{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:05:03Z","timestamp":1764601503218,"version":"3.46.0"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"State of S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2016\/11461-7"],"award-info":[{"award-number":["2016\/11461-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"abstract":"<jats:p>Resumo Cimento de aluminato de c\u00e1lcio (CAC) tem sido proposto como alternativa ao agregado de tri\u00f3xido mineral (MTA), por\u00e9m seu comportamento biol\u00f3gico permanece pouco explorado. Este estudo avaliou a citotoxicidade, a genotoxicidade e a express\u00e3o de genes pr\u00f3-inflamat\u00f3rios em fibroblastos expostos a formula\u00e7\u00f5es de CAC contendo \u00f3xido de zinco ou \u00f3xido de bismuto, com diferentes propor\u00e7\u00f5es de cloreto de c\u00e1lcio, em compara\u00e7\u00e3o ao MTA. Os fibroblastos foram cultivados com CACz (25% ZnO + 2,8% CaCl\u2082), CACb (25% Bi\u2082O\u2083 + 2,8% CaCl\u2082), CACb+ (25% Bi\u2082O\u2083 + 10% CaCl\u2082) ou MTA; c\u00e9lulas n\u00e3o expostas serviram como controles. A citotoxicidade foi avaliada por morfologia em fluoresc\u00eancia e ensaio de viabilidade MTT em 24 h e 72 h; a genotoxicidade, pelo ensaio do cometa em 24 h; e a express\u00e3o g\u00eanica de COL-1, TNF-\u03b1, IL-1\u03b2, IL-6 e MMP-9 por RT-qPCR em 72 h. Os dados foram analisados pelo teste de Kruskal-Wallis, seguido do p\u00f3s-teste de Student-Newman-Keuls (\u03b1 = 0,05). A viabilidade celular n\u00e3o diferiu entre os grupos em 24 h, mas foi significativamente maior para o CACb+ em 72 h (p &lt; 0,05). Os n\u00edveis de dano ao DNA foram semelhantes entre os grupos (p &gt; 0,05). Quanto \u00e0 express\u00e3o g\u00eanica, n\u00e3o foram observadas diferen\u00e7as significativas para COL-1, IL-1\u03b2 ou TNF-\u03b1. Todos os cimentos aumentaram significativamente a express\u00e3o de IL-6 em compara\u00e7\u00e3o ao controle (p &lt; 0,05), com os maiores n\u00edveis no MTA e os menores no CACb+. O CACb+ aumentou significativamente a express\u00e3o de MMP-9 (p &lt; 0,05), enquanto o CACz apresentou os menores n\u00edveis. Dentro das limita\u00e7\u00f5es deste estudo, as formula\u00e7\u00f5es de CAC com aditivos n\u00e3o foram genot\u00f3xicas nem citot\u00f3xicas, e seu comportamento biol\u00f3gico foi dependente da composi\u00e7\u00e3o, sendo que o CACb+ demonstrou o perfil mais favor\u00e1vel ao potencializar a viabilidade de fibroblastos e o remodelamento da matriz extracelular.<\/jats:p>","DOI":"10.1590\/0103-644020256511","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:02:22Z","timestamp":1764601342000},"source":"Crossref","is-referenced-by-count":0,"title":["Cyto-genotoxicity and immunomodulation in fibroblasts exposed to calcium-aluminate cement with different radiopacifiers"],"prefix":"10.1590","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7034-9772","authenticated-orcid":false,"given":"Ant\u00f4nio Secco","family":"Martorano","sequence":"first","affiliation":[{"name":"University of S\u00e3o Paulo,  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de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"University of Vale do Para\u00edba,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2614-2122","authenticated-orcid":false,"given":"Walter","family":"Raucci-Neto","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6662-0486","authenticated-orcid":false,"given":"Paulo Tambasco de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9048-9935","authenticated-orcid":false,"given":"Larissa Moreira Spinola de","family":"Castro-Raucci","sequence":"additional","affiliation":[{"name":"University of Ribeir\u00e3o Preto,  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agents","volume":"37","author":"Aguilar FG","year":"2011","journal-title":"J Endod"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/j.1365-2591.2010.01770.x","article-title":"Chemical, physical, and mechanical properties of a novel calcium aluminate endodontic cement","volume":"43","author":"Oliveira IR","year":"2010","journal-title":"Int Endod J"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1111\/iej.12883","article-title":"Calcium chloride-enriched calcium aluminate cement promotes in vitro osteogenesis","volume":"51","author":"Castro-Raucci LMS","year":"2018","journal-title":"Int Endod J"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.1590\/1678-7757-2019-0105","article-title":"Impact of calcium aluminate cement with additives on dental pulp-derived cells","volume":"28","author":"Messias NS","year":"2020","journal-title":"J Appl Oral Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.archoralbio.2018.04.018","article-title":"Sources of free radicals and oxidative stress in the oral cavity","volume":"92","author":"\u017bukowski P","year":"2018","journal-title":"Arch Oral Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1177\/10454411980090040701","article-title":"eriapical inflammatory responses and their modulation","volume":"9","author":"Stashenko P","year":"1998","journal-title":"Crit Rev Oral Biol Med"},{"issue":"10","key":"ref9","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/j.1365-2591.2010.01747.x","article-title":"Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide","volume":"43","author":"Zeferino EG","year":"2010","journal-title":"Int Endod 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Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1097\/01.DON.0000121614.10075.A3","article-title":"Lack of genotoxicity of formocresol, paramonochlorophenol, and calcium hydroxide on mammalian cells by comet assay","volume":"30","author":"Ribeiro DA","year":"2004","journal-title":"J Endod"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.tripleo.2005.02.080","article-title":"Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro","volume":"101","author":"Ribeiro DA","year":"2006","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/j.1365-2591.2012.02038.x","article-title":"Evaluation of cytotoxicity and up-regulation of gelatinases in fibroblast cells by three root repair materials","volume":"45","author":"Silva EJNL","year":"2012","journal-title":"Int Endod J"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1055\/s-0040-1713073","article-title":"Mineral Trioxide Aggregate Applications in Endodontics: A Review","volume":"14","author":"Cervino G","year":"2020","journal-title":"Eur J Dent"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1097\/00004770-200401000-00005","article-title":"Attachment and morphological behavior of human periodontal ligament fibroblasts to mineral trioxide aggregate: A scanning electron microscope study","volume":"30","author":"Balto HA","year":"2004","journal-title":"J Endod"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1111\/iej.12791","article-title":"Influence of early mineral deposits of silicate- and aluminate-based cements on push-out bond strength to root dentine","volume":"51","author":"do Carmo SS","year":"2018","journal-title":"Int Endod J"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.joen.2011.11.002","article-title":"Biocompatibility of new calcium aluminate cement (EndoBinder)","volume":"38","author":"Aguilar FG","year":"2012","journal-title":"J Endod"},{"key":"ref27","series-title":"IntechOpen","first-page":"9","volume-title":"Cytokine Profiling Plays a Crucial Role in Activating Immune System to Clear Infectious Pathogens","author":"Mu\u00f1oz-Carrillo JL","year":"2019"},{"issue":"2","key":"ref28","article-title":"Interleukin-6: Discovery of a pleiotropic cytokine","volume":"8","author":"Kishimoto T","year":"2006","journal-title":"Arthritis Res Ther"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.4049\/jimmunol.157.6.2618","article-title":"IL-6 is an in vitro and in vivo autocrine growth factor for middle T antigen-transformed endothelial cells","volume":"157","author":"Giraudo E","year":"1996","journal-title":"J Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/rheumatology\/34.4.321","article-title":"Interleukin-6 (IL-6) induces the proliferation of synovial fibroblastic cells in the presence of soluble IL-6 receptor","volume":"34","author":"Mihara M","year":"1995","journal-title":"Rheumatology"},{"key":"ref31","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M405194200","article-title":"Calcium-induced matrix metalloproteinase 9 gene expression is differentially regulated by ERK1\/2 and p38 MAPK in oral keratinocytes and oral squamous cell carcinoma","volume":"279","author":"Mukhopadhyay S","year":"2004","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1152\/physiol.00029.2013","article-title":"Matrix metalloproteinase-9: Many shades of function in cardiovascular disease","volume":"28","author":"Yabluchanskiy 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