{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,15]],"date-time":"2026-11-15T06:26:44Z","timestamp":1794724004005,"version":"4.9.1"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract The aim of this study was to verify the biomodifying action of 6.5% grape seed extract solutions, with different pH, when applied on dentin collagen. Dentin bars (1.7 mm x 6.0 mm x 0.5 mm) were demineralized for 5 hours in 10% phosphoric acid, and distributed into the following groups: acid solution (pH=4.42); neutral (pH=6.96); alkaline (pH=11.92) and distilled water (pH=6.75). Three-point flexural test (n=10) and mass variation (n=10) were assessed at different periods (baseline, after biomodification, 7 and 14 days of remineralizing solution storage). For qualitative analysis, similar dentin bars were prepared and analyzed using Fourier transform infrared spectroscopy (FT-IR) and Raman Spectroscopy (FT-Raman) before and after 1 hour of immersion in biomodifying solution with different pH. Data were subjected to Shapiro-Wilk normality tests, followed by two-way ANOVA for repeated measures and Tukey's post-test (p&lt;0.05). Alkaline solution was effective in increasing the modulus of elasticity, showing a decrease after 7 days and subsequent stabilization after 14 days of storage. Acid solution group showed a greater increase in the modulus of elasticity immediately after biomodification, but it was not stable over storage. Regarding mass variation, only the acid solution showed an increase in mass after biomodification and 14 days of storage. In relation to FT-IR and FT-Raman, all solutions showed interaction with collagen at some level. Therefore, pH of the solution directly influences the action of the grape seed extract, with satisfactory results being found in both acidic and alkaline solutions.<\/jats:p>","DOI":"10.1590\/0103-6440202406048","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T12:06:14Z","timestamp":1734350774000},"source":"Crossref","is-referenced-by-count":3,"title":["Does the pH of the grape seed extract interfere in the biomodification capacity of dentin collagen?"],"prefix":"10.1590","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2993-534X","authenticated-orcid":false,"given":"Marcelo Victor Sidou","family":"Lemos","sequence":"first","affiliation":[{"name":"Federal University of Cear\u00e1,  Brazil; University of Fortaleza,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6077-3476","authenticated-orcid":false,"given":"Gabriela Ara\u00fajo","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Chillavert Dias","family":"Pascoal","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1774-9862","authenticated-orcid":false,"given":"Talita Arrais Daniel","family":"Mendes","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1,  Brazil; University of Fortaleza,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6808-092X","authenticated-orcid":false,"given":"S\u00e9rgio Lima","family":"Santiago","sequence":"additional","affiliation":[{"name":"Federal University of Cear\u00e1,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.1100894","article-title":"Effect of collagen cross-linkers on dentin bond strength: A systematic review and network meta-analysis","volume":"10","author":"Chen H","year":"2023","journal-title":"Front. Bioeng. Biotechnol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.dental.2016.03.008","article-title":"Collagen cross-linkers on dentin bonding: Stability of the adhesive interfaces, degree of conversion of the adhesive, cytotoxicity and in situ MMP inhibition","volume":"32","author":"Hass V","year":"2016","journal-title":"Dent. Mater"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"12","DOI":"10.2341\/20-138-L","article-title":"Evaluation of Novel Plant-derived Monomers-based Pretreatment on Bonding to Sound and Caries-affected Dentin","volume":"47","author":"Lemos M","year":"2022","journal-title":"Oper. Dent"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1177\/0022034514523783","article-title":"Dentin Biomodification Potential Depends on Polyphenol Source","volume":"93","author":"Aguiar TRR","year":"2014","journal-title":"J. Dent. Res"},{"issue":"24","key":"ref5","first-page":"125","article-title":"Prodelphinidins enhance dentin matrix properties and promote adhesion to methacrylate resin","volume":"24","author":"Reis-Havlat M","year":"2024","journal-title":"Dent Mater"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jdent.2018.12.012","article-title":"Two-year clinical evaluation of proanthocyanidins added to a two-step etch-and-rinse adhesive","volume":"81","author":"Souza LC de","year":"2019","journal-title":"J. Dent"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1177\/0022034516680586","article-title":"Biostability of the Proanthocyanidins-Dentin Complex and Adhesion Studies","volume":"96","author":"Leme-Kraus AA","year":"2017","journal-title":"J. Dent. Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3288","DOI":"10.1016\/j.actbio.2014.03.036","article-title":"Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins","volume":"10","author":"Vidal CMP","year":"2014","journal-title":"Acta Biomater"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.4012\/dmj.2017-062","article-title":"Dual function of proanthocyanidins as both MMP inhibitor and crosslinker in dentin biomodification: A literature review","volume":"37","author":"Balalaie A","year":"2018","journal-title":"Dent. Mater. J"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.dental.2013.10.012","article-title":"Dentin biomodification: strategies, renewable resources and clinical applications","volume":"30","author":"Bedran-Russo AK","year":"2014","journal-title":"Dent. Mater"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.dental.2012.08.004","article-title":"Optimizing dentin bond durability: Control of collagen degradation by matrix metalloproteinases and cysteine cathepsins","volume":"29","author":"Tj\u00e4derhane L","year":"2013","journal-title":"Dent. Mater"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.dental.2013.01.013","article-title":"Enhancement in dentin collagen\u2019s biological stability after proanthocyanidins treatment in clinically relevant time periods","volume":"29","author":"Liu Y","year":"2013","journal-title":"Dent. Mater"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1007\/s00217-014-2375-9","article-title":"Comparison of the degradation kinetics of A-type and B-type proanthocyanidins dimers as a function of pH and temperature","volume":"240","author":"Xu Z","year":"2015","journal-title":"Eur. Food Res. 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Mater"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.fitote.2014.12.006","article-title":"A galloylated dimeric proanthocyanidin from grape seed exhibits dentin biomodification potential","volume":"101","author":"Phansalkar RS","year":"2015","journal-title":"Fitoterapia"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1177\/0022034519892959","article-title":"Dimeric Proanthocyanidins on the Stability of Dentin and Adhesive Biointerfaces","volume":"99","author":"Leme-Kraus AA","year":"2020","journal-title":"J. Dent. Res"},{"key":"ref19","doi-asserted-by":"crossref","DOI":"10.1111\/iej.13019","article-title":"Direct and transdentinal biostimulatory effects of grape seed extract rich in proanthocyanidin on pulp cells","author":"dos Santos AF","year":"2019","journal-title":"Int. Endod. 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