{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T01:54:48Z","timestamp":1771466088383,"version":"3.50.1"},"reference-count":41,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Rhodnius neglectus is a wild triatomine, vector of the protozoan Trypanosoma cruzi, which causes Chagas\u2019 disease, and feeds on the blood of small mammals, being essential for its growth and reproduction. Accessory glands of the female reproductive tract are important in insect reproduction, but their anatomy and histology in R. neglectus are poorly studied. The aim of this work was to describe the histology and histochemistry of the accessory gland of the female reproductive tract of R. neglectus. The reproductive tract of five females of R. neglectus was dissected and the accessory glands transferred to Zamboni's fixative solution, dehydrated in a crescent series of ethanol, embedded in historesin, sectioned at 2 \u00b5m thick, stained with toluidine blue for histological analysis or mercury bromophenol blue for detection of total proteins. The accessory gland R. neglectus is tubular, without branches, opening in the dorsal region of the vagina and differing along its length in proximal and distal regions. In the proximal region, the gland is lined by the cuticle with a layer of columnar cells associated with muscle fibers. In the distal region of the gland, the epithelium has spherical secretory cells with terminal apparatus and conducting canaliculi opening in the lumen through pores in the cuticle. Proteins were identified in the gland lumen, terminal apparatus, nucleus and cytoplasm of secretory cells. The histology of the R. neglectus gland is similar to that found in other species of this genus, but with variations in the shape and size of its distal region.<\/jats:p>","DOI":"10.1590\/1519-6984.271913","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T14:33:46Z","timestamp":1684161226000},"source":"Crossref","is-referenced-by-count":3,"title":["Histology and histochemistry of the accessory gland of the female reproductive tract of Rhodnius neglectus Lent, 1954 (Hemiptera: Reduviidae)"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1003-9390","authenticated-orcid":false,"given":"D. S.","family":"Gomes","sequence":"first","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2211-7333","authenticated-orcid":false,"given":"C. J. F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Triangulo Mineiro, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2297-1919","authenticated-orcid":false,"given":"T. M. C.","family":"Costa","sequence":"additional","affiliation":[{"name":"Universidade Federal do Triangulo Mineiro, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2114-8237","authenticated-orcid":false,"given":"A. Plata","family":"Rueda","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Colombia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2026-281X","authenticated-orcid":false,"given":"J. C.","family":"Zanuncio","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0477-4252","authenticated-orcid":false,"given":"J. E.","family":"Serr\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1164-0591","authenticated-orcid":false,"given":"E. A.","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"7161","DOI":"10.1038\/s41598-020-64132-y","article-title":"Anatomical study of the female reproductive system and bacteriome of Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) using micro-computed tomography","volume":"10","author":"ALBA-ALEJANDRE I.","year":"2020","journal-title":"Scientific Reports"},{"key":"ref2","series-title":"Theory and practice of histological techniques.","author":"BANCROFT J.D.","year":"2008","edition":"6th"},{"issue":"7","key":"ref3","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1179\/136485905X51472","article-title":"Lipase and antibacterial activities of a recombinant protein from the accessory glands of female Phlebotomus papatasi (Diptera: psychodidae)","volume":"99","author":"BELARDINELLI M.","year":"2005","journal-title":"Annals of Tropical Medicine and Parasitology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.actatropica.2013.10.009","article-title":"A novel association between Rhodnius neglectus and the Livistona australis palm tree in na urban center foreshadowing the risk of Chagas disease transmission by vectorial invasions in Monte Alto City, S\u00e3o Paulo, Brazil","volume":"130","author":"CARVALHO D.B.","year":"2014","journal-title":"Acta Tropica"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13071-015-0634-7","article-title":"Immunosuppressive effects of Amblyomma cajennense tick saliva on murine bon\u00e9 marrow-derived dendritic cells","volume":"8","author":"CARVALHO-COSTA T.M.","year":"2015","journal-title":"Parasites & Vectors"},{"key":"ref6","series-title":"The Insects: structure and function.","volume":"5","author":"CHAPMAN R.F.","year":"2013"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jinsphys.2016.06.001","article-title":"Reproductive physiology in the blood feeding insect, Rhodnius prolixus, from copulation to the control of egg production","volume":"97","author":"CHIANG R.G.","year":"2017","journal-title":"Journal of Insect Physiology"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.asd.2009.06.002","article-title":"Functional anatomy of vagina muscles in the blood-feeding insect, Rhodnius prolixus.","volume":"38","author":"CHIANG R.G.","year":"2009","journal-title":"Arthropod Structure & Development"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.actatropica.2012.01.011","article-title":"Morphology of reproductive accessory glands in eight species of blood-feeding Hemiptera (Hemiptera, Reduviidae) insect vectors of Chagas disease","volume":"122","author":"CHIANG R.G.","year":"2012","journal-title":"Acta Tropica"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.jinsphys.2006.10.002","article-title":"The interaction of feeding and mating in the hormonal control of egg production in Rhodnius prolixus.","volume":"53","author":"DAVEY K.","year":"2007","journal-title":"Journal of Insect Physiology"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1590\/S1984-46702014000200007","article-title":"Gross morphology and ultrastructure of the female reproductive sistem of Diaphorina citri (Hemiptera: liviidae)","volume":"31","author":"DOSSI F.C.A.","year":"2014","journal-title":"Zoologia"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1603\/033.046.0430","article-title":"Predicting the Potential geographical distribution of Rhodnius neglectus (Hemiptera, Reduviidae) based on ecological niche modeling","volume":"46","author":"GURGEL-GON\u00c7ALVES R.","year":"2009","journal-title":"Journal of Medical Entomology"},{"key":"ref13","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/705326","article-title":"Geographic distribution of Chagas disease vectors in Brazil based on ecological niche modeling","volume":"2012","author":"GURGEL-GON\u00c7ALVES R.","year":"2012","journal-title":"Journal of Tropical Medicine"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1046\/j.1365-2915.2003.00448.x","article-title":"Sampling Rhodnius neglectus in Mauritia flexuosa palm trees: a field study in the Brazilian savanna","volume":"17","author":"GURGEL-GON\u00c7ALVES R.","year":"2003","journal-title":"Medical and Veterinary Entomology"},{"issue":"2A","key":"ref15","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1590\/S1519-69842006000300017","article-title":"Internal reproductive organs of Cosmoclopius nigroannulatus (Hemiptera: reduviidae)","volume":"66","author":"JAHNKE S.M.","year":"2006","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.asd.2015.04.003","article-title":"Reproductive biology in Anophelinae mosquitoes (Diptera, Culicidae): fine structure of the female accessory gland","volume":"44","author":"LAGHEZZA MASCI V.","year":"2015","journal-title":"Arthropod Structure & Development"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1590\/S1516-89132005000100017","article-title":"Morphology of female reproductive tract of the predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) fed on different diets","volume":"48","author":"LEMOS W.P.","year":"2005","journal-title":"Brazilian Archives of Biology and Technology"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/0040-8166(80)90030-0","article-title":"The development of the female accessory gland in the insect Rhodnius prolixus.","volume":"12","author":"LOCOCO D.","year":"1980","journal-title":"Tissue & Cell"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/0040-8166(80)90045-2","article-title":"The ultrastructure of the female accessory gland, the cement gland, in the insect Rhodnius prolixus.","volume":"12","author":"LOCOCO D.","year":"1980","journal-title":"Tissue & Cell"},{"key":"ref20","series-title":"Avalia\u00e7\u00e3o do efeito da saliva de triatom\u00edneos (Heteroptera: Reduviidae) sobre a biologia de c\u00e9lulas dendr\u00edticas murinas","author":"MENDES M.T.","year":"2014"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1590\/1519-6984.180932","article-title":"Morphology of the spermatheca of Triatoma lecticularia (Hem\u00edptera, Reduviidae) (Stal, 1859)","volume":"79","author":"MONTEIRO M.F.","year":"2019","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"key":"ref22","article-title":"Spermathecae: morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease","volume":"197","author":"NASCIMENTO J.D.","year":"2019","journal-title":"Acta Tropica"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.actatropica.2017.08.024","article-title":"Morphology of the spermathecae of twelve species of Triatominae (Hemiptera, Reduviidae) vectors of Chagas disease","volume":"176","author":"NASCIMENTO J.D.","year":"2017","journal-title":"Acta Tropica"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1080\/21686351.1991.12277630","article-title":"Glands, gland cells, glandular units: some comments on terminology and classification","volume":"27","author":"NOIROT C.","year":"1991","journal-title":"Annales de la Soci\u00e9t\u00e9 Entomologique de France"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.zool.2016.12.001","article-title":"The insect spermatheca: an overview","volume":"121","author":"PASCINI T.V.","year":"2017","journal-title":"Zoology"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1111\/j.1570-7458.2010.00986.x","article-title":"Age\u2010specific mortality analysis of the dry forest kissing bug, Rhodnius neglectus.","volume":"135","author":"RABINOVICH J.E.","year":"2010","journal-title":"Entomologia Experimentalis et Applicata"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1590\/0037-8682-0320-2017","article-title":"First report of Rhodnius neglectus (Hemiptera, Reduviidae, Triatominae) from the state of Acre, Brazil, and the Brazilian Western Amazon Region","volume":"51","author":"RAMOS L.J.","year":"2018","journal-title":"Revista da Sociedade Brasileira de Medicina Tropical"},{"issue":"63","key":"ref28","first-page":"20","article-title":"Detec\u00e7\u00e3o de triatom\u00edneos da esp\u00e9cie Rhodnius neglectus em \u00e1rea urbana de munic\u00edpios da regi\u00e3o de Ara\u00e7atuba","volume":"6","author":"RODRIGUES V.L.C.C.","year":"2009","journal-title":"Boletim Epidemiol\u00f3gico Paulista"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"62","DOI":"10.11646\/zootaxa.3478.1.8","article-title":"Description of Rhodnius montenegrensis n. sp. (Hemiptera: Reduviidae: Triatominae) from the state of Rond\u00f4nia, Brazil","volume":"3478","author":"ROSA J.A.","year":"2012","journal-title":"Zootaxa"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1365-2583.2003.00436.x","article-title":"A mammalian\u2010like lipase gene is expressed in the female reproductive accessory glands of the sand fly Phlebotomus papatasi (Diptera, Psychodidae)","volume":"12","author":"ROSETTO M.","year":"2003","journal-title":"Insect Molecular Biology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.peptides.2013.04.003","article-title":"The female reproductive system of the kissing bug, Rhodnius prolixus: arrangements of muscles, distribution and myoactivity of two endogenous FMRFamide-like peptides","volume":"53","author":"SEDRA L.","year":"2014","journal-title":"Peptides"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1093\/aesa\/sav097","article-title":"Morphology of the spermathecae of Leptoglossus zonatus (Heteroptera: coreidae)","volume":"109","author":"SOUZA E.A.","year":"2016","journal-title":"Annals of the Entomological Society of America"},{"issue":"2","key":"ref33","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1590\/S1519-566X2011000200011","article-title":"Ultrastructural and functional aspects of the spermatheca in the American Harlequin Bug, Murgantia histrionica (Hemiptera: pentatomidae)","volume":"40","author":"STACCONI M.V.R.","year":"2011","journal-title":"Neotropical Entomology"},{"issue":"5111","key":"ref34","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/216173a0","article-title":"Fixation of ejaculated spermatozoa for el\u00e9ctron miscroscopy","volume":"216","author":"STEFANINI M.","year":"1967","journal-title":"Nature"},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/mmnd.20020490203","article-title":"Morphology and ultrastructure of the female accessory sex glands in various crickets (Orthoptera, Saltatoria, Gryllidae)","volume":"49","author":"STURM R.","year":"2002","journal-title":"Deutsche Entomologische Zeitschrift"},{"issue":"1","key":"ref36","first-page":"35","article-title":"Morphology and histology of the ductus receptaculi and accessory glands in the reproductive tract of the female cricket, Teleogryllus commodus","volume":"8","author":"STURM R.","year":"2008","journal-title":"Journal of Insect Science"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1673\/031.012.9901","article-title":"Morphology and ultrastructure of the accessory glands in the female genital tract of the house cricket, Acheta domesticus.","volume":"12","author":"STURM R.","year":"2012","journal-title":"Journal of Insect Science"},{"issue":"2B","key":"ref38","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1590\/S1519-69842006000400014","article-title":"Phylogenetic approach to the study of triatomines (Triatominae, Heteroptera)","volume":"66","author":"TARTAROTTI E.","year":"2006","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.asd.2018.11.012","article-title":"Genital morphology and copulatory behavior in triatomine bugs (Reduviidae: triatominae)","volume":"49","author":"TELLEZ-GARCIA A.A.","year":"2019","journal-title":"Arthropod Structure & Development"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1093\/biolre\/ioaa147","article-title":"Structure of the reproductive system of the sexual generation of the endemic Arctic species Acyrthosiphon svalbardicum and its temperate counterpart Acyrthosiphon pisum (Hemiptera, Aphididae)","volume":"103","author":"WIECZOREK K.","year":"2020","journal-title":"Biology of Reproduction"},{"key":"ref41","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.7573","article-title":"The reproductive system of the male and oviparous female of a model organism - the pea aphid, Acyrthosiphon pisum (Hemiptera, Aphididae)","volume":"7","author":"WIECZOREK K.","year":"2019","journal-title":"PeerJ"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Histologia e histoqu\u00edmica das gl\u00e2ndulas acess\u00f3rias do sistema reprodutor feminino de Rhodnius neglectus Lent, 1954 (Hemiptera: Reduviidae)"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842023000100472&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T23:26:42Z","timestamp":1702423602000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842023000100472&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":41,"alternative-id":["S1519-69842023000100472"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.271913","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e271913"}}