{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T04:40:30Z","timestamp":1720586430983},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract  Even today, an effective diagnostic test for aspergillosis in penguins is unknown, being the gold standard post-mortem examinations. The fungal antigen galactomannan (GM) has been used as a biomarker of disease in humans and is detected by the Platelia Aspergillus EIA (BioRad)\u00ae, a commercial kit based on the sandwich ELISA technique. It is standardized for use in neutropenic patients, however studies have demonstrated its usefulness also possible for birds. The aim of our study was to evaluate the effectiveness of Platelia Aspergillus EIA\u00ae test (BioRad-US) in the diagnosis of aspergillosis in Magellanic penguins, determining sensitivity, specificity, and positive and negative predictive values for different cut-off points. Were included in the study, blood serum samples (n = 29) Magellanic penguins in captivity that died by aspergillosis. Detection of GM was performed following manufacturer's instructions and the GM index was obtained by dividing the average value of OD of the duplicate of the clinical sample by duplicate OD of the average value of the cut-off sample provided by the kit. Through information database results were obtained for the presence of anti-Aspergillus fumigatus antibodies detected by agar gel immunodiffusion (AGID) for all serum samples. Results were analyzed using chi-square test and Kruskal-Wallis from SPSS 20.0, IBM\u00ae. ROC curve was obtained and from this, rates of sensitivity, specificity, positive and negative predictive values were also calculated based on four different cutoff points (0.5, 1.0, 1.5 and 2.0). The serum GM index did not differ between animals of the case and control group (pkw =0.097). In determining the ROC curve for serum GM detection the value of area under the curve was 0.635. From the values determined by the coordinate of the curve, four different cut points (0.5, 1.0, 1.5 and 2.0) were analyzed, resulting in sensitivity rates ranging from 86.2 to 34.5% % and specificity between 87% and 26.1%. By comparing the serum GM index in group case as the presence or absence of antibodies detected by AGID was found p=0.503. The detection of GM the Platelia Aspergillus EIA\u00ae test seems is not be useful for the diagnosis of aspergillosis in naturally infected penguins.<\/jats:p>","DOI":"10.1590\/1519-6984.171140","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T21:06:23Z","timestamp":1534626383000},"page":"169-173","source":"Crossref","is-referenced-by-count":6,"title":["Applicability of the Platelia EIA\u00ae Aspergillus test for the diagnosis of aspergilosis in penguins"],"prefix":"10.1590","volume":"79","author":[{"given":"A. L.","family":"Cabana","sequence":"first","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. O.","family":"Xavier","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Teles","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil; Universidade Federal do Rio Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. P.","family":"Silva-Filho","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C. A.","family":"Meireles","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2019,4]]},"reference":[{"issue":"13","key":"ref1","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1136\/vr.158.13.442","article-title":"Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons","volume":"158","author":"ARCA-RUIBAL B.","year":"2006","journal-title":"The Veterinary Record"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.vetmic.2008.07.018","article-title":"Comparison of the value of measurement of serum galactomannan and Aspergillus -specific antibodies in the diagnosis of canine sino-nasal aspergilosis","volume":"133","author":"BILLEN F.","year":"2009","journal-title":"Veterinary Microbiology"},{"key":"ref3","series-title":"Monitoramento sorol\u00f3gico para diagn\u00f3stico precoce da aspergilose em pinguins em cativeiro.","author":"CABANA A.L.","year":"2013"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1590\/S0100-736X2015000600015","article-title":"Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins","volume":"35","author":"CABANA \u00c2.L.","year":"2015","journal-title":"Pesquisa Veterin\u00e1ria Brasileira"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1647\/2007-041.1","article-title":"Galactomannan assay and plasma protein electrophoresis findings in psittacine birds with aspergillosis","volume":"23","author":"CRAY C.","year":"2009","journal-title":"Journal of Avian Medicine and Surgery"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1638\/2007-0138.1","article-title":"Application of galactomannan analysis and protein electrophoresis in the diagnosis of aspergillosis in avian species","volume":"40","author":"CRAY C.","year":"2009","journal-title":"Journal of Zoo and Wildlife Medicine"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/S1094-9194(03)00004-5","article-title":"Fungal diseases of birds of prey","volume":"6","author":"DEEM S.L.","year":"2003","journal-title":"The Veterinary Clinics of North America. 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