{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:25:26Z","timestamp":1782401126140,"version":"3.54.5"},"reference-count":38,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation. As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August. The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach \u00abDomestos\u00bb in the dilution of H2O 1:9 (10 min). For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide \u201cTopaz\u201d (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) \u201cDomestos\u201d in the dilution of H2O 1:5 (20 min). For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) \u201cDomestos\u201d in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg\/l \u041a\u041cnO4 - 30 min. Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose. For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose. For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose. Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry. Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.<\/jats:p>","DOI":"10.1590\/1519-6984.266975","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T15:07:10Z","timestamp":1684163230000},"source":"Crossref","is-referenced-by-count":8,"title":["Micropropagation of berry crops for creation of germplasm cryobanks"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6476-1007","authenticated-orcid":false,"given":"T.","family":"Turdiyev","sequence":"first","affiliation":[{"name":"Institute of Plant Biology and Biotechnology,  Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8315-1490","authenticated-orcid":false,"given":"I.","family":"Kovalchuk","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology and Biotechnology,  Kazakhstan; Kazakh Research Institute of Fruit and Vegetable Growing,  Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0447-1884","authenticated-orcid":false,"given":"Z.","family":"Mukhitdinova","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology and Biotechnology,  Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0260-3270","authenticated-orcid":false,"given":"O.","family":"Hunger","sequence":"additional","affiliation":[{"name":"German Agricultural Society,  Deutschland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1185-2087","authenticated-orcid":false,"given":"S.","family":"Frolov","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology and Biotechnology,  Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4933-4151","authenticated-orcid":false,"given":"B.","family":"Kabylbekova","sequence":"additional","affiliation":[{"name":"Kazakh Research Institute of Fruit and Vegetable Growing,  Kazakhstan; Abai Kazakh National Pedagogical University,  Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"3","key":"ref1","first-page":"375","article-title":"Evaluation of nutritional composition and in vitro antioxidant and antibacterial activities of Codium intricatum Okamura from Ilocos Norte (Philippines)","volume":"13","author":"ARGUELLES E.D.","year":"2020","journal-title":"Jordan Journal of Biological Sciences"},{"key":"ref2","series-title":"Onions and allied crops.","first-page":"177","volume-title":"Conservation of genetic resources.","author":"ASTLEY D.","year":"2018"},{"issue":"7","key":"ref3","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.3390\/ijms18071427","article-title":"Plants under stress: involvement of auxin and cytokinin","volume":"18","author":"BIELACH A.","year":"2017","journal-title":"International Journal of Molecular Sciences"},{"key":"ref4","series-title":"BGCI","year":"2022"},{"key":"ref5","series-title":"Current frontiers in cryopreservation.","first-page":"415","volume-title":"Proline and the cryopreservation of plant tissues: functions and practical applications.","author":"BURRITT D.J.","year":"2012"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1590\/1678-4685-gmb-2017-0020","article-title":"Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks","volume":"41","author":"DANTAS A.F.","year":"2018","journal-title":"Genetics and Molecular Biology"},{"issue":"7","key":"ref7","first-page":"317","article-title":"Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv Beurr\u00e9 Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen: effects of previous cold hardening","volume":"310","author":"DEREUDDRE J.","year":"1990","journal-title":"Comptes Rendus de l\u2019Acad\u00e9mie des Sciences. 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