{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:02:30Z","timestamp":1790218950625,"version":"4.1.0"},"reference-count":50,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Acute Giardia infections often cause diarrhea and stomach upset. Chronic infections can lead to malnutrition, micronutrient deficiencies, malabsorption and weight loss. This study assessed the prevalence of G. lambia infection and assessed associated risk factors among immunocompomised patients undergoing chemotherapeutic treatment in southern Brazil. A total of 110 immunocompromised patients in Pelotas, RS, Brazil, consented to participate in this study and were recruited. Socioeconomic and epidemiological profile of patients was collected by questionnaire. The prevalence for Giardia were determined through microscopy by the centrifugation-flotation technique using stool samples of every patient. In addition, the genetic characterization of the parasite was carried out by amplifying and sequencing the glutamate dehydrogenase (gdh) gene. By microscopy, the prevalence of giardiasis was 17.3% (19\/110). Furthermore, the DNA sequences revealed that 7 (36.8%) out of 19 isolates belonged to assemblage B, while 6 of them (31.6%) belonged to assemblage C, 5 (26.3%) to assemblage A and 1 (5.3%) to assemblage D. Risk factors (p \u2264 0.05) for giardiasis were schooling level (OR=8.0 (1.02 \u2013 62.91) sharing a house with more than three people (OR=14.1 (3.77 \u2013 52.51), water sources (OR=38.9 (10.4 \u2013 145.7), sewage treatment (OR=14.2 (3.1 \u2013 65.5), waste destination (OR=7.44 (2.0 \u2013 27.3), owning pets (OR=4.6 (1.0 \u2013 21.2) and cultivating a vegetable garden (OR=4.2 (1.3 \u2013 13.6). The prevalence of G. lamblia in immunocompromised patients was considered elevate with the identification of four assemblage of the parasite (A, B, C and D).<\/jats:p>","DOI":"10.1590\/1519-6984.265055","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T12:11:19Z","timestamp":1670587879000},"source":"Crossref","is-referenced-by-count":10,"title":["Molecular characterization of Giardia lamblia and risk factors for giardiasis among immunocompromised patients in southern Brazil"],"prefix":"10.1590","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1622-9219","authenticated-orcid":false,"given":"S. T.","family":"Jeske","sequence":"first","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3113-3426","authenticated-orcid":false,"given":"M. R. P.","family":"Macedo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3662-6385","authenticated-orcid":false,"given":"T.","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7152-1765","authenticated-orcid":false,"given":"\u00cd. F.","family":"Leon","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6822-501X","authenticated-orcid":false,"given":"N. B.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1774-0059","authenticated-orcid":false,"given":"S.","family":"Borsuk","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1614-8865","authenticated-orcid":false,"given":"M.","family":"Villela","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2022]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.4314\/ejhs.v28i2.6","article-title":"Prevalence of opportunistic intestinal parasites and associated factors among HIV 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J.W.B.","year":"2006","journal-title":"International Journal for Parasitology"},{"issue":"5","key":"ref48","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.ijpara.2018.12.006","article-title":"Risk factors for infection with soil-transmitted helminths during an integrated community level water, sanitation, and hygiene and deworming intervention in Timor-Leste","volume":"49","author":"VAZ NERY S.","year":"2019","journal-title":"International Journal for Parasitology"},{"issue":"8","key":"ref49","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1590\/S1413-81232011000900016","article-title":"Estudo da associa\u00e7\u00e3o entre fatores socioambientais e preval\u00eancia de parasitose intestinal em \u00e1rea perif\u00e9rica da cidade de Manaus (AM, Brasil)","volume":"16","author":"VISSER S.","year":"2011","journal-title":"Ciencia & Saude Coletiva"},{"issue":"1","key":"ref50","first-page":"19","article-title":"Prevalence of intestinal parasites and socioeconomic 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Brasil"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842022000100770&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T12:12:13Z","timestamp":1670587933000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842022000100770&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":50,"alternative-id":["S1519-69842022000100770"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.265055","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]},"article-number":"e265055"}}