{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T21:03:24Z","timestamp":1763499804663,"version":"3.41.2"},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Molecular appraoch for identification of unknown species by using Cytochrome b gene is an effective and reliable as compared with morphological based identification. For DNA barcoding universal molecular genes were used to identify the species. Cytochrome b is a specific gene used for identification purpose. DNA barcoding is a reliable and effective method compared to the different traditional morphological methods of specie identification. So,in the present study which was conducted to identify the species, a total of 50 fish samples were collected from five different sites. DNA was extracted by using the Phenol Chloroform method from muscle tissue. Five sequences were sequenced (one from each site), analyzed, and identified specific species as Pangasius pangasius. Identified sequences were variable in length from 369 bp (Site 1), 364 bp (Site 2), 364 bp (Site 3), 352 bp (Site 4), and 334 bp (Site 5). Identity matches on the NCBI database confirmed the specific specie as P. pangasius. A distancing tree was drawn to show maximum likelihood among the same and different species. Yet, in many cases fishes on diverse development stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative tool for species identification and phylogenetic study. This work intends to provide an updated and extensive overview on the DNA based methods for fish species identification by using Cytochrome b gene as targeted markers for identification purpose.<\/jats:p>","DOI":"10.1590\/1519-6984.268001","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T15:14:26Z","timestamp":1671635666000},"source":"Crossref","is-referenced-by-count":2,"title":["Molecular based identification and phylogenetic relationship by using cytochrome b gene of Pangasius pangasius"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1323-3490","authenticated-orcid":false,"given":"S.","family":"Hassan","sequence":"first","affiliation":[{"name":"Bahauddin Zakariya University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5311-3557","authenticated-orcid":false,"given":"M.","family":"Naeem","sequence":"additional","affiliation":[{"name":"Bahauddin Zakariya University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9446-9234","authenticated-orcid":false,"given":"M. Farhan","family":"Nasir","sequence":"additional","affiliation":[{"name":"University of Education,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7254-8126","authenticated-orcid":false,"given":"P.","family":"Riaz","sequence":"additional","affiliation":[{"name":"Bahauddin Zakariya University,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3182-6441","authenticated-orcid":false,"given":"M. N.","family":"Khan","sequence":"additional","affiliation":[{"name":"University of Jhang,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1534-4239","authenticated-orcid":false,"given":"I.","family":"Atiq","sequence":"additional","affiliation":[{"name":"Ghazi University Dera Ghazi Khan,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"8579","DOI":"10.1038\/s41598-018-26976-3","article-title":"DNA Barcoding of freshwater fishes of Indo-Myanmar biodiversity hotspot","volume":"8","author":"BARMAN A.S.","year":"2018","journal-title":"Scientific Reports"},{"issue":"1-3","key":"ref2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/S0379-0738(03)00219-6","article-title":"A method for simultaneous RNA and DNA isolation from dried blood and semen 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An improved method for DNA extraction from fish fins and scales","volume":"138","author":"WASKO A.P.","year":"2003","journal-title":"Hereditas"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Identifica\u00e7\u00e3o de base molecular e rela\u00e7\u00e3o filogen\u00e9tica usando o gene citocromo b de Pangasius pangasius"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842024000100411&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T15:14:50Z","timestamp":1671635690000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842024000100411&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":23,"alternative-id":["S1519-69842024000100411"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.268001","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e268001"}}