{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:47:24Z","timestamp":1765295244516,"version":"3.41.2"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Allium sativum L. is an herb of the Alliaceae family with a specific taste and aroma and medicinal and nutraceutical properties that are widely marketed in several countries. Brazil is one of the largest importers of garlic in the world, despite of its production is restricted and limited to internal consumption. Thus, explore the genetic diversity of commercial garlic conserved at germplasm banks is essential to generate additional genetic information about its economically important crop. A suitable tool for this purpose is the cytogenetic characterisation of these accessions. This study aimed to characterise the cytogenetic diversity among seven accessions of garlic from a Germplasm Bank in Brazil. The karyotypes were obtained by conventional staining and with chromomycin A3 (CMA) and 4,6-diamidino-2-phenylindole (DAPI) fluorochromes. All accessions analysed showed chromosome number 2n = 16, karyotype formula 6M+2SM, symmetrical karyotypes, reticulate interphase nuclei, and chromosomes with uniform chromatin condensation from prophase to metaphase. The fluorochromes staining showed differences in the amount and distribution of heterochromatin along the chromosomes and between accessions studied. Based on the distribution pattern of these small polymorphisms, it was possible to separate the seven accessions into three groups. It was also possible to differentiate some of the accessions individually. One of the results obtained showed a heteromorphic distension of the nucleolar organiser region observed on the chromosome pairs 6 or 7 with peculiar characteristics. It was suggested for example, that the heteromorphic block of heterochromatin (CMA+++\/DAPI-) on chromosome 6 of the \u201cBranco Mineiro Piau\u00ed\u201d accession can be used as a marker to identify this genotype or may be associated with some character of economic interest.<\/jats:p>","DOI":"10.1590\/1519-6984.243514","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T22:32:44Z","timestamp":1623709964000},"source":"Crossref","is-referenced-by-count":4,"title":["Variations in heterochromatin content reveal important polymorphisms for studies of genetic improvement in garlic (Allium sativum L.)"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6509-2327","authenticated-orcid":false,"given":"P. A. A.","family":"Bacelar","sequence":"first","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7884-7058","authenticated-orcid":false,"given":"L. L.","family":"Feitoza","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil; Universidade Federal Rural de Pernambuco,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2953-7330","authenticated-orcid":false,"given":"S. E. S.","family":"Valente","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7700-6959","authenticated-orcid":false,"given":"R. L. F.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4645-9055","authenticated-orcid":false,"given":"L. V.","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5431-6818","authenticated-orcid":false,"given":"P. M.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7739-5061","authenticated-orcid":false,"given":"V. B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9546-5403","authenticated-orcid":false,"given":"A. C. A.","family":"Lopes","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3515-2111","authenticated-orcid":false,"given":"R.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2598-2621","authenticated-orcid":false,"given":"A. P.","family":"Peron","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil; Universidade Tecnol\u00f3gica Federal do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1046\/j.1095-8339.2002.00047.x","article-title":"Chromosomal organization of ribosomal genes and NOR-associated heterochromatin, and NOR activity in some populations of Allium commutatum Guss. (Alliaceae)","volume":"139","author":"BESENDORFER V.","year":"2002","journal-title":"Botanical Journal of the Linnean Society"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","DOI":"10.1590\/1678-4685-gmb-2018-0171","article-title":"Heterochromatin and numeric chromosome evolution in Bignoniaceae, with emphasis on the Neotropical clade Tabebuia alliance","volume":"43","author":"CORDEIRO J.M.","year":"2020","journal-title":"Genetics and Molecular Biology"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1080\/00087114.1983.10797661","article-title":"C-banding and sister chromatid exchanges in three species of the genus Allium (A. cepa, A. ascalonicum and A. sativum)","volume":"36","author":"CORTES F.","year":"1983","journal-title":"Caryologia"},{"issue":"3","key":"ref4","first-page":"271","article-title":"GENES: a software package for analysis in experimental statistics and quantitative genetics","volume":"35","author":"CRUZ C.D.","year":"2013","journal-title":"Acta Scientiarum. 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R.","year":"1986","journal-title":"Taxon"}],"container-title":["Brazilian Journal of Biology"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/bjb\/v83\/1519-6984-bjb-83-e243514.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T22:33:00Z","timestamp":1623709980000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842023000100104&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":36,"alternative-id":["S1519-69842023000100104"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.243514","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e243514"}}