{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:27:06Z","timestamp":1777606026352,"version":"3.51.4"},"reference-count":41,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The natural phytochemical latex of Euphorbia milii var. hislopii is one of the most promising natural molluscicides for the control of Biomphalaria glabrata, and has been widely studied under laboratory conditions for selective control of schistosomiasis transmission. However, the effect of this product on B. glabrata infected by other helminths had not yet been investigated. The present study reports evaluation of the effect of E. milii var. hislopii latex on the survival and reproductive activity of B. glabrata infected by Angiostrongylus cantonensis. For this purpose, the following groups were formed: control (C), exposed (E), infected (I) and infected and exposed for different time intervals (1 day - I+E-1D, 7 days - I+E-7D, 14 days - I+E-14D, 21 days - I+E-21D and 28 days - I+E-28D). The experimental infection was performed with L1 larvae of A. cantonensis and exposure to 0.08 mg\/L (LC50) of E. milii latex for a period of 24 hours. We analyzed the effects of E. milii latex on the survival of snails during four weeks, reproductive parameters and possible histophysiopathological changes in the gonad and albumen gland of the snails. Regarding survival, there was reduction of more than 50% in the groups exposed to latex (E and I + E) compared to the control group. As for the number of ovigerous masses, eggs, and average number of hatched snails, significant increases were observed in the I+E-1D group at the 4th week compared to the control group and the other weeks in the same group. Angiostrongylus cantonensis larvae were observed in the gonad and albumen gland from day 21 and 28 of infection in groups I and I+E, respectively, with granuloma-like formation. At these observation periods and in these groups, an increase in galactogen was observed in the albumen gland, which influenced egg laying, suggesting the existence of a fecundity compensation mechanism phenomenon. It was possible to conclude that both stressors \u2013 A. cantonensis infection and exposure to E. milii latex \u2013 directly influenced the survival and reproductive parameters of B. glabrata.<\/jats:p>","DOI":"10.1590\/1519-6984.259628","type":"journal-article","created":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T09:06:27Z","timestamp":1661504787000},"source":"Crossref","is-referenced-by-count":3,"title":["Reproductive alterations of Biomphalaria glabrata (Say, 1818) infected with Angiostrongylus cantonensis (Chen, 1935) and exposed to Euphorbia milii var. hislopii latex"],"prefix":"10.1590","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5638-9025","authenticated-orcid":false,"given":"A. C.","family":"Alberto-Silva","sequence":"first","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro, Brasil; Funda\u00e7\u00e3o Oswaldo Cruz, Brasil; Universidade Federal Rural do Rio de Janeiro, Brasil; Funda\u00e7\u00e3o Oswaldo Cruz, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8841-2557","authenticated-orcid":false,"given":"J. S.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0712-3874","authenticated-orcid":false,"given":"E. M.","family":"Mota","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8648-4530","authenticated-orcid":false,"given":"F. G.","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8370-2814","authenticated-orcid":false,"given":"J.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro, Brasil; Universidade Federal Rural do Rio de Janeiro, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5575-2272","authenticated-orcid":false,"given":"C. C.","family":"Mello-Silva","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2022]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1590\/S1516-05722010000100013","article-title":"Avalia\u00e7\u00e3o da atividade moluscicida do l\u00e1tex de tr\u00eas esp\u00e9cies de Euphorbia (Euphorbiaceae) sobre Leptinaria unilamellata d\u2019Orbigny, 1835 (Gastropoda-Subulinidae)","volume":"12","author":"AFONSO-NETO I.S.","year":"2010","journal-title":"Revista Brasileira de Plantas Medicinais"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","DOI":"10.1590\/0001-3765202020190298","article-title":"Behavioral response of Biomphalaria glabrata exposed to a sublethal concentration of Euphorbia milii var. hislopii latex","volume":"92","author":"ALBERTO-SILVA A.C.","year":"2020","journal-title":"Anais da Academia Brasileira de Ci\u00eancias"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.exppara.2015.03.004","article-title":"Changes in the locomotory and reproductive behavior of Biomphalaria glabrata infected with Schistosoma mansoni.","volume":"153","author":"ALBERTO-SILVA A.C.","year":"2015","journal-title":"Experimental Parasitology"},{"key":"ref4","first-page":"38","article-title":"Angiostrongyliasis cantonensis (eosinophilic meningitis): historical events in its recognition as a new parasitic disease of man","volume":"78","author":"ALICATA J.E.","year":"1988","journal-title":"Journal of the Washington Academy of Sciences"},{"key":"ref5","first-page":"127","article-title":"Schistosoma mansoni: phytochemical effect on aquatic life cycle","volume":"5","author":"AUGUSTO R.C.","year":"2015","journal-title":"Journal of Veterinary Medicine"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"13","DOI":"10.24039\/rnh2016101724","article-title":"First electron probe x-ray microanalysis of the elemental composition of the lyophilized l\u00e1tex of Euphorbia milii var. hislopii and its impact in the male Schistosoma mansoni.","volume":"10","author":"AUGUSTO R.C.","year":"2016","journal-title":"Neotropical Helminthology"},{"issue":"7","key":"ref7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005789","article-title":"Double impact: natural molluscicide for schistosomiasis vector control also impedes development of Schistosoma mansoni cercariae into adult parasites","volume":"11","author":"AUGUSTO R.C.","year":"2017","journal-title":"PloS Neglected Tropical Diseases"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1558-5646.1975.tb00213.x","article-title":"Host castration as a parasitic strategy","volume":"29","author":"BAUDOIN M.","year":"1975","journal-title":"Evolution"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jip.2013.10.005","article-title":"Biochemical and histopathological alterations in Biomphalaria glabrata due to co-infection by Angiostrongylus cantonensis and Echinostoma paraensei.","volume":"115","author":"BONFIM T.C.S.","year":"2014","journal-title":"Journal of Invertebrate Pathology"},{"issue":"7","key":"ref10","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1590\/S0074-02762007000700018","article-title":"First record of molluscs naturally infected with Angiostrongylus cantonensis (Chen, 1935) (Nematoda: Metastrongylidae) in Brazil","volume":"102","author":"CALDEIRA R.L.","year":"2007","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1093\/ajcp\/59.3.365","article-title":"Formalin fixation for electron microscopy: a re-evaluation","volume":"59","author":"CARSON F.L.","year":"1973","journal-title":"American Journal of Clinical Pathology"},{"issue":"2","key":"ref12","first-page":"77","article-title":"Determination of LD50 of the latex of Euphorbia splenders var. hislopii N.E.B (syn. 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intermediate host of Fasciola hepatica Linnaeus, 1758 (Trematoda: Fasciolidae). 1- Test in laboratory","volume":"98","author":"VASCONCELLOS M.C.","year":"2003","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1590\/S0074-02761986000400017","article-title":"Latex of \u201cCoroa de Cristo\u201d (Euphorbia splendens): an effective molluscicide","volume":"81","author":"VASCONCELLOS M.C.","year":"1986","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"issue":"916","key":"ref40","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1086\/282461","article-title":"Natural selection, the costs of reproduction, and a refinement of Lack\u2019s principle","volume":"100","author":"WILLIAMS G.C.","year":"1966","journal-title":"American Naturalist"},{"key":"ref41","series-title":"Report of the scientific working group on plant molluscicide","year":"1983"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Altera\u00e7\u00f5es na atividade reprodutiva de Biomphalaria glabrata (Say, 1818) infectada com Angiostrongylus cantonensis (Chen, 1935) e exposta ao l\u00e1tex de Euphorbia milii var. hislopii"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842022000100717&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T16:59:01Z","timestamp":1727888341000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842022000100717&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":41,"alternative-id":["S1519-69842022000100717"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.259628","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]},"article-number":"e259628"}}