{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:03:15Z","timestamp":1753887795714,"version":"3.41.2"},"reference-count":22,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Resumo Este estudo in vitro teve como objetivo investigar o efeito da nitreta\u00e7\u00e3o por implanta\u00e7\u00e3o i\u00f4nica de plasma nas propriedades de superf\u00edcie do tit\u00e2nio e na resposta celular inicial. Discos de tit\u00e2nio grau 4 (12,7 \u00d7 2 mm) foram jateados com part\u00edculas de \u00f3xido de alum\u00ednio para criar superf\u00edcies moderadamente rugosas. Os discos experimentais (TiN) foram nitrados usando a t\u00e9cnica de implanta\u00e7\u00e3o i\u00f4nica de plasma em uma c\u00e2mara de v\u00e1cuo. A caracteriza\u00e7\u00e3o da superf\u00edcie foi realizada usando microscopia confocal a laser, microscopia de for\u00e7a at\u00f4mica (AFM) e espectroscopia de fotoel\u00e9trons de raios X (XPS). A molhabilidade da superf\u00edcie foi avaliada medindo o \u00e2ngulo de contato de uma gota s\u00e9ssil usando um goni\u00f4metro. C\u00e9lulas osteobl\u00e1sticas humanas foram semeadas nos discos para avaliar a ades\u00e3o e prolifera\u00e7\u00e3o celular nos dias 1, 3, 5 e 7 de cultura, usando um ensaio de composto de tetraz\u00f3lio. A atividade da fosfatase alcalina (ALP) foi medida no dia 7 para avaliar a diferencia\u00e7\u00e3o celular. A morfologia celular foi examinada por microscopia eletr\u00f4nica de varredura (SEM) e microscopia confocal a laser. O grupo TiN exibiu micro-rugosidade similar ao grupo controle; no entanto, apresentou maior densidade de nanostruturas, aumento do conte\u00fado de nitrog\u00eanio e ligeira melhoria na molhabilidade. A prolifera\u00e7\u00e3o celular e a atividade da ALP foram similares entre os grupos ap\u00f3s sete dias de cultura. Em conclus\u00e3o, a nitreta\u00e7\u00e3o por implanta\u00e7\u00e3o i\u00f4nica de plasma melhora as nanocaracter\u00edsticas da superf\u00edcie e a molhabilidade sem comprometer a biocompatibilidade do tit\u00e2nio, tornando-se uma t\u00e9cnica promissora de modifica\u00e7\u00e3o de superf\u00edcie para implantes dent\u00e1rios e ortop\u00e9dicos.<\/jats:p>","DOI":"10.1590\/0103-6440202406111","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T10:43:59Z","timestamp":1729853039000},"source":"Crossref","is-referenced-by-count":0,"title":["Nitriding Titanium by Plasma Ion Implantation: Surface Properties and Initial Osteoblast Cell Response"],"prefix":"10.1590","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0743-5376","authenticated-orcid":false,"given":"Plinio Mendes","family":"Senna","sequence":"first","affiliation":[{"name":"State University of Rio de Janeiro,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5775-0222","authenticated-orcid":false,"given":"Carlos Fernando","family":"Mour\u00e3o","sequence":"additional","affiliation":[{"name":"Tufts University School of Dental Medicine,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1819-1231","authenticated-orcid":false,"given":"Cindy Goes","family":"Dodo","sequence":"additional","affiliation":[{"name":"Marquette University,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1371-0186","authenticated-orcid":false,"given":"James L.","family":"Rutkowski","sequence":"additional","affiliation":[{"name":"Jacksonville University,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4329-0437","authenticated-orcid":false,"given":"Altair A. Del Bel","family":"Cury","sequence":"additional","affiliation":[{"name":"Piracicaba Dental School,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1586\/erd.09.74","article-title":"Improving osseointegration of dental implants","volume":"7","author":"Elias CN","year":"2010","journal-title":"Expert review of medical devices"},{"issue":"1","key":"ref2","first-page":"63","article-title":"On implant surfaces: a review of current knowledge and opinions","volume":"25","author":"Wennerberg A","year":"2010","journal-title":"The International journal of oral & maxillofacial implants"},{"issue":"17","key":"ref3","doi-asserted-by":"crossref","first-page":"3757","DOI":"10.1016\/S0142-9612(02)00115-1","article-title":"Effects of Ti ions and particles on neutrophil function and morphology","volume":"23","author":"Kumazawa R","year":"2002","journal-title":"Biomaterials"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1111\/cid.12167","article-title":"Surface Damage on Dental Implants with Release of Loose Particles after Insertion into Bone","volume":"17","author":"Senna P","year":"2015","journal-title":"Clin Implant Dent Relat Res"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1111\/j.1600-0714.2010.00958.x","article-title":"Mapping of titanium particles in peri-implant oral mucosa by laser ablation inductively coupled plasma mass spectrometry and high-resolution optical darkfield microscopy","volume":"40","author":"Flatebo RS","year":"2011","journal-title":"Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology"},{"issue":"7","key":"ref6","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1902\/jop.2006.050406","article-title":"Host response to titanium dental implant placement evaluated in a human oral model","volume":"77","author":"Flatebo RS","year":"2006","journal-title":"Journal of periodontology"},{"issue":"14","key":"ref7","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/j.biomaterials.2004.05.017","article-title":"Expression of inflammatory cytokines, RANKL and OPG induced by titanium, cobalt-chromium and polyethylene particles","volume":"26","author":"Masui T","year":"2005","journal-title":"Biomaterials"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/jbm.a.30557","article-title":"Mesenchymal stem cell adhesion and spreading on microwave plasma-nitrided titanium alloy","volume":"76","author":"Clem WC","year":"2006","journal-title":"Journal of biomedical materials research Part A"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/0142-9612(96)83289-3","article-title":"Cytocompatibility of Ti-6Al-4V and Ti-5Al-2.5Fe alloys according to three surface treatments, using human fibroblasts and osteoblasts","volume":"17","author":"Bordji K","year":"1996","journal-title":"Biomaterials"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1016\/j.rinma.2020.100071","article-title":"Characterization of surface layer formed by gas blow induction heating nitriding at different temperatures and its effect on the fatigue properties of titanium alloy","volume":"5","author":"Takesue S","year":"2020","journal-title":"Results in Materials"},{"issue":"10","key":"ref11","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.3390\/ma16103609","article-title":"Structure and wear performance of a titanium alloy by using low-temperature plasma oxy-nitriding","volume":"16","author":"Li H","year":"2023","journal-title":"Materials"},{"key":"ref12","article-title":"Effect of plasma-nitrided titanium on mechanical properties and initial cell adhesion","author":"Krasaesin A","year":"2023","journal-title":"Materials Today: Proceedings"},{"issue":"2","key":"ref13","first-page":"237","article-title":"Osteoblastlike cell adhesion on titanium surfaces modified by plasma nitriding","volume":"26","author":"da Silva JS","year":"2011","journal-title":"The International journal of oral & maxillofacial implants"},{"key":"ref14","article-title":"The effect of plasma-nitrided titanium surfaces on osteoblastic cell adhesion, proliferation, and differentiation","author":"Ferraz EP","year":"2013","journal-title":"Journal of biomedical materials research Part A"},{"issue":"4","key":"ref15","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1600-0501.2011.02352.x","article-title":"A novel technique for tailored surface modification of dental implants - 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