{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T20:47:14Z","timestamp":1782420434206,"version":"3.54.5"},"reference-count":43,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Tiliroside is a glycosidic flavonoid present in many plants species including Helicteres velutina K. Schum (Malvaceae sensu lato), commonly known in Brazil as \u201cpit\u00f3\u201d. This molecule has been shown to have many biological activities, however no study has been carried out to investigate the toxicity of this substance. The present work aimed to evaluate the possible cellular toxicity in silico, in vitro and ex-vivo of the kaempferol-3-O-\u03b2-D-(6\u201d-E-p-coumaroyl) glucopyranoside (tiliroside), through chemical structure analysis, toxicity assessment and predictive bioactive properties, using human samples for in vitro and ex-vivo tests. The in silico analysis suggests that tiliroside exhibited great absorption index when penetrating biological membranes. In addition, it also displayed considerable potential for cellular protection against free radicals, and anticarcinogenic, antioxidant, antineoplastic, anti-inflammatory, anti-hemorrhagic and antithrombotic activities. The assessment of the hemolytic and genotoxic effects of tiliroside showed low hemolysis rates in red blood cells and absence of cellular toxicity in the oral mucosa cells. The data obtained indicate that this molecule could be a promising therapeutic approach as a possible new drug with biotechnological potential.<\/jats:p>","DOI":"10.1590\/1519-6984.244127","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T12:13:41Z","timestamp":1624277621000},"source":"Crossref","is-referenced-by-count":6,"title":["Analysis of the toxicological and pharmacokinetic profile of Kaempferol-3-O-\u03b2-D-(6\u201d-E-p-coumaryl) glucopyranoside - Tiliroside: in silico, in vitro and ex vivo assay"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3430-477X","authenticated-orcid":false,"given":"A. P.","family":"Sousa","sequence":"first","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6120-761X","authenticated-orcid":false,"given":"D. A.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8156-3443","authenticated-orcid":false,"given":"M. D. L.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8884-7331","authenticated-orcid":false,"given":"L. V.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9936-7130","authenticated-orcid":false,"given":"M. F. V.","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil; Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7420-2014","authenticated-orcid":false,"given":"H. L. F.","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7466-9933","authenticated-orcid":false,"given":"A. A.","family":"Oliveira Filho","sequence":"additional","affiliation":[{"name":"Universidade Federal de Campina Grande,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8545-7928","authenticated-orcid":false,"given":"R. C. S.","family":"S\u00e1","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"key":"ref1","series-title":"Toxicity of tiliroside","year":"2012"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3389\/fphar.2016.00059","article-title":"Tinospora crispa (L.) Hook. f. & Thomson: a review of its ethnobotanical, phytochemical, and pharmacological aspects","volume":"7","author":"AHMAD W.","year":"2016","journal-title":"Frontiers in Pharmacology"},{"key":"ref3","first-page":"514","article-title":"Usos medicinais, fitoqu\u00edmica e farmacologia da fam\u00edlia Sterculiaceae: uma revis\u00e3o","volume":"92","author":"AL MUQARRABUN L.M.R.","year":"2015","journal-title":"Revista Europ\u00e9ia de Qu\u00edmica Medicinal"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1590\/1984-0462\/;2017;35;3;00010","article-title":"Genotoxicidade e citotoxicidade dos raios x em crian\u00e7as submetidas \u00e0 radiografia panor\u00e2mica","volume":"35","author":"ANTONIO E.L.","year":"2017","journal-title":"Revista Paulista de Pediatria : Orgao Oficial da Sociedade de Pediatria de Sao Paulo"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.jep.2014.09.013","article-title":"Toxicological evaluation of hydroethanolic extract of Helicteres sacarolha A. 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