{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:08:18Z","timestamp":1753888098422,"version":"3.41.2"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Leishmaniasis is an anthropozoonosis transmitted by vectors, with dogs being the main domestic reservoirs. Brazil is one of the countries most affected by this disease, and it has been described in humans and dogs in every region in the country. In the northern region leishmaniasis cases in humans have been described in more than 100 municipalities in the State, including the capital, Bel\u00e9m. This study involves two cases of canine visceral leishmaniasis in which the animals developed clinical signs compatible with the disease in urban areas in Bel\u00e9m, the Par\u00e1 state capital. The diagnosis was confirmed via polymerase chain reaction (PCR) to detect SSUr-rDNA and kDNA of Leishmania sp. and Leishmania infantum, respectively. In one of the cases the animal died and in the other the animal underwent treatment with medicines prescribed for dogs. Through this treatment, parasitemia in the second animal has been kept under control and is being monitored through molecular tests. Previously, no canine cases had been notified from urban neighborhoods in the city of Bel\u00e9m, but only on the island of Cotijuba, at a distance of 29 kilometers from the city. Cases of canine and human leishmaniasis have been recorded close to the capital, Bel\u00e9m, which has areas of conserved vegetation and where the presence of disease vectors has been described. Thus, as has been done in several other Brazilian cities, this study uses clinical and laboratory findings to confirm the presence of autochthonous cases of canine visceral leishmaniasis in the city of Bel\u00e9m.<\/jats:p>","DOI":"10.1590\/1519-6984.267617","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T09:41:46Z","timestamp":1686303706000},"source":"Crossref","is-referenced-by-count":2,"title":["First molecular description of autochthonous urban cases of canine visceral leishmaniasis in the city of Bel\u00e9m, Par\u00e1, Brazil"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4575-5872","authenticated-orcid":false,"given":"G. R.","family":"Galv\u00e3o","sequence":"first","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2221-1995","authenticated-orcid":false,"given":"E. C.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2304-7162","authenticated-orcid":false,"given":"L. G. S.","family":"Moura","sequence":"additional","affiliation":[{"name":"Clinica CENTROPET,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0658-2075","authenticated-orcid":false,"given":"R. R.","family":"Virgolino","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7899-3650","authenticated-orcid":false,"given":"A. M. P.","family":"Neves","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9075-9947","authenticated-orcid":false,"given":"D. C. F.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"6","key":"ref1","first-page":"978","article-title":"Standardization of DNA extraction with NaCl from oral mucosa cells: application in PROP1 gene study","volume":"49","author":"ABR\u00c3O M.G.","year":"2005","journal-title":"Arquivos Brasileiros de Endocrinologia & Metabologia"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0035671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"ALVAR J.","year":"2012","journal-title":"PLoS One"},{"edition":"5.","year":"2011","series-title":"Sistema nacional de vigil\u00e2ncia em sa\u00fade: relat\u00f3rio de situa\u00e7\u00e3o: Par\u00e1","key":"ref3"},{"year":"2014","series-title":"Manual de vigil\u00e2ncia e 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Brasil"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842023000100800&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T09:42:20Z","timestamp":1686303740000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842023000100800&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":37,"alternative-id":["S1519-69842023000100800"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.267617","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e267617"}}