{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T20:55:12Z","timestamp":1766609712592,"version":"3.41.2"},"reference-count":77,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Currently, available fish anesthetics can produce important side effects, including respiratory arrest and distress. Easy-to-implement alternatives with low toxicity are needed to ensure fish health as well as to help artisanal fisheries and fish sellers in handling and transporting fishes, and native plants seems to be the best alternative. We aimed to implement an anesthetic protocol using crude ethanolic extracts from flowers and leaves of two Amazonian plants, the Acmella oleracea and Piper alatabaccum. We first tested the extracts for anesthesia, using the zebrafish as model. Even though in some treatments the animals apparently entered deep anesthesia, many of them presented aberrant behaviors and even died. Thus, we performed new experiments testing the extracts effects on seizure-like behaviors of the fish. Only the leaf extract of A. oleracea has potential effects for fish anesthesia. Both the flower extract from this plant and the leaf extract from P. alatabaccum induced seizure-like behavior in the animals. In conclusion, besides bringing a possible new anesthetic protocol for fish, our work draws attention for the neurotoxic effects the anesthetic solutions may cause, since several studies defend other Piper species as anesthetic for fish and A. oleracea flowers\u2019 extract was already pointed as fish anesthetic.<\/jats:p>","DOI":"10.1590\/1519-6984.266010","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T15:07:27Z","timestamp":1665155247000},"source":"Crossref","is-referenced-by-count":3,"title":["Anesthesia or seizure-like behavior? The effects of two Amazonian plants, Acmella oleracea and Piper alatabaccum in zebrafish (Danio rerio)"],"prefix":"10.1590","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7871-9950","authenticated-orcid":false,"given":"M.","family":"Leite","sequence":"first","affiliation":[{"name":"Universidade Federal do Sul e Sudeste do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0566-8304","authenticated-orcid":false,"given":"H.","family":"Tercya","sequence":"additional","affiliation":[{"name":"Universidade Federal do Sul e Sudeste do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2641-6018","authenticated-orcid":false,"given":"B. G.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Universidade Federal do Sul e Sudeste do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2632-5888","authenticated-orcid":false,"given":"J.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Universidade Federal do Sul e Sudeste do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5537-5198","authenticated-orcid":false,"given":"R.","family":"Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal do Sul e Sudeste do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9001-5980","authenticated-orcid":false,"given":"B. P. 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H.","family":"Siqueira-Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal do Sul e Sudeste do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2022]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1577\/1548-8667(200006)012<0157:PAIEOA>2.0.CO;2","article-title":"Physiological and immunological effects of adjuvanted Aeromonas salmonicida vaccines on juvenile rainbow trout.","volume":"12","author":"ACKERMAN P.A.","year":"2000","journal-title":"Journal of Aquatic Animal Health"},{"year":"2004","series-title":"Efeitos do eugenol sobre o m\u00fasculo liso traqueal de cobaio.","author":"ALMEIDA M.A.","key":"ref2"},{"year":"2009","series-title":"Variabilidade qu\u00edmica em \u00f3leos essenciais de esp\u00e9cies de Piper da Amaz\u00f4nia.","author":"ANDRADE 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