{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:03:50Z","timestamp":1753887830410,"version":"3.41.2"},"reference-count":55,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Nucleotide excision repair (NER) acts repairing damages in DNA, such as lesions caused by cisplatin. Xeroderma Pigmentosum complementation group C (XPC) protein is involved in recognition of global genome DNA damages during NER (GG-NER) and it has been studied in different organisms due to its importance in other cellular processes. In this work, we studied NER proteins in Trypanosoma cruzi and Trypanosoma evansi, parasites of humans and animals respectively. We performed three-dimensional models of XPC proteins from T. cruzi and T. evansi and observed few structural differences between these proteins. In our tests, insertion of XPC gene from T. evansi (TevXPC) in T. cruzi resulted in slower cell growth under normal conditions. After cisplatin treatment, T. cruzi overexpressing its own XPC gene (TcXPC) was able to recover cell division rates faster than T. cruzi expressing TevXPC gene. Based on these tests, it is suggested that TevXPC (being an exogenous protein in T. cruzi) interferes negatively in cellular processes where TcXPC (the endogenous protein) is involved. This probably occurred due interaction of TevXPC with some endogenous molecules or proteins from T.cruzi but incapacity of interaction with others. This reinforces the importance of correctly XPC functioning within the cell.<\/jats:p>","DOI":"10.1590\/1519-6984.243910","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:44:06Z","timestamp":1624927446000},"source":"Crossref","is-referenced-by-count":0,"title":["Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9787-0456","authenticated-orcid":false,"given":"K. 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M.","family":"Repol\u00eas","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-9773","authenticated-orcid":false,"given":"B. C.","family":"Resende","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4246-8045","authenticated-orcid":false,"given":"R. S.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Instituto Federal de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5926-0286","authenticated-orcid":false,"given":"L. C.","family":"Miletti","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8724-3165","authenticated-orcid":false,"given":"C. R.","family":"Machado","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1715-2556","authenticated-orcid":false,"given":"C. I. G.","family":"Vogel","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"10","key":"ref1","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1093\/hmg\/10.16.1627","article-title":"Peptide-N-glycanases and DNA repair proteins, Xp-C\/Rad4, are, respectively, active and inactivated enzymes sharing a common transglutaminase fold","volume":"1","author":"ANANTHARAMAN V.","year":"2001","journal-title":"Human Molecular Genetics"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1093\/nar\/gkp851","article-title":"TriTrypDB: a functional genomic resource for the Trypanosomatidae","volume":"38","author":"ASLETT M.","year":"2010","journal-title":"Nucleic Acids Research"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1111\/mmi.12435","article-title":"Trypanosoma brucei harbours a divergent XPB helicase paralogue that is specialized in nucleotide excision repair and conserved among kinetoplastid organisms","volume":"90","author":"BADJATIA N.","year":"2013","journal-title":"Molecular Microbiology"},{"key":"ref4","doi-asserted-by":"crossref","DOI":"10.1155\/2010\/389153","article-title":"Immunobiology of African trypanosomes: need of alternative interventions","volume":"2010","author":"BARAL T.N.","year":"2010","journal-title":"Journal of Biomedicine & Biotechnology"},{"issue":"6","key":"ref5","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1083\/jcb.201107050","article-title":"Recognition of DNA damage by XPC coincides with disruption of the XPC-RAD23 complex","volume":"196","author":"BERGINK S.","year":"2012","journal-title":"The Journal of Cell Biology"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The Protein Data Bank","volume":"28","author":"BERMAN H.M.","year":"2000","journal-title":"Nucleic Acids Research"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1056\/NEJMra1410150","article-title":"Chagas\u2019 Disease","volume":"30","author":"BERN C.","year":"2015","journal-title":"The New England Journal of Medicine"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1590\/1519-6984.228876","article-title":"Validation of a documentary on Chagas disease by a population living in an endemic area","volume":"81","author":"BIANCHI T.F.","year":"2021","journal-title":"Brazilian Journal of Biology = Revista brasleira de Biologia"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1093\/nar\/gkaa977","article-title":"The InterPro protein families and domains database: 20 years on","volume":"49","author":"BLUM M.","year":"2021","journal-title":"Nucleic Acids Research"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nrm3350","article-title":"TFIIH: when transcription met DNA repair","volume":"13","author":"COMPE E.","year":"2012","journal-title":"Nature Reviews. 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