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Biol."],"abstract":"<jats:p>Abstract By applying the in-silico method, resveratrol was docked on those proteins which are responsible for bone loss. The Molecular docking data between the resveratrol and Receptor activator of nuclear factor-kappa-\u0392 ligand [RANKL] receptors proved that resveratrol binds tightly to the receptors, showed the highest binding affinities of \u22126.9, \u22127.6, \u22127.1, \u22126.9, \u22126.7, and \u22127.1 kcal\/mol. According to in-vitro data, Resveratrol reduced the osteoclasts after treating Marrow-Derived Macrophages [BMM] with Macrophage colony-stimulating factor [MCSF] 20ng \/ ml and RANKL 50ng \/ ml, with different concentrations of resveratrol (2.5, 10 \u03bcg \/ ml) For 7 days, the cells were treated with MCSF (20 ng \/ ml) and RANKL (40 ng \/ ml) together with concentrated trimethyl ether and resveratrol (2.5, 10 \u03bcg \/ ml) within 12 hours. Which, not affect cell survival. After fixing osteoclast cells with formaldehyde fixative on glass coverslip followed by incubation with 0.1% Triton X-100 in PBS for 5 min and after that stain with rhodamine phalloidin staining for actin and Hoechst for nuclei. Fluorescence microscopy was performed to see the distribution of filaments actin [F.actin]. Finally, resveratrol reduced the actin ring formation. Resveratrol is the best bioactive compound for drug preparation against bone loss.<\/jats:p>","DOI":"10.1590\/1519-6984.248024","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T13:11:35Z","timestamp":1640005895000},"source":"Crossref","is-referenced-by-count":7,"title":["Study of resveratrol against bone loss by using in-silico and in-vitro methods"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2675-2388","authenticated-orcid":false,"given":"S. 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