{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:08:06Z","timestamp":1785546486525,"version":"3.56.0"},"reference-count":32,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Staphylococcus aureus is an important foodborne pathogen associated to food intoxication and other multiple infections in human being. Its presence in salted food is a serious issue due to its salt tolerance potential. A study was conducted to analyze the presence of enterotoxins producing drug resistance S. aureus in salted sea fish from Gwadar. Freshly persevered samples (n=50) of salted fish were subjected to analyze the presence of S. aureus using 16S rRNA and Nuc genes primers. The isolates were then evaluated for drug resistance and enterotoxins producing potential using specific primers for MecA (methicillin resistance gene), (SEA) staphylococcal enterotoxin A and (SEB) staphylococcal enterotoxin B genes. Total 13\/50 (26%) of the samples were found positive for the presence of S. aureus, preliminary confirmed with biochemical profiling and finally with the help of target genes presence. The isolates were found showing 100% resistant to methicillin, which were molecularly confirmed by the presence of MecA gene present in genome. The isolates 5\/13 (38%) were positive for SEA and 3\/13 (23%) for SEB genes, whereas 2\/13 (15%) were confirmed having both SEA and SEB genes in its genome. It was also confirmed that all the isolates were capable to form biofilm over the glass surfaces. It was concluded that the study confirmed the presence of enterotoxigenic methicillin resistance Staphylococcus aurous (MRSA) in salted fish product, that poses gross food safety concern. Preventive and control measures are necessary to handle this serious food safety concern.<\/jats:p>","DOI":"10.1590\/1519-6984.247701","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T12:39:22Z","timestamp":1630067962000},"source":"Crossref","is-referenced-by-count":9,"title":["Enterotoxigenic methicillin resistant Staphylococcus aureus contamination in salted fish from Gwadar Balochistan"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4408-9943","authenticated-orcid":false,"given":"N.","family":"Rashid","sequence":"first","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7600-5391","authenticated-orcid":false,"given":"M.","family":"Shafee","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6197-4058","authenticated-orcid":false,"given":"S.","family":"Iqbal","sequence":"additional","affiliation":[{"name":"University of Information Technology Engineering and Management Sciences,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9647-2165","authenticated-orcid":false,"given":"A.","family":"Samad","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5117-0059","authenticated-orcid":false,"given":"S. A.","family":"Khan","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9841-2569","authenticated-orcid":false,"given":"M. S.","family":"Hasni","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-8140","authenticated-orcid":false,"given":"Z. U.","family":"Rehman","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2193-4542","authenticated-orcid":false,"given":"S.","family":"Ullah","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8006-0622","authenticated-orcid":false,"given":"F. U.","family":"Rehman","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5982-6558","authenticated-orcid":false,"given":"G. I.","family":"Khan","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4529-5362","authenticated-orcid":false,"given":"S.","family":"Ahmad","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0376-1425","authenticated-orcid":false,"given":"A.","family":"Akbar","sequence":"additional","affiliation":[{"name":"University of Balochistan,  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"1","key":"ref1","first-page":"5","article-title":"Food safety concerns and food-borne pathogens, Salmonella, Escherichia coli and Campylobacter","volume":"1","author":"AKBAR A.","year":"2011","journal-title":"FUUAST Journal of Biology"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s13213-013-0641-x","article-title":"Occurrence of Staphylococcus aureus and evaluation of anti-staphylococcal activity of Lactococcus lactis subsp. lactis in ready-to-eat poultry meat","volume":"64","author":"AKBAR A.","year":"2014","journal-title":"Annals of Microbiology"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.1007\/s13197-014-1354-2","article-title":"Isolation of Salmonella from ready-to-eat poultry meat and evaluation of its survival at low temperature, microwaving and simulated gastric fluids","volume":"52","author":"AKBAR A.","year":"2015","journal-title":"Journal of Food Science and Technology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.bcab.2018.11.005","article-title":"Synthesis and antimicrobial activity of zinc oxide nanoparticles against foodborne pathogens Salmonella typhimurium and Staphylococcus aureus","volume":"17","author":"AKBAR A.","year":"2019","journal-title":"Biocatalysis and Agricultural Biotechnology"},{"issue":"3","key":"ref5","first-page":"941","article-title":"Presence of Escherichia coli in poultry meat: a potential food safety threat","volume":"21","author":"AKBAR A.","year":"2014","journal-title":"International Food Research Journal"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"34216","DOI":"10.1038\/srep34216","article-title":"Characterization of toxin genes and antimicrobial susceptibility of Staphylococcus aureus isolates in fishery products in Iran","volume":"6","author":"ARFATAHERY N.","year":"2016","journal-title":"Scientific Reports"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1515\/pjvs-2017-0012","article-title":"Molecular characterization and detection of enterotoxins, methicillin resistance genes and antimicrobial resistance of Staphylococcus aureus from fish and ground beef","volume":"20","author":"ARSLAN S.","year":"2017","journal-title":"Polish Journal of Veterinary Sciences"},{"issue":"5","key":"ref8","first-page":"427","article-title":"Prevalence of Staphylococcus aureus in fish samples of local domestic fish market","volume":"4","author":"BUJJAMMA P.","year":"2015","journal-title":"International Journal of Microbiology and Applied Sciences"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"22","DOI":"10.9734\/mrji\/2020\/v30i330202","article-title":"Detection of Methicillin-Resistant Staphylococcus aureus in Ready-to-Eat Shellfish (Corbiculid heterodont) in Bayelsa State, Nigeria","volume":"30","author":"EGEGE S.R.","year":"2020","journal-title":"Microbiology Research Journal International"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1590\/1519-6984.170727","article-title":"Identification and antimicrobial suceptibility profile of bacteria causing bovine mastitis from dairy farms in 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and nutrients in the average Polish diet","volume":"10","author":"LASKOWSKI W.","year":"2018","journal-title":"Nutrients"},{"issue":"10","key":"ref17","doi-asserted-by":"crossref","first-page":"490","DOI":"10.3390\/foods8100490","article-title":"Review on natural preservatives for extending fish shelf life","volume":"8","author":"MEI J.","year":"2019","journal-title":"Foods"},{"issue":"2","key":"ref18","first-page":"83","article-title":"Prevalence of staphylococci in commercially processed food products in Karachi-Pakistan","volume":"7","author":"MIRANI Z.A.","year":"2017","journal-title":"Journal of Microbiology and Infectious Diseases"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3923\/mj.2014.51.56","article-title":"PCR Deyection of Staphylococcal Enterotoxin A and B Genes in Staphylococcus aureus Isolated from Salted Fermented Fish","volume":"4","author":"MOHAMMED T.A.","year":"2014","journal-title":"Microbiology 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Microbiology"},{"issue":"5","key":"ref26","doi-asserted-by":"crossref","first-page":"844","DOI":"10.4315\/JFP-19-546","article-title":"Survival of Methicillin-Resistant Staphylococcus aureus in fish and Shrimp under different storage conditions","volume":"83","author":"SAKLANI P.","year":"2020","journal-title":"Journal of Food Protection"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","DOI":"10.1111\/jfs.12600","article-title":"Prevalence, antimicrobial susceptibility, and virulence of Campylobacter jejuni isolated from chicken meat","volume":"39","author":"SAMAD A.","year":"2019","journal-title":"Journal of Food Safety"},{"key":"ref28","series-title":"Antimicrobial resistance in agriculture: perspective, policy and mitigation","first-page":"195","volume-title":"Staphylococcus","author":"SAMANTA I.","year":"2020"},{"issue":"2","key":"ref29","first-page":"424","article-title":"Isolation of some human pathogens from fresh and smoked shad","volume":"11","author":"TAVAKOLI 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