{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:27:39Z","timestamp":1792441659270,"version":"4.5.0"},"reference-count":43,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Zinc oxide nanoparticles were synthesized from the leaf extract of Brassica oleracea L. Acephala group (collard green) followed by their characterization using Scanning Electron Microscope (SEM), and Energy Dispersive X-ray (EDX). The antibacterial properties of zinc nanoparticles were tested against Gram-negative bacteria, Pseudomonas aeruginosa (ATCC \u00ae 9027\u2122), Escherichia coli (ATCC \u00ae 8739\u2122), Klebsiella pneumoniae (ATCC\u00ae BAA-1705\u2122) and Gram-positive bacteria, Staphylococcus aureus (ATCC \u00ae 6538\u2122) and Listeria monocytogenes (ATCC \u00ae 13932\u2122), at four different concentrations (50.00 \u00b5g\/ml, 100.00 \u00b5g\/ml, 500.00 \u00b5g\/ml and 1 mg\/ml) of zinc oxide nanoparticles suspension. Results revealed that the synthesized nanoparticles exhibit strong antibacterial effects against Pseudomonas aeruginosa, Listeria monocytogenes, Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli at 500.00 \u00b5g\/ml-1 mg\/ml concentrations. An increase in efficacy of nanoparticles with the decrease of their size was also evident. This is a first ever report on Brassica oleracea, L. based nanoparticles which demonstrates that 500.00 \u00b5g-1 mg\/ml conc. of zinc oxide nanoparticles have antibacterial activity against both Gram -ve and Gram +ve bacteria and have the potential to be considered as an antibacterial agent in future.<\/jats:p>","DOI":"10.1590\/1519-6984.259351","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T12:27:09Z","timestamp":1657542429000},"source":"Crossref","is-referenced-by-count":9,"title":["Brassica oleracea L. (Acephala Group) based zinc oxide nanoparticles and their efficacy as antibacterial agent"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2261-636X","authenticated-orcid":false,"given":"S.","family":"Wali","sequence":"first","affiliation":[{"name":"Hazara University, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0898-4062","authenticated-orcid":false,"given":"M.","family":"Zahra","sequence":"additional","affiliation":[{"name":"University of Peshawar, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4420-0587","authenticated-orcid":false,"given":"M. K.","family":"Okla","sequence":"additional","affiliation":[{"name":"King Saud University, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9177-4957","authenticated-orcid":false,"given":"H. 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antibacterial and antifungal activity","volume":"1211","author":"PILLAI A.M.","year":"2020","journal-title":"Journal of Molecular Structure"},{"issue":"4","key":"ref24","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s13204-015-0460-4","article-title":"Gold nanoparticles synthesized by Brassica oleracea (Broccoli) acting as antimicrobial agents against human pathogenic bacteria and fungi","volume":"6","author":"PIRUTHIVIRAJ P.","year":"2016","journal-title":"Applied Nanoscience"},{"key":"ref25","first-page":"195","author":"RAJAMANICKAM U.","year":"2012"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2139021","DOI":"10.1063\/1.2742324","article-title":"Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems","volume":"90","author":"REDDY K.M.","year":"2007","journal-title":"Applied Physics Letters"},{"issue":"15","key":"ref27","doi-asserted-by":"crossref","first-page":"3180","DOI":"10.1016\/j.physb.2010.04.042","article-title":"Structural, optical, photocatalytic and antibacterial activity of zinc oxide and manganese doped zinc oxide nanoparticles","volume":"405","author":"REKHA K.","year":"2010","journal-title":"Physica B, Condensed Matter"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1021\/cr030067f","article-title":"Nanostructures in biodiagnostics","volume":"105","author":"ROSI N.L.","year":"2005","journal-title":"Chemical Reviews"},{"issue":"Pt 3","key":"ref29","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.jenvman.2016.09.034","article-title":"Comparison between silver-and copper-modified zeolite-rich tuffs as microbicide agents for Escherichia coli and Candida albicans","volume":"183","author":"ROSSAINZ-CASTRO L.G.","year":"2016","journal-title":"Journal of Environmental 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Agriculture"},{"issue":"1-3","key":"ref36","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.colsurfa.2003.09.010","article-title":"Zinc oxide nanoparticles incorporated in ultrathin layer silicate films and their photocatalytic properties","volume":"230","author":"SZAB\u00d3 T.","year":"2003","journal-title":"Colloids and Surfaces A: Physicochemical and Engineering Aspects"},{"issue":"3","key":"ref37","first-page":"22","article-title":"Alginate membranes loaded with hyaluronic acid and silver nanoparticles to foster tissue healing and to control bacterial contamination of non-healing wounds","volume":"29","author":"TARUSHA L.","year":"2018","journal-title":"Journal of Materials Science: Materials in Medicine"},{"issue":"7","key":"ref38","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1166\/jnn.2018.15285","article-title":"Cellulose whiskers influence the morphology and antibacterial properties of silver nanoparticles 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