{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:50:00Z","timestamp":1789188600030,"version":"build-2803163510"},"reference-count":40,"publisher":"FapUNIFESP (SciELO)","issue":"5","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz J Med Biol Res"],"DOI":"10.1590\/1414-431x20186754","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:03:09Z","timestamp":1521936189000},"source":"Crossref","is-referenced-by-count":8,"title":["Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion"],"prefix":"10.1590","volume":"51","author":[{"given":"A.","family":"Paim","sequence":"first","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul,  Brasil; Universidade Federal do Rio Grande do Sul,  Brasil; Universidade Federal do Rio Grande do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.I.","family":"Braghirolli","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.S.M.","family":"Cardozo","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Pranke","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul,  Brasil; Instituto de Pesquisa com C\u00e9lulas-Tronco,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.C.","family":"Tessaro","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2018]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1002\/term.1978","article-title":"A review of key challenges of electrospun scaffolds for tissue-engineering applications","volume":"10","author":"Khorshidi S","year":"2016","journal-title":"J Tissue Eng Regen Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1016\/j.actbio.2011.02.036","article-title":"Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration","volume":"7","author":"Vaquette C","year":"2011","journal-title":"Acta Biomater"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1016\/j.actbio.2010.02.006","article-title":"Osteogenic differentiation of bone marrow stromal cells on poly(\u03b5-caprolactone) nanofiber scaffolds","volume":"6","author":"Ruckh TT","year":"2010","journal-title":"Acta Biomater"},{"key":"ref4","series-title":"Electrospun nanofibrous poly(\u03b5-caprolactone) (PCL) scaffolds for liver tissue engineering","author":"Gluck JM","year":"2007"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1021\/bm060680j","article-title":"Electrospun Poly(\u03b5-caprolactone) microfiber and multilayer nanofiber\/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration","volume":"7","author":"Pham QP","year":"2006","journal-title":"Biomacromolecules"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1089\/107632703322066723","article-title":"Design of a flow perfusion bioreactor system for bone tissue-engineering applications","volume":"9","author":"Bancroft GN","year":"2003","journal-title":"Tissue Eng"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"5490","DOI":"10.3390\/ma8085259","article-title":"Influence of different three-dimensional open porous titanium scaffold designs on human osteoblasts behavior in static and dynamic cell investigations","volume":"8","author":"Markhoff J","year":"2015","journal-title":"Materials"},{"key":"ref8","series-title":"Tubular perfusion system bioreactor for the dynamic culture of human mesenchymal stem cells","author":"Yeatts AB","year":"2012"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1089\/ten.tec.2015.0016","article-title":"Effects of stirring and fluid perfusion on the in vitro degradation of calcium phosphate cement\/PLGA composites","volume":"21","author":"An J","year":"2015","journal-title":"Tissue Eng Part C Methods"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1089\/10763270360728107","article-title":"Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro","volume":"9","author":"Cartmell SH","year":"2003","journal-title":"tissue Eng"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.msec.2015.09.034","article-title":"An axial distribution of seeding, proliferation, and osteogenic differentiation of MC3T3-E1 cells and rat bone marrow-derived mesenchymal stem cells across a 3D Thai silk fibroin\/gelatin\/hydroxyapatite scaffold in a perfusion bioreactor","volume":"58","author":"Sinlapabodin S","year":"2016","journal-title":"Mater Sci Eng C"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1073\/pnas.0505661103","article-title":"In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation","volume":"103","author":"Datta N","year":"2006","journal-title":"Proc Natl Acad Sci"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1089\/ten.tea.2007.0255","article-title":"Effects of initial seeding density and fluid perfusion rate on formation of tissue-engineered bone","volume":"14","author":"Grayson WL","year":"2008","journal-title":"Tissue Eng Part A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1016\/j.jbiomech.2008.04.001","article-title":"Influence of perfusion and cyclic compression on proliferation and differentiation of bone marrow stromal cells in 3-dimensional culture","volume":"41","author":"Jagodzinski M","year":"2008","journal-title":"J Biomech"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s00784-015-1477-5","article-title":"Carious deciduous teeth are a potential source for dental pulp stem cells","volume":"20","author":"Werle SB","year":"2016","journal-title":"Clin Oral Investig"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"10304","DOI":"10.1073\/pnas.1506684112","article-title":"Flow perfusion effects on three-dimensional culture and drug sensitivity of Ewing sarcoma","volume":"112","author":"Santoro M","year":"2015","journal-title":"Proc Natl Acad Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1002\/jbm.a.35505","article-title":"Cell seeding density is a critical determinant for copolymer scaffolds-induced bone regeneration","volume":"103","author":"Yassin MA","year":"2015","journal-title":"J Biomed Mater Res Part A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"6307","DOI":"10.1039\/C6TB01377E","article-title":"Enhanced adhesion and differentiation of human mesenchymal stem cell inside apatite-mineralized\/poly(dopamine)-coated poly(\u03b5-caprolactone) scaffolds by stereolithography","volume":"4","author":"Cheng Y-L","year":"2016","journal-title":"J Mater Chem B"},{"key":"ref19","first-page":"279","author":"Kafi MA","year":"2014"},{"key":"ref20","series-title":"Development of 3d-printed biodegradable composite scaffolds for tissue engineering applications","author":"Serra T","year":"2014"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3389\/fbioe.2016.00041","article-title":"Human mesenchymal stem cell morphology and migration on microtextured titanium","volume":"4","author":"Banik BL","year":"2016","journal-title":"Front Bioeng Biotechnol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"293","DOI":"10.4161\/cam.24804","article-title":"Predicting how cells spread and migrate","volume":"7","author":"Kim D-H","year":"2013","journal-title":"Cell Adh Migr"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4498","DOI":"10.1039\/b924304f","article-title":"Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay","volume":"12","author":"Christophis C","year":"2010","journal-title":"Phys Chem Chem Phys"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1089\/ten.tea.2007.0294","article-title":"Tailoring fiber diameter in electrospun poly(\u03b5-Caprolactone) scaffolds for optimal cellular infiltration in cardiovascular tissue engineering","volume":"15","author":"Balguid A","year":"2009","journal-title":"Tissue Eng Part A"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.addr.2014.08.009","article-title":"Biomechanical forces in the skeleton and their relevance to bone metastasis: Biology and engineering considerations","volume":"79–80","author":"Lynch ME","year":"2014","journal-title":"Adv Drug Deliv Rev"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.5507\/bp.2009.005","article-title":"Dental pulp stem cells and their characterization","volume":"153","author":"Suchanek J","year":"2009","journal-title":"Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/1477-5956-10-46","article-title":"Changes in the proteomic profile of adipose tissue-derived mesenchymal stem cells during passages","volume":"10","author":"Capra E","year":"2012","journal-title":"Proteome Sci"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1002\/jor.20402","article-title":"Donor variation and loss of multipotency during in vitro expansion of human mesenchymal stem cells for bone tissue engineering","volume":"25","author":"Siddappa R","year":"2007","journal-title":"J Orthop Res"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.bej.2015.06.018","article-title":"Characterization of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development","volume":"108","author":"Heathman TRJ","year":"2016","journal-title":"Biochem Eng J"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"18279","DOI":"10.1038\/srep18279","article-title":"Comparison of the depolarization response of human mesenchymal stem cells from different donors","volume":"5","author":"Sundelacruz S","year":"2015","journal-title":"Sci Rep"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.joen.2010.02.020","article-title":"Expression of extracellular matrix proteins in human dental pulp stem cells depends on the donor tooth conditions","volume":"36","author":"Harumi Miyagi SP","year":"2010","journal-title":"J Endod"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s10495-005-0816-4","article-title":"A novel time resolved fluorometric assay of anoikis using Europium-labelled Annexin V in cultured adherent cells","volume":"10","author":"Engbers-Buijtenhuijs P","year":"2005","journal-title":"Apoptosis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/1350-4533(94)90077-9","article-title":"Fluid shear induced endothelial cell detachment from glass - influence of adhesion time and shear stress","volume":"16","author":"van Kooten TG","year":"1994","journal-title":"Med Eng Phys"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1089\/ten.tea.2009.0242","article-title":"Role of nanofibrous poly(Caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering-response to osteogenic regulators","volume":"16","author":"Binulal NS","year":"2010","journal-title":"Tissue Eng Part A"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1089\/ten.teb.2010.0370","article-title":"Influence of shear stress in perfusion bioreactor cultures for the development of three-dimensional bone tissue constructs: a review","volume":"16","author":"McCoy RJ","year":"2010","journal-title":"Tissue Eng Part B Rev"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"8158","DOI":"10.1021\/ie051144v","article-title":"Mathematical modeling of three-dimensional cell cultures in perfusion bioreactors","volume":"45","author":"Coletti F","year":"2006","journal-title":"Ind Eng Chem Res"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1002\/bit.20367","article-title":"Role of nutrient supply on cell growth in bioreactor design for tissue engineering of hematopoietic cells","volume":"89","author":"Pathi P","year":"2005","journal-title":"Biotechnol Bioeng"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s10439-006-9244-z","article-title":"Flow perfusion improves seeding of tissue engineering scaffolds with different architectures","volume":"35","author":"Alvarez-Barreto JF","year":"2007","journal-title":"Ann Biomed Eng"},{"key":"ref39","first-page":"215","article-title":"The effect of hydrodynamic shear on 3D engineered chondrocyte systems subject to direct perfusion","volume":"43","author":"Raimondi MT","year":"2006","journal-title":"Biorheology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1007\/s13770-016-9047-8","article-title":"The effect of polyurethane scaffold surface treatments on the adhesion of chondrocytes subjected to interstitial perfusion culture","volume":"13","author":"Raimondi MT","year":"2016","journal-title":"Tissue Eng Regen Med"}],"container-title":["Brazilian Journal of Medical and Biological Research"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0100-879X2018000500615&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T03:33:47Z","timestamp":1714707227000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0100-879X2018000500615&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":40,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2018]]}},"alternative-id":["S0100-879X2018000500615"],"URL":"https:\/\/doi.org\/10.1590\/1414-431x20186754","relation":{},"ISSN":["1414-431X","0100-879X"],"issn-type":[{"value":"1414-431X","type":"electronic"},{"value":"0100-879X","type":"print"}],"subject":[],"published":{"date-parts":[[2018]]},"article-number":"e6754"}}