{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T02:54:10Z","timestamp":1761706450295,"version":"3.41.2"},"reference-count":39,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Being vector of West Nile Virus and falariasis the control of Culex quinquefasciatus is likely to be essential. Synthetic insecticide treatment is looking most effective for vectors mosquito control. However, these products are toxic to the environment and non-target organisms. Consequently, ecofriendly control of vectors mosquito is needed. In this regard botanical insecticide is looking more fruitful. Therefore, the present research aimed to investigate the effectiveness of methanolic extract and various fractions, including, n-hexane, ethyl-acetate, chloroform, and aqueous fraction, obtained from methanolic extract of Ailanthus altissima, Artemisia scoparia, and Justicia adhatoda using separating funnel against larval, pupal, and adult stages of Culex quinquefasciatus. The larvae and pupae of Culex quinquefasciatus were exposed to various concentrations (31.25-1000 ppm) of methanolic extract and its fractions for 24 hours of exposure period. For knock-down bioassay (filter paper impregnation bioassay) different concentration of the methanolic extract and its various fractions (i.e. 0.0625, 0.125, 0.25, 0.5 and 1mg\/mL) were applied for 1 hour exposure period. The results were statistically analysed using standard deviation, probit analysis, and linear regression. The R2 values of larvae, pupae, and adult range from 0.4 to 0.99. The values of LC50 (concentration causing 50% mortality) for late 3rd instar larvae after 24 hours exposure period range from 93-1856.7 ppm, while LC90 values range from 424 -7635.5ppm. The values of LC50for pupae range form 1326.7-6818.4ppm and and values of LC90 range from 3667.3-17427.9ppm, respectively. The KDT50 range from 0.30 to 2.8% and KDT90 values range from1.2 to 110.8%, respectively. In conclusion, Justicia adhatoda may be effective for controlling populations of vector mosquito.<\/jats:p>","DOI":"10.1590\/1519-6984.248122","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T12:19:51Z","timestamp":1640002791000},"source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of indigenous plants\u2019 extracts for mosquitocidal activity against different stages of Culex quinquefasciatus say (Diptera: Culicidae)"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3588-7591","authenticated-orcid":false,"given":"A.","family":"Hussain","sequence":"first","affiliation":[{"name":"Shaheed Benazir Bhutto University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4779-6707","authenticated-orcid":false,"given":"I.","family":"Ilahi","sequence":"additional","affiliation":[{"name":"University of Malakand,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3180-8208","authenticated-orcid":false,"given":"H.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Shaheed Benazir Bhutto University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Niaz","sequence":"additional","affiliation":[{"name":"Abdul Wali Khan University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Masood","sequence":"additional","affiliation":[{"name":"Sardar Bahadur Khan Woman\u2019s University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5036-6786","authenticated-orcid":false,"given":"T.","family":"Khan","sequence":"additional","affiliation":[{"name":"Abdul Wali Khan University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Khan","sequence":"additional","affiliation":[{"name":"Bacha Khan University Charsadda,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Zaj\u0105c","sequence":"additional","affiliation":[{"name":"Medical University of Lublin,  Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Alkhaibari","sequence":"additional","affiliation":[{"name":"University of Tabuk,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4862-7668","authenticated-orcid":false,"given":"A. D.","family":"Alanazi","sequence":"additional","affiliation":[{"name":"Shaqra University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"key":"ref1","series-title":"Journal of Ethnobiology and Ethnomedicine","first-page":"25","volume-title":"Diversity and use of ethno-medicinal plants in the region of Swat, North Pakistan","volume":"9","author":"AKHTAR N.","year":"2013"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.22159\/ijpps.2017v9i8.19362","article-title":"Pupal emergence inhibition activity of Acalypha Indica leaf extract against dengue vector, Aedes Albopictus mosquito","volume":"9","author":"ASHWINI U.","year":"2017","journal-title":"International Journal of Pharmacy and Pharmaceutical Sciences"},{"year":"1934","series-title":"The fauna of British India including Ceylon and Burma.","author":"BARRAUD C.P.","key":"ref3"},{"issue":"1","key":"ref4","first-page":"73","article-title":"Tropical Pulmonary Eosinophilia and Filariasis in Pakistan","volume":"32","author":"BEG M.A.","year":"2001","journal-title":"South Eeast Asian Journal of Tropical Medicine."},{"issue":"2","key":"ref5","first-page":"123","article-title":"Insecticide resistance in mosquitoes: a pragmatic review","volume":"2","author":"BROWN A.W.","year":"1986","journal-title":"Journal of the American Mosquito Control Association"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"478","DOI":"10.4269\/ajtmh.2007.77.478","article-title":"Combined sewage overflows (CSO) are major urban breeding sites for Culex quinquefasciatus in Atlanta, Georgia","volume":"77","author":"CALHOUN L. 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