{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T17:35:22Z","timestamp":1775756122258,"version":"3.50.1"},"reference-count":40,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The present study aimed to investigate the beneficial of prepared black rice anthocyanins nano-composite (An-AgNps) against hepatotoxicity induced by methotrexate (MTX) in rats. Anthocyanins nano-composite was prepared by silver as the metallic ion reduction and were characterized by IR and SEM. The rats in our experiment were divided into five groups. Serum lipid profile, serum transaminases (ALT and AST), ALP, LDH, TBA, GSH and SOD were examined. The results show that SEM of An-AgNps has average particle size from 70 to 130nm. In the group treated with MTX; TC, TG, LDL-c, ALT, AST, ALP, LDH and TBA levels were significantly (P\u22640.05) increased than NC, while, HDL-c, SOD and GSH levels were significantly (P\u22640.05) decreased. On the other hand, An-AgNps + MTX treated groups were reversed the levels of all biomarkers similar to NC. In conclusion, the results show that An-AgNps has a protective effect on MTX-induced hepatotoxicity and oxidative stress.<\/jats:p>","DOI":"10.1590\/1519-6984.248726","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T22:10:26Z","timestamp":1646172626000},"source":"Crossref","is-referenced-by-count":6,"title":["Synthesis, characterization and protection effect of black rice anthocyanins nano-composite against hepatotoxicity induced by methotrexate in rats"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9246-2699","authenticated-orcid":false,"given":"M. A.","family":"Sheikha","sequence":"first","affiliation":[{"name":"Imam Abdulrahman Bin Faisal University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8056-2545","authenticated-orcid":false,"given":"N. A.","family":"Soheir","sequence":"additional","affiliation":[{"name":"Agricultural Research Center,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1065-9725","authenticated-orcid":false,"given":"F. M.","family":"SyragEldin","sequence":"additional","affiliation":[{"name":"Saudi Grains Organization \u2013 SAGO,  KSA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1093\/clinchem\/20.4.470","article-title":"Enzymatic determination of total serum cholesterol","volume":"20","author":"ALLAIN C.C.","year":"1974","journal-title":"Clinical Chemistry"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12951-016-0227-4","article-title":"Anthocyanins encapsulated by PLGA@ PEG nanoparticles potentially improved its free radical scavenging capabilities via p38\/JNK pathway against A\u03b2 1-42-induced oxidative stress","volume":"15","author":"AMIN F.U.","year":"2017","journal-title":"Journal of Nanobiotechnology"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/(SICI)1099-0844(1998120)16:4<283::AID-CBF801>3.0.CO;2-E","article-title":"Methotrexate: pentose cycle and oxidative stress","volume":"16","author":"BABIAK R.M.V.","year":"1998","journal-title":"Cell Biochemistry and Function"},{"issue":"11","key":"ref4","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1515\/cclm.1988.26.11.783","article-title":"General regression procedure for method transformation. application of linear regression procedures for method comparison studies in clinical chemistry, part III","volume":"26","author":"BABLOK W.","year":"1988","journal-title":"Clinical Chemistry and Laboratory Medicine"},{"key":"ref5","first-page":"S101","article-title":"Antifolates in rheumatoid arthritis: a hypothetical mechanism of action","volume":"11","author":"BAGGOTT J.E.","year":"1993","journal-title":"Clinical and Experimental Rheumatology"},{"issue":"9","key":"ref6","doi-asserted-by":"crossref","first-page":"3069","DOI":"10.1021\/nl801661w","article-title":"Reproducible comet assay of amorphous silica nanoparticles detects no genotoxicity","volume":"8","author":"BARNES C.A.","year":"2008","journal-title":"Nano Letters"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1159\/000469005","article-title":"Liver enzyme changes in a guinea-pig model of facial eczema (sporidesmiotoxicosis)","volume":"42","author":"BONNEFOI M.","year":"1989","journal-title":"Enzyme"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.jfca.2009.08.012","article-title":"Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits","volume":"23","author":"BOWEN-FORBES C.S.","year":"2010","journal-title":"Journal of Food Composition and Analysis"},{"issue":"22","key":"ref9","first-page":"2988","article-title":"Neuroprotective effects of carvacrol and pomegranate against methotrexate induced toxicity in rats","volume":"17","author":"CELIK F.","year":"2013","journal-title":"European Review for Medical and Pharmacological Sciences"},{"issue":"25","key":"ref10","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1039\/c3tb20476f","article-title":"New nanomaterials for the improvement of psoriatic lesions","volume":"1","author":"CRISAN M.","year":"2013","journal-title":"Journal of Materials Chemistry. 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