","article-title":"Pathophysiology of atypical femoral fractures and osteonecrosis of the jaw","volume":"22","author":"Compston J.","year":"2011","journal-title":"Osteoporos Int"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/0021-9290(90)90030-7","article-title":"Physical characteristics affecting the tensile failure properties of compact bone","volume":"23","author":"Currey JD","year":"1990","journal-title":"J Biomech"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1210\/jc.2004-0952","article-title":"Severely suppressed bone turnover: a potential complication of alendronate therapy","volume":"90","author":"Odvina CV","year":"2005","journal-title":"J Clin Endocrinol Metab"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jbmr.1998","article-title":"Atypical subtrochanteric and diaphyseal femoral fractures: second report of a task force of the American Society for Bone and Mineral Research","volume":"29","author":"Shane E","year":"2014","journal-title":"J Bone Miner Res"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/09243860500095471","article-title":"Microcracks colocalize within highly mineralized regions of cortical bone tissue","volume":"42","author":"Wasserman N","year":"2005","journal-title":"Eur J Morphol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bone.2013.01.016","article-title":"Effects of minodronic acid and alendronate on bone remodeling, microdamage accumulation, degree of mineralization and bone mechanical properties in ovariectomized cynomolgus monkeys","volume":"54","author":"Yamagami Y","year":"2013","journal-title":"Bone"}],"container-title":["Brazilian Dental Journal"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0103-64402024000100252&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T12:33:07Z","timestamp":1733142787000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0103-64402024000100252&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":31,"alternative-id":["S0103-64402024000100252"],"URL":"https:\/\/doi.org\/10.1590\/0103-644020235751","relation":{},"ISSN":["1806-4760","0103-6440"],"issn-type":[{"type":"electronic","value":"1806-4760"},{"type":"print","value":"0103-6440"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e24-5751"}}