{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:45:23Z","timestamp":1754484323219,"version":"3.41.2"},"reference-count":65,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Excessive intake of non-steroidal anti-inflammatory drugs such as, diclofenac sodium (DS) may lead to toxicity in the rats. In this work, we aimed to examine the protective impact of lentil extract (LE) and folic acid (FA) on the hematological markers, the kidney tissue oxidative stress and the renal function against diclofenac sodium (DS) in male albino rats. The rats (120-150 g) were divided into four equal groups randomly, the first group kept as the untreated control. The second group was administrated with DS (11.6 mg\/kg b.wt. orally once\/day). The third group was received DS+FA (11.6 mg\/kg b.wt.+76.9 microgram\/kg b.wt.) orally once\/day. The fourth group was treated with DS+LE (11.6 mg\/kg b.wt.+500 mg\/kg b.wt.) orally once\/day. After four weeks, the results revealed that DS produced a significant decrease in the values of red blood cells (RBCs), hemoglobin concentration (Hb), hematocrit (HCT) and white blood cells (WBCs). On the other hand, there was a significant increase in the platelets count. Also, DS induced a renal deterioration; this was evidenced by the significant increase in the serum levels of urea, creatinine, uric acid, Na, Ca, Mg as well as the nitric oxide (NO) level in the kidney tissue. Also, there were a significant reduction in the serum levels of potassium (K) and reduced glutathione (GSH) in the kidney homogenates. Moreover, the findings in the rats treated by DS+LE or DS+FA showed a potential protection on the hematological markers, oxidative stress in the kidney tissue and the renal function disturbed by DS. LE and FA could play a potent role for the prevention the adverse hematological, the kidney tissue oxidative stress and the renal dysfunction caused by DS via their anti-oxidative and bioactive phytochemicals.<\/jats:p>","DOI":"10.1590\/1519-6984.247360","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T12:19:18Z","timestamp":1637324358000},"source":"Crossref","is-referenced-by-count":5,"title":["Hematological and renoprotective effects of folic acid and lentil extract in diclofenac sodium exposed rats"],"prefix":"10.1590","volume":"83","author":[{"given":"Omnia N.","family":"Abdel-Rahman","sequence":"first","affiliation":[{"name":"Ain Shams University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8331-4472","authenticated-orcid":false,"given":"Enas S.","family":"Abdel-Baky","sequence":"additional","affiliation":[{"name":"Ain Shams University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.3390\/ijms19020344","article-title":"Lycopene attenuates tulathromycin and diclofenac sodium-induced cardiotoxicity in mice","volume":"19","author":"ABDEL-DAIM M.M.","year":"2018","journal-title":"International Journal of Molecular Sciences"},{"issue":"1","key":"ref2","first-page":"14","article-title":"Ameliorative effects of Kolaviron, a biflavonoid fraction from Garcinia Kola seed, on hepatorenal toxicity of anti-tuberculosis drugs in wistar rats","volume":"41","author":"ADARAMOYE O.A.","year":"2016","journal-title":"The Tokai Journal of Experimental and Clinical Medicine"},{"issue":"2","key":"ref3","first-page":"1","article-title":"Evaluation of Biochemical effects of dioclofenac sodium in goats","volume":"22","author":"AHMAD I.","year":"2012","journal-title":"Journal 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of Biological and Chemical Sciences"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1517\/14740338.2013.780595","article-title":"Safety of celecoxib compared with placebo and non-selective NSAIDs: cumulative meta-analysis of 89 randomized controlled trials","volume":"12","author":"ESSEX M.N.","year":"2013","journal-title":"Expert Opinion on Drug Safety"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s12349-012-0109-8","article-title":"Role of lentils (Lens culinaris L.) in human health and nutrition: a review","volume":"6","author":"FARIS M.A.E.","year":"2013","journal-title":"Mediterranean Journal of Nutrition and Metabolism"},{"year":"1982","series-title":"Selected methods for the small clinical chemistry laboratory","author":"FAULKER W.R.","key":"ref20"},{"year":"2011","series-title":"Economic and Social Department: The Statistical Division","key":"ref21"},{"key":"ref22","first-page":"150","article-title":"Traditional food plants","year":"1998","journal-title":"Food and Nutrition Paper"},{"issue":"11","key":"ref23","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.3390\/ijms18112390","article-title":"Polyphenol-rich lentils and their health promoting effects","volume":"18","author":"GANESAN K.","year":"2017","journal-title":"International Journal of Molecular Sciences"},{"year":"1992","series-title":"Primer of biostatistics.","author":"GLANTZ A.S.","key":"ref24"},{"issue":"4","key":"ref25","first-page":"170","article-title":"Markers of renal function","volume":"2","author":"GOWDA S.","year":"2010","journal-title":"North American Journal of Medical Sciences"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0891-5849(01)00560-3","article-title":"Diclofenac induced in vivo nephrotoxicity may involve oxidative stress-mediated massive genomic DNA fragmentation and 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free radical hypothesis: reactive oxygen species\u2014cause or consequence of tissue injury","volume":"24","author":"JUR\u00c1NEK I.","year":"2005","journal-title":"General Physiology and Biophysics"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"116","DOI":"10.5049\/EBP.2007.5.2.116","article-title":"Electrolyte and acid-base disturbances associated with nonsteroidal anti-inflammatory drugs","volume":"5","author":"KIM S.","year":"2007","journal-title":"Electrolyte & Blood Pressure"},{"issue":"2-3","key":"ref32","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2298\/AVB0703241K","article-title":"Effect of flunixin meglumin on the antioxidant status in endotoxemia","volume":"57","author":"KONYALIOGLU S.","year":"2007","journal-title":"Acta Veterinaria"},{"issue":"10","key":"ref33","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1038\/nrneurol.2009.138","article-title":"Neurological complications of chronic kidney 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ethanol","volume":"5","author":"LEE S.","year":"2011","journal-title":"Nutrition Research and Practice"},{"issue":"1-2","key":"ref37","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1006\/mgme.2000.3027","article-title":"Folic acid: nutritional biochemistry, molecular biology, and role in disease processes","volume":"71","author":"LUCOCK M.","year":"2000","journal-title":"Molecular Genetics and Metabolism"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/bja\/aeg034","article-title":"Oxidative stress and gene expression in sepsis","volume":"90","author":"MACDONALD J.","year":"2003","journal-title":"British Journal of Anaesthesia"},{"issue":"2","key":"ref39","first-page":"100","article-title":"Prunus armeniaca leaves extract protects against isoniazid and rifampicin induced nephrotoxicity through modulation of oxidative stress and inflammation","volume":"2","author":"MAHMOUD A.M.","year":"2015","journal-title":"International Journal of Food and Nutrition Science"},{"issue":"7","key":"ref40","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1080\/0886022X.2016.1184940","article-title":"Amelioration of anti-tuberculosis drug induced oxidative stress in kidneys by Spirulina fusiformis in a rat model","volume":"38","author":"MARTIN S.J.","year":"2016","journal-title":"Renal Failure"},{"issue":"11","key":"ref41","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.3111\/13696990903246911","article-title":"Diclofenac plus B vitamins versus diclofenac monotherapy in lumbago: the DOLOR study","volume":"25","author":"MIBIELLI M.A.","year":"2009","journal-title":"Current Medical Research and Opinion"},{"issue":"14","key":"ref42","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1161\/CIRCULATIONAHA.107.725481","article-title":"High-dose folic acid pretreatment blunts cardiac dysfunction during ischemia coupled to maintenance of high-energy phosphates and reduces postreperfusion injury","volume":"117","author":"MOENS A.L.","year":"2008","journal-title":"Circulation"},{"key":"ref43","first-page":"414","article-title":"The determination of nitrite in water","volume":"86","author":"MONTGOMERY H.A.C.","year":"1961","journal-title":"Analyst"},{"key":"ref44","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/1342549","article-title":"The effects of folic acid administration on cardiac oxidative stress and cardiovascular biomarkers in diabetic rats","volume":"2019","author":"MUTAVDZIN S.","year":"2019","journal-title":"Oxidative Medicine and Cellular Longevity"},{"issue":"3","key":"ref45","first-page":"158","article-title":"Complementary and alternative medicine use in diabetes mellitus","volume":"29","author":"OGBERA A.O.","year":"2010","journal-title":"West African Journal of Medicine"},{"issue":"5","key":"ref46","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.4314\/ijbcs.v10i5.23","article-title":"Haematological and biochemical studies on the effect of diclofenac sodium on Wistar Rattus norvegicus","volume":"10","author":"ORINYA O.A.","year":"2016","journal-title":"International Journal of Biological and Chemical Sciences"},{"key":"ref47","series-title":"Evaluation of drug activities: pharmacometrics","first-page":"135","volume-title":"Toxicity tests.","volume":"I","author":"PAGET G.E.","year":"1964"},{"key":"ref48","article-title":"Effect of diclofenac with B vitamins on the treatment of acute pain originated by lower-limb fracture and surgery","volume":"2012","author":"PONCE-MONTER H.A.","year":"2012","journal-title":"Pain Research and Treatment"},{"key":"ref49","series-title":"Diagnostic hematology.","first-page":"128","volume-title":"Routine testing in hematology.","author":"RODAK L.C.","year":"1995"},{"issue":"3","key":"ref50","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1039\/C7TX00309A","article-title":"Beneficial effect of folic acid on kidney and testes of adult albino rats after exposure to methomyl","volume":"7","author":"SAKR S.","year":"2018","journal-title":"Toxicology Research"},{"issue":"3","key":"ref51","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/intqhc\/mzg034","article-title":"Assessing residents\u2019 prescribing behavior in renal impairment","volume":"15","author":"SALOMON L.","year":"2003","journal-title":"International Journal for Quality in Health Care"},{"issue":"4","key":"ref52","doi-asserted-by":"crossref","first-page":"290","DOI":"10.9734\/EJMP\/2012\/1517","article-title":"Haematological and hepatotoxic potential of onion (Allium cepa) and garlic (Allium sativum) extracts in rats","volume":"2","author":"SAMSON E.S.","year":"2012","journal-title":"European Journal of Medicinal Plants"},{"issue":"4","key":"ref53","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.26717\/BJSTR.2018.03.000940","article-title":"Folate content in legumes","volume":"3","author":"SINGH J.","year":"2018","journal-title":"Biomedical Journal of Scientific & Technical Research"},{"issue":"8","key":"ref54","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1038\/sj.ijo.0803265","article-title":"Effect of short-term folic acid supplementation on insulin sensitivity and inflammatory markers in overweight subjects","volume":"30","author":"SOLINI A.","year":"2006","journal-title":"International Journal of Obesity"},{"issue":"6","key":"ref55","first-page":"795","article-title":"The protective effect of soybean, sesame, lentils, pumpkin seeds and molasses on iron deficiency anemia in rats","volume":"23","author":"SOLTAN S.S.A.","year":"2013","journal-title":"World Applied Sciences Journal"},{"issue":"6","key":"ref56","first-page":"253","article-title":"Determination of calcium, sodium, potassium and magnesium in human blood sera by atomic absorption","volume":"44","author":"SZ\u00cdV\u00d3S K.","year":"1974","journal-title":"Acta Pharmaceutica Hungarica"},{"issue":"2-3","key":"ref57","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/j.jhazmat.2008.04.120","article-title":"Determination of elemental composition of molasses and its suitability as carbon source for growth of sulphate - reducing bacteria","volume":"161","author":"TECLU D.","year":"2009","journal-title":"Journal of Hazardous Materials"},{"key":"ref58","series-title":"Phenomics in crop plants: trends, options and limitations.","first-page":"223","volume-title":"Phenotyping nutritional and antinutritional traits.","author":"THAVARAJAH D.","year":"2015"},{"key":"ref59","series-title":"Fundamentals of clinical chemistry","first-page":"874","author":"TIETZ N.W.","year":"1987","edition":"3rd"},{"year":"2010","series-title":"Database for the Oxygen Radical Absorbance Capacity (ORAC) of selected foods","key":"ref60"},{"year":"2011","series-title":"USDA National Nutrient Database for Standard Reference","key":"ref61"},{"key":"ref62","series-title":"Textbook of biochemistry for medical students.","author":"VASUDEVAN D.M.","year":"2007","edition":"5th"},{"issue":"1","key":"ref63","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1161\/hq0102.102190","article-title":"Folates and cardiovascular disease","volume":"22","author":"VERHAAR M.C.","year":"2002","journal-title":"Arteriosclerosis Thrombosis and Vascular Biology"},{"issue":"6","key":"ref64","first-page":"363","article-title":"Protective effect of L carnitine against diclofenac sodium toxicity in mice","volume":"159","author":"YAPAR K.","year":"2008","journal-title":"Revue de Medecine Veterinaire"},{"key":"ref65","series-title":"Effects of disease on clinical lab.tests","author":"YOUNG D.S.","year":"2001","edition":"4th"}],"container-title":["Brazilian Journal of 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