{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:06:27Z","timestamp":1790834787369,"version":"4.1.0"},"reference-count":35,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Sciatic nerve transection (SNT), a model for studying neuropathic pain, mimics the clinical symptoms of \u201cphantom limb\u201d, a pain condition that arises in humans after amputation or transverse spinal lesions. In some vertebrate tissues, this condition decreases acetylcholinesterase (AChE) activity, the enzyme responsible for fast hydrolysis of released acetylcholine in cholinergic synapses. In spinal cord of frog Rana pipiens, this enzyme\u2019s activity was not significantly changed in the first days following ventral root transection, another model for studying neuropathic pain. An answerable question is whether SNT decreases AChE activity in spinal cord of frog Lithobates catesbeianus, a species that has been used as a model for studying SNT-induced neuropathic pain. Since each animal model has been created with a specific methodology, and the findings tend to vary widely with slight changes in the method used to induce pain, our study assessed AChE activity 3 and 10 days after complete SNT in lumbosacral spinal cord of adult male bullfrog Lithobates catesbeianus. Because there are time scale differences of motor endplate maturation in rat skeletal muscles, our study also measured the AChE activity in bullfrog tibial posticus (a postural muscle) and gastrocnemius (a typical skeletal muscle that is frequently used to study the motor system) muscles. AChE activity did not show significant changes 3 and 10 days following SNT in spinal cord. Also, no significant change occurred in AChE activity in tibial posticus and gastrocnemius muscles at day 3. However, a significant decrease was found at day 10, with reductions of 18% and 20% in tibial posticus and gastrocnemius, respectively. At present we cannot explain this change in AChE activity. While temporally different, the direction of the change was similar to that described for rats. This similarity indicates that bullfrog is a valid model for investigating AChE activity following SNT.<\/jats:p>","DOI":"10.1590\/1519-6984.03016","type":"journal-article","created":{"date-parts":[[2017,9,23]],"date-time":"2017-09-23T23:29:16Z","timestamp":1506209356000},"page":"217-223","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus"],"prefix":"10.1590","volume":"78","author":[{"given":"A.","family":"Kroth","sequence":"first","affiliation":[{"name":"Universidade do Oeste de Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Mackedanz","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Matt\u00e9","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. T. S.","family":"Wyse","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F. M.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. A.","family":"Partata","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2017,9,28]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.2174\/157488911795933901","article-title":"Analgesic and antineuropathic drugs acting through central cholinergic mechanisms","volume":"6","author":"BARTOLINI A.L.","year":"2011","journal-title":"Recent Patents on CNS Drug Discovery"},{"issue":"18","key":"ref2","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1097\/00001756-199412000-00059","article-title":"Synchronous, intersegmental responses in motoneurons to lumbar ventral root injury","volume":"5","author":"BURGESS 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