{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T06:13:18Z","timestamp":1792822398185,"version":"4.5.0"},"reference-count":21,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Pathogenic strains of Escherichia coli may invade the subcutaneous tissue of poultry and cause cellulitis, whilst the pathogen may also cause lesions in internal organs such as the liver. Current paper co-relates Escherichia coli and virulence genes characteristic of Avian Pathogenic Escherichia coli (APEC) in broilers\u00b4 cellulitis and liver lesions. One hundred carcasses were retrieved from the production chain in an avian abattoir in the state of Bahia, Brazil, between August 2013 and January 2014, due to detection of cellulitis lesions. Cellulitis and liver samples were retrieved aseptically to quantify E. coli by Petrifilm\u2122 count fast method (3M Company) (AOAC 998.8). Virulent genes iss and iutA were removed from E. coli isolates by Polymerase Chain Reaction (PCR). Escherichia coli was isolated from 82.0% of broilers removed from the production chain and the bacterium was concomitantly detected in cellulitis and liver lesions in 40.0% of broilers. E. coli counts ranged between 1.00 and 4.73 log CFU\/g in liver lesions and between 2.00 and 9.00 log UFC\/g in cellulitis lesions. Virulent genes iutA and iss were detected in 97.56% and 89.02% of E. coli isolates, respectively. Genotype analysis demonstrated the concomitant amplification of genes iutA and iss in 60.0% (n=40) of samples of cellulitis and liver lesions in which the simultaneous isolation of E. coli occurred. There was a positive and significant co-relationship (r=0.22; p&lt;0.05) between the variables occurrence of E. coli isolated from liver samples and the occurrence of E. coli isolated from cellulitis lesions. There were also positive and significant co-relationships between populations of E. coli from liver isolates and cellulitis lesions (r=0.46; p&lt;0.05) when E. coli isolated in the liver and in cellulitis lesions was detected. Since results showed a relationship between E. coli in cellulitis and liver lesions and possible systemic infection, the occurrence of cellulitis lesions as a criterion for total discarding of carcass may be suggested.<\/jats:p>","DOI":"10.1590\/1519-6984.230243","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T19:47:16Z","timestamp":1600717636000},"page":"714-718","source":"Crossref","is-referenced-by-count":5,"title":["Co-relationship between Escherichia coli in broiler cellulitis and liver lesions"],"prefix":"10.1590","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6443-134X","authenticated-orcid":false,"given":"R. M.","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Federal do Rec\u00f4ncavo da Bahia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9700-0354","authenticated-orcid":false,"given":"I. M. M","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rec\u00f4ncavo da Bahia,  Brasil; Centro de Ci\u00eancias Agr\u00e1rias Ambientais e Biol\u00f3gicas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2313-666X","authenticated-orcid":false,"given":"M. C.","family":"Jesus","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Agr\u00e1rias Ambientais e Biol\u00f3gicas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1127-3867","authenticated-orcid":false,"given":"M. D. B.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rec\u00f4ncavo da Bahia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0737-2144","authenticated-orcid":false,"given":"F. S.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rec\u00f4ncavo da Bahia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6026-1390","authenticated-orcid":false,"given":"J.","family":"Ev\u00eancio-Neto","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2021,9]]},"reference":[{"key":"ref1","series-title":"Avian histopathology.","author":"ABDUL-AZIZ T.","year":"2016","edition":"4th"},{"key":"ref2","series-title":"Relat\u00f3rio Anual 2018","year":"2018"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1007\/s11250-019-01823-3","article-title":"Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan","volume":"51","author":"AZAM M.","year":"2019","journal-title":"Tropical Animal Health and Production"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1590\/1519-6984.185930","article-title":"Virulence genes in Escherichia coli isolates from commercialized saltwater mussels Mytella guyanensis (Lamarck, 1819)","volume":"79","author":"BARBOSA C.A.","year":"2019","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"key":"ref5","series-title":"Portaria n\u00ba 210, de 10 de novembro de 1998. Regulamento T\u00e9cnico da Inspe\u00e7\u00e3o Tecnol\u00f3gica e Higi\u00eanico-Sanit\u00e1ria de Carne de Aves","year":"1998"},{"issue":"7","key":"ref6","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.1128\/IAI.71.7.4175-4177.2003","article-title":"Virulence factors and clonal relationships among Escherichia coli strains isolated from broiler chickens with cellulitis","volume":"71","author":"BRITO B.G.","year":"2003","journal-title":"Infection and Immunity"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00580-009-0837-4","article-title":"Virulence genotyping of Escherichia coli isolates from avian cellulitis in relation to phylogeny","volume":"19","author":"GHANBARPOUR R.","year":"2010","journal-title":"Comparative Clinical Pathology, Londres"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1023\/A:1005293400605","article-title":"Studies on Cellulitis and Other Disease Syndromes Caused by Escherichia coli in Broilers in Sri Lanka","volume":"32","author":"GOMIS S.M.","year":"2000","journal-title":"Tropical Animal Health and Production"},{"issue":"1","key":"ref9","first-page":"1","article-title":"Phenotypic and genotypic characterization of virulence factors of Escherichia coli isolated from broiler chickens with simultaneous occurrence of cellulitis and other colibacillosis lesions","volume":"65","author":"GOMIS S.M.","year":"2001","journal-title":"Canadian Journal of Veterinary Research"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1080\/03079450120078662","article-title":"The influence of Escherichia coli strains from different sources and the age of broiler chickens on the development of cellulitis","volume":"30","author":"JOHNSON L.C.","year":"2001","journal-title":"Avian Pathology"},{"issue":"8","key":"ref11","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1128\/AEM.02634-07","article-title":"Evolution of the iss gene in Escherichia coli.","volume":"74","author":"JOHNSON T.J.","year":"2008","journal-title":"Applied and Environmental Microbiology"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1100\/2012\/437342","article-title":"Prevalence of avian pathogenic Escherichia coli (APEC) clone harboring sfa gene in Brazil","volume":"7","author":"KN\u00d6BL T.","year":"2012","journal-title":"TheScientificWorldJournal"},{"issue":"8","key":"ref13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0105016","article-title":"Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil","volume":"9","author":"MALUTA R.P.","year":"2014","journal-title":"PLoS One"},{"issue":"2","key":"ref14","first-page":"93","article-title":"Principais causas de condena\u00e7\u00f5es no abate de frangos de corte de um abatedouro localizado na regi\u00e3o noroeste do Paran\u00e1 Brasil","volume":"15","author":"PASCHOAL E.C.","year":"2012","journal-title":"Arquivos de Ci\u00eancias Veterin\u00e1rias e Zoologia da UNIPAR"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1051\/vetres:2004057","article-title":"Characterizing the APEC pathotype","volume":"36","author":"RODRIGUEZ-SIEK K.E.","year":"2005","journal-title":"Veterinary Research"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1590\/S1519-99402012000300009","article-title":"Presen\u00e7a de Escherichia coli em f\u00edgados de frangos provenientes de matadouros av\u00edcolas","volume":"13","author":"SILVA I.M.M.","year":"2012","journal-title":"Revista Brasileira de Sa\u00fade e Produ\u00e7\u00e3o Animal"},{"key":"ref17","series-title":"Manual de m\u00e9todos de an\u00e1lise microbiol\u00f3gica de alimentos.","author":"SILVA N.","year":"2017","edition":"5th"},{"key":"ref18","series-title":"Diseases of poultry.","author":"SWAYNE D.E.","year":"2013","edition":"13th"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"174","DOI":"10.4322\/rbcv.2014.388","article-title":"Celulite em frangos de corte abatidos sob inspe\u00e7\u00e3o sanit\u00e1ria: aspectos anatomopatol\u00f3gicos associados ao isolamento de Escherichia coli.","volume":"13","author":"VIEIRA T.B.","year":"2006","journal-title":"Revista Brasileira de Ci\u00eancia Veterin\u00e1ria"},{"issue":"2","key":"ref20","first-page":"144","article-title":"Potencial patog\u00eanico e car\u00e1ter s\u00e9ptico de Escherichia coli pela identifica\u00e7\u00e3o dos fatores de virul\u00eancia iss e felA em celulite e mi\u00fados de frangos sob Inspe\u00e7\u00e3o Sanit\u00e1ria","volume":"36","author":"VIEIRA T.B.","year":"2014","journal-title":"Revista Brasileira de Medicina Veterin\u00e1ria"},{"issue":"3","key":"ref21","first-page":"247","article-title":"An\u00e1lise molecular de Escherichia coli isoladas de celulite e mi\u00fados (f\u00edgado e cora\u00e7\u00e3o) de frangos sob inspe\u00e7\u00e3o sanit\u00e1ria","volume":"35","author":"VIEIRA T.B.","year":"2013","journal-title":"Revista Brasileira de Medicina Veterin\u00e1ria"}],"container-title":["Brazilian Journal of Biology"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/bjb\/v81n3\/1519-6984-bjb-1519-6984230243.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T17:12:13Z","timestamp":1614186733000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842021000300714&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":21,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["S1519-69842021000300714"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.230243","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]}}}