{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T01:40:18Z","timestamp":1773538818376,"version":"3.50.1"},"reference-count":50,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Foundation for Research Support of the State of S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2017\/06537-7"],"award-info":[{"award-number":["2017\/06537-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Abstract Different diluents are used to prepare toothpaste slurries in vitro and in situ erosive tooth wear studies. This study aimed to evaluate the influence of diluents on the anti-erosive potential of toothpastes with different active agents. Bovine enamel specimens were assigned to groups (n = 12) according to slurry type (in vitro deionized water, in vitro remineralizing solution, clarified human saliva, or in situ human saliva) and toothpaste formulation: placebo (0 ppm F), sodium monofluorophosphate (1450 ppm F - MFP), sodium fluoride (1450 ppm F - NaF), stannous fluoride + sodium fluoride (1100 ppm F SnF\u2082 + 350 ppm F NaF - NaF\/SnF\u2082), or chitosan (0.5%) + sodium fluoride (700 ppm F) + amine fluoride (700 ppm F) - Chi\/NaF\/AmF. A 5-day erosion cycle was performed, intercalating four erosive challenges (1 min, 1% citric acid, pH 3.5) and two remineralizing treatments, applied before the first and after the last erosive challenge each day (brushing for 15 seconds and immersion in slurry for up to 2 minutes). Surface microhardness (SMH) and tissue loss by contact profilometry were measured, and percentage SMH loss (%SMH) was calculated. Across all toothpastes, in situ human saliva resulted in the highest SMH and lowest tissue loss. Under in vitro conditions, NaF\/SnF\u2082 and Chi\/NaF\/AmF slurries with human saliva yielded the best outcomes. For other formulations, no significant differences were observed between in vitro diluents. Results indicate that the diluent type influences SMH and profilometry outcomes, depending on the active agents. Toothpaste containing Sn, chitosan, and fluoride showed the most significant anti-erosive potential.<\/jats:p>","DOI":"10.1590\/0103-644020256448","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T15:03:24Z","timestamp":1763737404000},"source":"Crossref","is-referenced-by-count":1,"title":["Influence of Slurry Diluents on Toothpaste Efficacy Against Erosive Tooth Wear: In Vitro and In Situ Studies"],"prefix":"10.1590","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6234-8913","authenticated-orcid":false,"given":"Laura Nobre","family":"Ferraz","sequence":"first","affiliation":[{"name":"University of Araras,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4022-0342","authenticated-orcid":false,"given":"Isabele","family":"Vieira","sequence":"additional","affiliation":[{"name":"University of Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5441-1997","authenticated-orcid":false,"given":"Gabriel Candolato de","family":"Almeida","sequence":"additional","affiliation":[{"name":"University of Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6376-679X","authenticated-orcid":false,"given":"Tatiane Cristina","family":"Dotta","sequence":"additional","affiliation":[{"name":"University of Araras,  Brazil; University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8226-3100","authenticated-orcid":false,"given":"Waldemir Francisco","family":"Vieira-Junior","sequence":"additional","affiliation":[{"name":"S\u00e3o Leopoldo Mandic Institute and Dental Research Center,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8604-3201","authenticated-orcid":false,"given":"N\u00fabia Inoc\u00eancya Pavesi","family":"Pini","sequence":"additional","affiliation":[{"name":"Uning\u00e1 University Center,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5950-6370","authenticated-orcid":false,"given":"Gl\u00e1ucia Maria Bovi","family":"Ambrosano","sequence":"additional","affiliation":[{"name":"University of Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5457-3347","authenticated-orcid":false,"given":"D\u00e9bora Alves Nunes Leite","family":"Lima","sequence":"additional","affiliation":[{"name":"University of Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"120","key":"ref1","first-page":"1","article-title":"Protective effect of fluorides on erosion and erosion\/abrasion in enamel: a systematic review and meta-analysis of randomized in situ trials","volume":"1","author":"Zanatta RF","year":"2020","journal-title":"Arch Oral Biol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/j.1600-0722.2008.00575.x","article-title":"The Effect of Human Saliva Substitutes in an Erosion-Abrasion Cycling Model","volume":"116","author":"Hara AT","year":"2008","journal-title":"Eur J Oral Sci"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1590\/S1678-77572010000400002","article-title":"pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations","volume":"18","author":"Buzalaf 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Deposition and retention on pellicle-coated enamel","volume":"64","author":"Khambe D","year":"2014","journal-title":"Int Dent J."},{"issue":"1","key":"ref45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-41736-7","article-title":"Effect of fluoride mouthrinses and stannous ions on the erosion protective properties of the in situ pellicle","volume":"9","author":"Kensche A","year":"2019","journal-title":"Sci Rep"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1600-0722.2007.00454.x","article-title":"Chitosan adsorption to salivary pellicles","volume":"115","author":"Van Der Mei HC","year":"2007","journal-title":"Eur J Oral Sci"},{"issue":"3","key":"ref47","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.jdent.2011.12.012","article-title":"Chitosan microparticles for the controlled delivery of fluoride","volume":"40","author":"Keegan GM","year":"2012","journal-title":"J Dent"},{"issue":"8","key":"ref48","doi-asserted-by":"crossref","first-page":"3814","DOI":"10.1021\/la702653m","article-title":"Interactions between chitosan and SDS at a low-charged silica substrate compared to interactions in the bulk - The effect of ionic strength","volume":"24","author":"Lundin M","year":"2008","journal-title":"Langmuir"},{"issue":"1","key":"ref49","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1159\/000112816","article-title":"The protective effect of tif4, SnF2 and NaF against erosion-like lesions in situ","volume":"42","author":"Hove LH","year":"2008","journal-title":"Caries Res"},{"issue":"6954","key":"ref50","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nature01970","article-title":"Lubrication by charged polymers","volume":"425","author":"Raviv U","year":"2003","journal-title":"Nature"}],"container-title":["Brazilian Dental Journal"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0103-64402025000100244&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T15:03:33Z","timestamp":1763737413000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0103-64402025000100244&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":50,"alternative-id":["S0103-64402025000100244"],"URL":"https:\/\/doi.org\/10.1590\/0103-644020256448","relation":{},"ISSN":["1806-4760","0103-6440"],"issn-type":[{"value":"1806-4760","type":"electronic"},{"value":"0103-6440","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e25-6448"}}