{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T13:56:34Z","timestamp":1764251794092,"version":"3.41.2"},"reference-count":39,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Emergence of plasmid mediated colistin and extended spectrum \u03b2-lactamases (ESBL) resistant genes has been impacted the efficacy of colistin and \u03b2-lactams drugs like 3rd, 4th generation cephalosporin. Current study was aimed to investigate antimicrobial resistance genes (ARGs) among Escherichia coli isolates from meat producing commercial broilers in Pakistan. Two hundred (n=200) fecal samples were collected during January-2018 to August-2019. For isolation of E. coli, pink colonies on MacConkey agar were transferred to EMB agar. Metallic sheen color colonies were tested biochemically using API-20E kit. The molecular identification of E. coli (n=153) was targeted by amplification of uid gene through polymerase chain reaction (PCR) and different ARGs i.e. gentamicin, streptomycin, tetracycline, colistin, \u03b2-lactams drugs, quinolone and ampicillin followed by sequence analysis. Genotypically, followed by phenotypically of resistant ARGs of isolated PCR-confirmed E. coli (153) shoed resistant against gentamicin (aac(3)-IV), streptomycin (aadA1), tetracycline (tetA), colistine (mcr-1), ampicillin (bla-TEM) and bla-CTX-M were 86%, 88%, 86%, 88%, 83% &amp; 77% respectively. 33\/38 (86%) of the isolate was positive for quinolone resistance. Colistine (mcr-1), ESBLs (bla-TEM) and (bla-CTX-M) resistance genes were 88%, 83% and 77% respectively. About 33 isolated E. coli harbored the both mcr-1 and ESBLs genes. All of E. coli isolates were found sensitive to ceftriaxone (CTX-30) and imipenem (IMP-10). The Isolated E. coli showed single or multi clade decadency. The E. coli and ARGs sequences showed single or multi clade decadency. This is first comprehensive study from Pakistan that described the molecular evidences of ARGs and their co-existence in single isolates originated from commercial poultry. Commercial chicken (Broilers) can act as melting pot of antibiotic resistance genes for human being. It is alarming situation for surveillance of antibiotic resistance program because of more regulated prescription of antimicrobial agents in Pakistan<\/jats:p>","DOI":"10.1590\/1519-6984.267494","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T15:11:14Z","timestamp":1675264274000},"source":"Crossref","is-referenced-by-count":6,"title":["Molecular mechanism of antimicrobial co-resistance Colistin (mcr-1) and ESBLs genes among Escherichia coli isolates from commercial chickens in Pakistan"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1922-6101","authenticated-orcid":false,"given":"W. A.","family":"Madni","sequence":"first","affiliation":[{"name":"Government College University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5460-5780","authenticated-orcid":false,"given":"M.","family":"Mohsin","sequence":"additional","affiliation":[{"name":"University of Agriculture,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4673-2579","authenticated-orcid":false,"given":"Z.","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Government College University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7775-8878","authenticated-orcid":false,"given":"S.","family":"Muzammil","sequence":"additional","affiliation":[{"name":"Government College University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0895-7312","authenticated-orcid":false,"given":"M. A.","family":"Zahoor","sequence":"additional","affiliation":[{"name":"Government College University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Asif","sequence":"additional","affiliation":[{"name":"Qarshi University Lahore,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"9","key":"ref1","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.3855\/jidc.4222","article-title":"Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria","volume":"8","author":"ADELOWO O.O.","year":"2014","journal-title":"Journal of Infection in Developing Countries"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1093\/jac\/dkh221","article-title":"New aminoglycoside acetyltransferase gene, aac (3)-Id, in a class 1 integron from a multiresistant strain of Vibrio fluvialis isolated from an infant aged 6 months","volume":"53","author":"AHMED A.M.","year":"2004","journal-title":"The Journal of Antimicrobial Chemotherapy"},{"issue":"8","key":"ref3","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.14202\/vetworld.2018.1082-1088","article-title":"Antimicrobial resistance genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics","volume":"11","author":"AMER M.M.","year":"2018","journal-title":"Veterinary World"},{"issue":"4","key":"ref4","first-page":"977","article-title":"Spread of antimicrobial resistance Escherichia coli from broiler to human population","volume":"56","author":"AMIR M.","year":"2019","journal-title":"Pakistan Journal of Agricultural Sciences"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12941-016-0174-9","article-title":"Genetic elements associated with antimicrobial resistance among avian pathogenic Escherichia coli.","volume":"15","author":"AWAD A.","year":"2016","journal-title":"Annals of Clinical Microbiology and Antimicrobials"},{"issue":"3","key":"ref6","first-page":"118","article-title":"Susceptibility and multidrug resistance patterns of Escherichia coli Isolated from cloacal swabs of live broiler chickens in Bangladesh","volume":"8","author":"AZAD M.A.R.A.","year":"2019","journal-title":"Pathogens (Basel, Switzerland)"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.17221\/428\/2014-CJFS","article-title":"ESBL-producing bacteria and MRSA isolated from poultry and turkey products imported from Italy","volume":"33","author":"BENINATI C.","year":"2015","journal-title":"Czech Journal of Food Sciences"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"S347","DOI":"10.1038\/sj.bjp.0707607","article-title":"Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria","volume":"153","author":"BENNETT P.M.","year":"2008","journal-title":"British Journal of Pharmacology"},{"issue":"5331","key":"ref9","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1126\/science.277.5331.1453","article-title":"The complete genome sequence of Escherichia coli K-12","volume":"277","author":"BLATTNER F.R.","year":"1997","journal-title":"Science"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ijfoodmicro.2019.02.006","article-title":"Revealing mcr-1-positive ESBL-producing Escherichia coli strains among Enterobacteriaceae from food-producing animals (bovine, swine and poultry) and meat (bovine and swine), Portugal, 2010-2015","volume":"296","author":"CLEMENTE L.","year":"2019","journal-title":"International Journal of Food Microbiology"},{"issue":"Pt 5","key":"ref11","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1099\/jmm.0.016188-0","article-title":"A novel IS26 structure surrounds blaCTX-M genes in different plasmids from German clinical Escherichia coli isolates","volume":"59","author":"CULLIK A.","year":"2010","journal-title":"Journal of Medical Microbiology"},{"issue":"3","key":"ref12","first-page":"94","article-title":"Antibiotic Resistance pattern of Escherichia coli isolated from diarrhoeic and Non-diarrhoeic under five children in Kano, Nigeria","volume":"4","author":"DABO N.T.","year":"2019","journal-title":"Journal of Microbiology and Biotechnology"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1186\/s13104-019-4825-8","article-title":"Identification phenotypic and genotypic characterization of biofilm formation in Escherichia coli isolated from urinary tract infections and their antibiotics resistance","volume":"12","author":"DAVARI ABAD E.","year":"2019","journal-title":"BMC Research Notes"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1186\/s12866-018-1322-5","article-title":"Antibiotic-resistant Escherichia coli from retail poultry meat with different antibiotic use claims","volume":"18","author":"DAVIS G.S.","year":"2018","journal-title":"BMC Microbiology"},{"issue":"1","key":"ref15","first-page":"19","article-title":"Emerging & re-emerging infections in India: an overview","volume":"138","author":"DIKID T.","year":"2013","journal-title":"The Indian Journal of Medical Research"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"859","DOI":"10.14202\/vetworld.2018.859-867","article-title":"Molecular characterization and antibiotic resistance patterns of avian fecal Escherichia coli from turkeys, geese, and ducks","volume":"11","author":"DUBE N.","year":"2018","journal-title":"Veterinary World"},{"year":"2007","series-title":"Antimicrobial chemotherapy","author":"GREENWOOD 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W3110","volume":"2","author":"HAYASHI K.","year":"2006","journal-title":"Molecular Systems Biology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.3389\/fmicb.2017.02120","article-title":"Risk of transmission of antimicrobial resistant Escherichia coli from commercial broiler and free-range retail chicken in India","volume":"8","author":"HUSSAIN A.","year":"2017","journal-title":"Frontiers in Microbiology"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"141","DOI":"10.14202\/vetworld.2019.141-145","article-title":"The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt","volume":"12","author":"IBRAHIM W.A.","year":"2019","journal-title":"Veterinary World"},{"issue":"1-2","key":"ref24","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.ijfoodmicro.2010.06.025","article-title":"Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients","volume":"142","author":"JAKOBSEN L.","year":"2010","journal-title":"International Journal of Food Microbiology"},{"issue":"2","key":"ref25","first-page":"233","article-title":"Differentiation of common gram negative pathogens by PCR-Ribotyping","volume":"23","author":"JAMIL M.","year":"2007","journal-title":"Pakistan Journal of Medical Sciences"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"KUMAR S.","year":"2018","journal-title":"Molecular Biology and Evolution"},{"issue":"17","key":"ref27","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.4103\/0366-6999.213407","article-title":"Trends in antimicrobial resistance against enterobacteriaceae strains isolated from blood: a 10-year epidemiological study in mainland China (2004-2014)","volume":"130","author":"LIU X.J.","year":"2017","journal-title":"Chinese Medical Journal"},{"key":"ref28","doi-asserted-by":"crossref","DOI":"10.1177\/11786361221093278","article-title":"Safety of retailed poultry: analysis of antibiotic resistance in Escherichia coli from raw chicken and poultry fecal matter from selected farms and retail outlets in Accra, Ghana","volume":"15","author":"MENSAH G.I.","year":"2022","journal-title":"Microbiology Insights"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2476","DOI":"10.3389\/fmicb.2017.02476","article-title":"High prevalence of CTX-M-15-Type ESBL-producing E. coli from migratory avian species in Pakistan","volume":"8","author":"MOHSIN M.","year":"2017","journal-title":"Frontiers in Microbiology"},{"issue":"4","key":"ref30","doi-asserted-by":"crossref","first-page":"193","DOI":"10.17221\/5916-VETMED","article-title":"Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran","volume":"57","author":"MOMTAZ H.","year":"2012","journal-title":"Veterinarni Medicina"},{"issue":"4","key":"ref31","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.diagmicrobio.2018.10.016","article-title":"First report of mcr-1-harboring Salmonella enterica serovar Schwarzengrund isolated from poultry meat in Brazil","volume":"93","author":"MORENO L.Z.","year":"2019","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1186\/1756-0500-7-627","article-title":"A cross-sectional study on the microbiological quality and safety of raw chicken meats sold in Nairobi, Kenya","volume":"7","author":"ODWAR J.A.","year":"2014","journal-title":"BMC Research Notes"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1128\/JCM.01319-06","article-title":"Effects of phenotype and genotype on methods for detection of extended-spectrum-beta-lactamase-producing clinical isolates of Escherichia coli and Klebsiella pneumoniae in Norway","volume":"45","author":"TOFTELAND S.","year":"2007","journal-title":"Journal of Clinical Microbiology"},{"issue":"7","key":"ref34","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.apjtb.2017.07.002","article-title":"Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains","volume":"7","author":"VUTHY Y.","year":"2017","journal-title":"Asian Pacific Journal of Tropical Biomedicine"},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.15666\/aeer\/1702_30493063","article-title":"Potential dissemination of antimicrobial resistance from small scale poultry slaughter in Pakistan","volume":"17","author":"WASEEM H.","year":"2019","journal-title":"Applied Ecology and Environmental Research"},{"issue":"5","key":"ref36","first-page":"e01204","article-title":"Colistin resistance gene mcr-1 and its variant in Escherichia coli isolates from chickens in China","volume":"61","author":"YANG Y.Q.","year":"2017","journal-title":"Antimicrobial Agents and Chemotherapy"},{"issue":"10","key":"ref37","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.14202\/vetworld.2017.1167-1172","article-title":"Phenotypic and genotypic characterization of antimicrobial susceptibility of avian pathogenic Escherichia coli isolated from broiler chickens","volume":"10","author":"YOUNIS G.","year":"2017","journal-title":"Veterinary World"},{"issue":"19","key":"ref38","first-page":"4467","article-title":"Antimicrobial susceptibilities of avian Escherichia coli isolates in Tabriz, Iran","volume":"11","author":"ZAKERI A.","year":"2012","journal-title":"African Journal of Biotechnology"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"3705","DOI":"10.1038\/s41598-018-22084-4","article-title":"Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal\/oropharyngeal and anal\/cloacal swabs from pigs and poultry","volume":"8","author":"ZHANG J.","year":"2018","journal-title":"Scientific Reports"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Mecanismo molecular de corresist\u00eancia antimicrobiana de Colistina (mcr-1) e genes ESBLs entre isolados de Escherichia coli em frangos comerciais no Paquist\u00e3o"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842024000100429&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T15:11:50Z","timestamp":1675264310000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842024000100429&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":39,"alternative-id":["S1519-69842024000100429"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.267494","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e267494"}}